AB151959

Anti-ACSL3 antibody

Name: Anti-ACSL3 antibody (ab151959) | Abcam
Brand: Abcam Limited
SKU: AB151959
Rating: 5 (1 reviews)

(1 Review)

(11 Publications)

Rabbit Polyclonal ACSL3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ACSL3 aa 400-650.

View Alternative Names

ACS3, FACL3, LACS3, ACSL3, Fatty acid CoA ligase Acsl3, Arachidonate--CoA ligase, Long-chain acyl-CoA synthetase 3, Long-chain-fatty-acid--CoA ligase 3, Medium-chain acyl-CoA ligase Acsl3, LACS 3

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL3 antibody (AB151959)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL3 antibody (AB151959)

Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labeling ACSL3 with ab151959 antibody at 1/500.

Immunocytochemistry/ Immunofluorescence - Anti-ACSL3 antibody (AB151959)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ACSL3 antibody (AB151959)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling ACSL3 protein at endoplasmic reticulum using ab151959 at a 1/500 dilution (Green).

Unknown

Western blot - Anti-ACSL3 antibody (AB151959)

7.5% SDS PAGE

All lanes:

Western blot - Anti-ACSL3 antibody (ab151959) at 1/1000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate at 30 µg

Predicted band size: 80 kDa

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Supplier Data

Western blot - Anti-ACSL3 antibody (AB151959)

Samples were separated by 7.5% SDS PAGE.

All lanes:

Western blot - Anti-ACSL3 antibody (ab151959) at 1/1000 dilution

Lane 1:

K562 whole cell extracts at 30 µg

Lane 2:

THP-1 whole cell extracts at 30 µg

Lane 3:

HL-60 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 80 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ACSL3 aa 400-650. The exact immunogen used to generate this antibody is proprietary information.

O95573

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ACSL3 protein also known as Acyl-CoA synthetase long-chain family member 3 plays a central role in lipid metabolism. It is an enzyme with a mass of approximately 79 kDa that activates long-chain fatty acids by converting them into acyl-CoA thioesters. This process is critical for their subsequent use in metabolic pathways. ACSL3 expression occurs mainly in the liver adipose tissue and brain tissues involved in energy balance and storage. By catalyzing the initial step in the fatty acid metabolic pathway ACSL3 influences lipid biosynthesis and degradation.

Biological function summary

ACSL3 contributes to cellular processes involving lipid synthesis and energy production. It functions as part of a larger lipid metabolic framework where it facilitates the incorporation of fatty acids into complex lipids like phospholipids and triglycerides. Though not a member of a molecular complex in terms of protein structure its activity complements other enzymes involved in lipid metabolism indicating an indirect association with lipid-binding proteins and transport mechanisms. The metabolic activity of ACSL3 therefore plays a significant role in maintaining cell membrane integrity and energy balance.

Pathways

ACSL3 integrates into the peroxisome proliferator-activated receptor (PPAR) signaling and fatty acid metabolism pathways. These pathways coordinate the regulation and utilization of lipids for energy storage and consumption. The ACSL3 protein interacts with proteins such as PPARα and PPARγ which are transcription factors that regulate gene expression associated with lipid metabolism. Through these interactions ACSL3 affects lipid metabolism at a genomic level promoting the adaptive responses necessary for cellular energy requirements.

ACSL3 association with metabolic conditions like obesity and non-alcoholic fatty liver disease (NAFLD) is evident. In obesity ACSL3 expression may alter lipid metabolism contributing to excess fat accumulation and energy imbalance. Additionally elevated ACSL3 levels in the liver could be linked to NAFLD enhancing lipid storage and steatosis. The protein interacts indirectly with other players in metabolic diseases such as SREBP-1c and AMPK which are critical regulators of lipid homeostasis and energy balance in cells. These connections suggest that ACSL3 is a potential target for therapeutic interventions in metabolic disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acyl-CoA synthetases (ACSL) activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation (PubMed : 22633490). Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins) (PubMed : 18003621). Has mainly an anabolic role in energy metabolism. Mediates hepatic lipogenesis. Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates. Both isoforms exhibit the same level of activity (By similarity).

See full target information ACSL3

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Discover oncology 15:670 PubMed39556281

2024

Circ_0124346 facilitates cell proliferation of pancreatic adenocarcinoma cells by regulating lipid metabolism via miR-223-3p/ACSL3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Lu Shu,Wan-Ting Yang,Hui-Min Li,Cui-Juan Qian,Xiao-Sheng Teng,Jun Yao

EMBO molecular medicine 16:2749-2774 PubMed39433871

2024

Breast cancer secretes anti-ferroptotic MUFAs and depends on selenoprotein synthesis for metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Tobias Ackermann,Engy Shokry,Ruhi Deshmukh,Jayanthi Anand,Laura C A Galbraith,Louise Mitchell,Giovanny Rodriguez-Blanco,Victor H Villar,Britt Amber Sterken,Colin Nixon,Sara Zanivan,Karen Blyth,David Sumpton,Saverio Tardito

Nature communications 15:7611 PubMed39218970

2024

Suppression of ferroptosis by vitamin A or radical-trapping antioxidants is essential for neuronal development.

Applications

Unspecified application

Species

Unspecified reactive species

Juliane Tschuck,Vidya Padmanabhan Nair,Ana Galhoz,Carole Zaratiegui,Hin-Man Tai,Gabriele Ciceri,Ina Rothenaigner,Jason Tchieu,Brent R Stockwell,Lorenz Studer,Daphne S Cabianca,Michael P Menden,Michelle Vincendeau,Kamyar Hadian

Cell chemical biology 31:249-264.e7 PubMed37944523

2023

Ferroptosis inhibition by oleic acid mitigates iron-overload-induced injury.

Applications

Unspecified application

Species

Unspecified reactive species

Josiane Mann,Eduard Reznik,Melania Santer,Mark A Fongheiser,Nailah Smith,Tal Hirschhorn,Fereshteh Zandkarimi,Rajesh Kumar Soni,Alcir Luiz Dafré,Antonio Miranda-Vizuete,Marcelo Farina,Brent R Stockwell

Aging 15:4699-4713 PubMed37294538

2023

Pan-cancer analysis identifies LPCATs family as a prognostic biomarker and validation of LPCAT4/WNT/β-catenin/c-JUN/ACSL3 in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yaoyong Lu,Hongfeng Liang,Xiaoyin Li,Haiwen Chen,Changfu Yang

Cell death & disease 14:182 PubMed36878903

2023

USP7- and PRMT5-dependent G3BP2 stabilization drives de novo lipogenesis and tumorigenesis of HNSC.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Wang,Tianzi Li,Wanyu Liu,Jinhua Lin,Ke Zhang,Zhenhao Li,Yanfei Huang,Yufei Shi,Meilan Xu,Xuekui Liu

Nature communications 13:1991 PubMed35418170

2022

The nuclear receptor ERR cooperates with the cardiogenic factor GATA4 to orchestrate cardiomyocyte maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Tomoya Sakamoto,Kirill Batmanov,Shibiao Wan,Yuanjun Guo,Ling Lai,Rick B Vega,Daniel P Kelly

Molecular cancer research : MCR 19:124-135 PubMed33077484

2020

Long-Chain Acyl-CoA Synthetase 4-Mediated Fatty Acid Metabolism Sustains Androgen Receptor Pathway-Independent Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yongjie Ma,Xiaohan Zhang,Omar Awad Alsaidan,Xiangkun Yang,Essilvo Sulejmani,Junyi Zha,Zanna Beharry,Hanwen Huang,Michael Bartlett,Zachary Lewis,Houjian Cai

Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association 23:241-259 PubMed31520166

2019

The modification of ferroptosis and abnormal lipometabolism through overexpression and knockdown of potential prognostic biomarker perilipin2 in gastric carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoying Sun,Shaojuan Yang,Xuechao Feng,Yaowu Zheng,Jinsong Zhou,Hai Wang,Yucheng Zhang,Hongyan Sun,Chengyan He

Molecular omics 14:181-196 PubMed29770421

2018

Quantitative proteomics and systems analysis of cultured H9C2 cardiomyoblasts during differentiation over time supports a 'function follows form' model of differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Cynthia Kankeu,Kylie Clarke,Delphi Van Haver,Kris Gevaert,Francis Impens,Anna Dittrich,H Llewelyn Roderick,Egle Passante,Heinrich J Huber

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ACSL3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL3 antibody (AB151959)

Immunocytochemistry/ Immunofluorescence - Anti-ACSL3 antibody (AB151959)

Western blot - Anti-ACSL3 antibody (AB151959)

Western blot - Anti-ACSL3 antibody (AB151959)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Product promise