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AB321811

Anti-ACSL3 antibody [EPR29199-39]

  • 20ul selling size
  • Recombinant
  • RabMAb
  • KO Validated
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Rabbit Recombinant Monoclonal ACSL3 antibody. Suitable for WB and reacts with Human, Mouse samples.
3 Images
Western blot - Anti-ACSL3 antibody [EPR29199-39] (AB321811)
  • WB

Supplier Data

Western blot - Anti-ACSL3 antibody [EPR29199-39] (AB321811)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the bands at around 30 kDa & 25 kDa (Lane 1&3, respectively) are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ACSL3 antibody [EPR29199-39] (ab321811) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 20 µg

Lane 2:

Human testis tissue lysate at 20 µg

Lane 3:

Mouse brain tissue lysate at 20 µg

Lane 4:

Mouse testis tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 80 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-ACSL3 antibody [EPR29199-39] (AB321811)
  • WB

Supplier Data

Western blot - Anti-ACSL3 antibody [EPR29199-39] (AB321811)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the band at around 20 kDa (Lane 2) is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ACSL3 antibody [EPR29199-39] (ab321811) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) whole lysate at 20 µg

Lane 2:

LNCaP (human prostate carcinoma epithelial cell) whole lysate at 20 µg

Lane 3:

MCF7 (human breast adenocarcinoma epithelial cell) whole lysate at 20 µg

Lane 4:

MEF (mouse embryo fibroblast) whole lysate at 20 µg

Lane 5:

NIH/3T3 (mouse embryonic fibroblast) whole lysate at 20 µg

Lane 6:

Neuro-2a (mouse neuroblastoma neuroblast) whole lysate at 20 µg

Lane 7:

4T1 (mouse mammary gland carcinoma epithelial cell) whole lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

Western blot - Anti-ACSL3 antibody [EPR29199-39] (AB321811)
  • WB

Supplier Data

Western blot - Anti-ACSL3 antibody [EPR29199-39] (AB321811)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, ab321811 was shown to bind specifically to ACSL3. Target of interest was observed at 80 kDa in wild-type HeLa cell lysates (Lane 1) with no signal observed at this size in ACSL3 knockout cell line (ab265102) (Lane 2).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ACSL3 antibody [EPR29199-39] (ab321811) at 1/1000 dilution

Lane 1:

Parental HeLa(EDWT03) whole lysate at 20 µg

Lane 2:

ACSL3 KO HeLa (ED030312) whole lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 80 kDa,36 kDa

false

Exposure time: 180s

  • Carrier free

    Anti-ACSL3 antibody [EPR29199-39] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29199-39

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ACSL3 protein also known as Acyl-CoA synthetase long-chain family member 3 plays a central role in lipid metabolism. It is an enzyme with a mass of approximately 79 kDa that activates long-chain fatty acids by converting them into acyl-CoA thioesters. This process is critical for their subsequent use in metabolic pathways. ACSL3 expression occurs mainly in the liver adipose tissue and brain tissues involved in energy balance and storage. By catalyzing the initial step in the fatty acid metabolic pathway ACSL3 influences lipid biosynthesis and degradation.
Biological function summary

ACSL3 contributes to cellular processes involving lipid synthesis and energy production. It functions as part of a larger lipid metabolic framework where it facilitates the incorporation of fatty acids into complex lipids like phospholipids and triglycerides. Though not a member of a molecular complex in terms of protein structure its activity complements other enzymes involved in lipid metabolism indicating an indirect association with lipid-binding proteins and transport mechanisms. The metabolic activity of ACSL3 therefore plays a significant role in maintaining cell membrane integrity and energy balance.

Pathways

ACSL3 integrates into the peroxisome proliferator-activated receptor (PPAR) signaling and fatty acid metabolism pathways. These pathways coordinate the regulation and utilization of lipids for energy storage and consumption. The ACSL3 protein interacts with proteins such as PPARα and PPARγ which are transcription factors that regulate gene expression associated with lipid metabolism. Through these interactions ACSL3 affects lipid metabolism at a genomic level promoting the adaptive responses necessary for cellular energy requirements.

ACSL3 association with metabolic conditions like obesity and non-alcoholic fatty liver disease (NAFLD) is evident. In obesity ACSL3 expression may alter lipid metabolism contributing to excess fat accumulation and energy imbalance. Additionally elevated ACSL3 levels in the liver could be linked to NAFLD enhancing lipid storage and steatosis. The protein interacts indirectly with other players in metabolic diseases such as SREBP-1c and AMPK which are critical regulators of lipid homeostasis and energy balance in cells. These connections suggest that ACSL3 is a potential target for therapeutic interventions in metabolic disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acyl-CoA synthetases (ACSL) activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation (PubMed : 22633490). Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins) (PubMed : 18003621). Has mainly an anabolic role in energy metabolism. Mediates hepatic lipogenesis. Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates. Both isoforms exhibit the same level of activity (By similarity).
See full target information ACSL3

Alternative Names

ACS3, FACL3, LACS3, ACSL3, Fatty acid CoA ligase Acsl3, Arachidonate--CoA ligase, Long-chain acyl-CoA synthetase 3, Long-chain-fatty-acid--CoA ligase 3, Medium-chain acyl-CoA ligase Acsl3, LACS 3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com