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AB321812

Anti-ACSL3 antibody [EPR29199-39] - BSA and Azide free

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Rabbit Recombinant Monoclonal ACSL3 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse samples.

View Alternative Names

ACS3, FACL3, LACS3, ACSL3, Fatty acid CoA ligase Acsl3, Arachidonate--CoA ligase, Long-chain acyl-CoA synthetase 3, Long-chain-fatty-acid--CoA ligase 3, Medium-chain acyl-CoA ligase Acsl3, LACS 3

1 Images
Western blot - Anti-ACSL3 antibody [EPR29199-39] - BSA and Azide free (AB321812)
  • WB

Supplier Data

Western blot - Anti-ACSL3 antibody [EPR29199-39] - BSA and Azide free (AB321812)

This data was developed using ab321811, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Performed under reducing conditions.

In Western blot, ab321811 was shown to bind specifically to ACSL3. Target of interest was observed at 80 kDa in wild-type HeLa cell lysates (Lane 1) with no signal observed at this size in ACSL3 knockout cell line (ab265102) (Lane 2).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ACSL3 antibody [EPR29199-39] (<a href='/en-us/products/primary-antibodies/acsl3-antibody-epr29199-39-ab321811'>ab321811</a>) at 1/1000 dilution

Lane 1:

Parental HeLa(EDWT03) whole lysate at 20 µg

Lane 2:

ACSL3 KO HeLa (ED030312) whole lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 80 kDa,36 kDa

false

Exposure time: 180s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29199-39

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab321812 is the carrier-free version of ab321811.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ACSL3 protein also known as Acyl-CoA synthetase long-chain family member 3 plays a central role in lipid metabolism. It is an enzyme with a mass of approximately 79 kDa that activates long-chain fatty acids by converting them into acyl-CoA thioesters. This process is critical for their subsequent use in metabolic pathways. ACSL3 expression occurs mainly in the liver adipose tissue and brain tissues involved in energy balance and storage. By catalyzing the initial step in the fatty acid metabolic pathway ACSL3 influences lipid biosynthesis and degradation.
Biological function summary

ACSL3 contributes to cellular processes involving lipid synthesis and energy production. It functions as part of a larger lipid metabolic framework where it facilitates the incorporation of fatty acids into complex lipids like phospholipids and triglycerides. Though not a member of a molecular complex in terms of protein structure its activity complements other enzymes involved in lipid metabolism indicating an indirect association with lipid-binding proteins and transport mechanisms. The metabolic activity of ACSL3 therefore plays a significant role in maintaining cell membrane integrity and energy balance.

Pathways

ACSL3 integrates into the peroxisome proliferator-activated receptor (PPAR) signaling and fatty acid metabolism pathways. These pathways coordinate the regulation and utilization of lipids for energy storage and consumption. The ACSL3 protein interacts with proteins such as PPARα and PPARγ which are transcription factors that regulate gene expression associated with lipid metabolism. Through these interactions ACSL3 affects lipid metabolism at a genomic level promoting the adaptive responses necessary for cellular energy requirements.

ACSL3 association with metabolic conditions like obesity and non-alcoholic fatty liver disease (NAFLD) is evident. In obesity ACSL3 expression may alter lipid metabolism contributing to excess fat accumulation and energy imbalance. Additionally elevated ACSL3 levels in the liver could be linked to NAFLD enhancing lipid storage and steatosis. The protein interacts indirectly with other players in metabolic diseases such as SREBP-1c and AMPK which are critical regulators of lipid homeostasis and energy balance in cells. These connections suggest that ACSL3 is a potential target for therapeutic interventions in metabolic disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acyl-CoA synthetases (ACSL) activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation (PubMed : 22633490). Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins) (PubMed : 18003621). Has mainly an anabolic role in energy metabolism. Mediates hepatic lipogenesis. Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates. Both isoforms exhibit the same level of activity (By similarity).
See full target information ACSL3
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Product promise

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