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AB272556

Anti-ACSL5 antibody

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(2 Publications)

Rabbit Polyclonal ACSL5 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ACSL5 aa 150-300.

View Alternative Names

ACS5, FACL5, UNQ633/PRO1250, ACSL5, Long-chain-fatty-acid--CoA ligase 5, Arachidonate--CoA ligase, Long-chain acyl-CoA synthetase 5, LACS 5

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL5 antibody (AB272556)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL5 antibody (AB272556)

Paraffin-embedded formalin-fixed human pancreas tissue stained for ACSL5 using ab272556 at 1/20 dilution in immunohistochemical analysis. Shows low expression as expected.

Immunocytochemistry/ Immunofluorescence - Anti-ACSL5 antibody (AB272556)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ACSL5 antibody (AB272556)

PFA fixed, Triton X-100 permeablized U-251 MG (human brain glioma cell line) whole cell lysate labeling ACSL5 (green) using ab272556 at 2 ug/ml in ICC/IF analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL5 antibody (AB272556)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL5 antibody (AB272556)

Paraffin-embedded formalin-fixed human small intestine tissue stained for ACSL5 using ab272556 at 1/20 dilution in immunohistochemical analysis.

Western blot - Anti-ACSL5 antibody (AB272556)
  • WB

Unknown

Western blot - Anti-ACSL5 antibody (AB272556)

All lanes:

Western blot - Anti-ACSL5 antibody (ab272556) at 0.4 µg/mL

All lanes:

CAPAN-2 (human pancreatic ductal adenocarcinoma cell line) whole cell lysate

Predicted band size: 76 kDa

false

Western blot - Anti-ACSL5 antibody (AB272556)
  • WB

Unknown

Western blot - Anti-ACSL5 antibody (AB272556)

All lanes:

Western blot - Anti-ACSL5 antibody (ab272556) at 0.4 µg/mL

Lane 1:

Vector only transfected HEK293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate

Lane 2:

ACSL5 over-expression HEK293T lysate

Predicted band size: 76 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ACSL5 aa 150-300. The exact immunogen used to generate this antibody is proprietary information.

Q9ULC5

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 + ACSL6 antibody [EPR13499] (AB177958)

  • 4
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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ACSL5 also known as acyl-CoA synthetase long-chain family member 5 is an enzyme with a mass of approximately 78 kDa. This enzyme plays a critical role in lipid metabolism converting free long-chain fatty acids into fatty acyl-CoA esters ready for incorporation into complex lipids or for energy production. ACSL5 is expressed in various tissues with significant levels detected in liver adipose tissue and skeletal muscle highlighting its importance in metabolic functions.
Biological function summary

This enzyme participates in the activation of fatty acids an important step in lipid biosynthesis and degradation. ACSL5 is part of the larger acyl-CoA synthetase family known for its involvement in lipid metabolism. By converting fatty acids into their acyl-CoA derivatives it provides substrates for lipid synthesis and β-oxidation enabling efficient energy utilization and storage in cells.

Pathways

ACSL5 integrates into both the lipogenesis and β-oxidation pathways. These pathways are essential for maintaining energy balance and lipid homeostasis in cells. In lipogenesis ACSL5 assists in the synthesis of complex lipids by providing activated fatty acids. During β-oxidation it collaborates with enzymes like CPT1 which transports fatty acids into mitochondria for degradation illustrating ACSL5’s integral role in these metabolic processes.

Disruptions in ACSL5 function have associations with metabolic disorders like obesity and non-alcoholic fatty liver disease. These conditions often involve imbalances in lipid processing and storage. ACSL5's interactions with proteins like PPARα a transcription factor involved in lipid metabolism regulation further highlight its relevance in these disorders as alterations in ACSL5 expression can influence PPARα-related lipid metabolic pathways potentially leading to or exacerbating these metabolic diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation (PubMed : 17681178, PubMed : 22633490, PubMed : 24269233, PubMed : 33191500). ACSL5 may activate fatty acids from exogenous sources for the synthesis of triacylglycerol destined for intracellular storage (By similarity). Utilizes a wide range of saturated fatty acids with a preference for C16-C18 unsaturated fatty acids (By similarity). It was suggested that it may also stimulate fatty acid oxidation (By similarity). At the villus tip of the crypt-villus axis of the small intestine may sensitize epithelial cells to apoptosis specifically triggered by the death ligand TRAIL. May have a role in the survival of glioma cells.
See full target information ACSL5
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112742 PubMed40546938

2025

Interferon gamma induced-ACSL5 shapes the lipidome of kidney tubular cells.

Applications

Unspecified application

Species

Unspecified reactive species

Virginie Poindessous,Julio L Sampaio,Lydia Bouidghaghen,Ivan Nemazanyy,Alexandre Pallet,Maarten Naesens,Thibaut Vaulet,Dany Anglicheau,Nicolas Pallet

European journal of histochemistry : EJH 67: PubMed37946526

2023

Regulatory roles of ACSL5 in the anti-tumor function of palmitic acid (C16:0) <em>via</em> the ERK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jiapei Lv,Wang Yanting,Shan Wei
View all publications
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Product promise

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