AB229958

Anti-ACSL6/ACS2 antibody

Name: Anti-ACSL6/ACS2 antibody (ab229958) | Abcam
Brand: Abcam Limited
SKU: AB229958

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(4 Publications)

Rabbit Polyclonal ACSL6/ACS2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ACSL6 aa 450 to C-terminus.

View Alternative Names

ACS2, FACL6, KIAA0837, LACS5, ACSL6, Long-chain-fatty-acid--CoA ligase 6, Arachidonate--CoA ligase, Long-chain acyl-CoA synthetase 6, LACS 6

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL6/ACS2 antibody (AB229958)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL6/ACS2 antibody (AB229958)

Paraffin-embedded human adrenal gland tissue stained for ACSL6/ACS2 using ab229958 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL6/ACS2 antibody (AB229958)

Paraffin-embedded human brain tissue stained for ACSL6/ACS2 using ab229958 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-ACSL6/ACS2 antibody (AB229958)

All lanes:

Western blot - Anti-ACSL6/ACS2 antibody (ab229958) at 1/1000 dilution

Lane 1:

Mouse liver lysate

Lane 2:

Mouse heart lysate

Lane 3:

Mouse brain lysate

Lane 4:

Mouse gonadal lysate

Lane 5:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

Lane 6:

Raji (human Burkitt's lymphoma cell line) whole cell lysate

Lane 7:

K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 78 kDa

Observed band size: 78 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ACSL6 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UKU0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ACSL6 also known as ACS2 is an enzyme involved in lipid metabolism. It is responsible for converting long-chain fatty acids into acyl-CoA an essential step in the metabolism of fatty acids. ACSL6 has a molecular mass that ranges around 78 kilodaltons. It is expressed in the brain particularly in neurons and can also be found in other tissues like the heart and skeletal muscles. This enzyme is important for maintaining energy balance and proper neural function due to its role in lipid metabolism.

Biological function summary

The activation of long-chain fatty acids by ACSL6 is an important step facilitating their incorporation into cellular lipids or their oxidation to generate energy. While ACSL6 does not form part of a multi-protein complex its activity aids in the regulation of lipidic composition within membranes and signaling molecules. ACSL6 plays a role in the maintenance of membrane fluidity which is important for neuronal health and function.

Pathways

The role of ACSL6 in the metabolism of fatty acids intersects with the beta-oxidation and lipid biosynthesis pathways. ACSL6 cooperates with other ACSL family members such as ACSL1 in these pathways though each family member may have different substrate specificities. Fatty acid oxidation represents an important energy source especially in tissues with high demand like the heart and brain where ACSL6 is notably expressed.

Disruptions in ACSL6 activity have been associated with neurological disorders and metabolic diseases. For example aberrations in ACSL6 expression or function have been linked to schizophrenia where altered lipid metabolism may affect neural cell membranes and signaling. ACSL6's connection to schizophrenia involves interactions with pathways also influenced by proteins like COMT which is implicated in cognitive functions and neurochemistry. Such links suggest that ACSL6's proper functioning is necessary for maintaining normal physiological conditions in the brain and perhaps in associated metabolic contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoA for both synthesis of cellular lipids, and degradation via beta-oxidation (PubMed : 22633490, PubMed : 24269233). Plays an important role in fatty acid metabolism in brain and the acyl-CoAs produced may be utilized exclusively for the synthesis of the brain lipid.

See full target information ACSL6

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113311 PubMed40917882

2025

An iPSC-derived neuronal model reveals manganese's role in neuronal endocytosis, calcium flux and mitochondrial bioenergetics.

Applications

Unspecified application

Species

Unspecified reactive species

Dimitri Budinger,Sharmin Alhaque,Ramón González-Méndez,Chris Dadswell,Katy Barwick,Arianna Ferrini,Charlotte Roth,Conor J McCann,Karin Tuschl,Fatma Al Jasmi,Maha S Zaki,Julien H Park,Russell C Dale,Shekeeb Mohammad,John Christodoulou,Dale Moulding,Michael R Duchen,Serena Barral,Manju A Kurian

Molecular cancer 24:29 PubMed39856701

2025

Circular RNA circBNC2 inhibits tumorigenesis by modulating ferroptosis and acts as a nanotherapeutic target in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Pan,Kailai Chen,Wei Gao,Meiqi Xu,Fanlong Meng,Mengyuan Wu,Zi Qi Wang,Yun Qi Li,Wanhai Xu,Manjie Zhang,Yakun Luo

Frontiers in genetics 13:971364 PubMed36160009

2022

A ferroptosis associated gene signature for predicting prognosis and immune responses in patients with colorectal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lijun Yan,Xi Chen,Zhaolian Bian,Chunyan Gu,Hanzhen Ji,Liyan Chen,Haifeng Xu,Qiyun Tang

Frontiers in pharmacology 12:594437 PubMed33912032

2021

5-Bis-(2,6-difluoro-benzylidene) Cyclopentanone Acts as a Selective 11β-Hydroxysteroid Dehydrogenase one Inhibitor to Treat Diet-Induced Nonalcoholic Fatty Liver Disease in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hongguo Guan,Yiyan Wang,Huitao Li,Qiqi Zhu,Xiaoheng Li,Guang Liang,Ren-Shan Ge

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-ACSL6/ACS2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL6/ACS2 antibody (AB229958)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL6/ACS2 antibody (AB229958)

Western blot - Anti-ACSL6/ACS2 antibody (AB229958)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise