AB317703

Anti-ACSL6/ACS2 antibody [EPR29174-76]

20ul selling size
Recombinant
RabMAb
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Rabbit Recombinant Monoclonal ACSL6/ACS2 antibody. Suitable for WB, IP and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.

View Alternative Names

ACS2, FACL6, KIAA0837, LACS5, Long-chain-fatty-acid--CoA ligase 6, Arachidonate--CoA ligase, Long-chain acyl-CoA synthetase 6, LACS 6

5 Images

Supplier Data

Immunoprecipitation - Anti-ACSL6/ACS2 antibody [EPR29174-76] (AB317703)

ACSL6/ACS2 was immunoprecipitated from 0.35 mg Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole lysate with ab317703 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317703 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole lysate

Lane 2 : ab317703 IP in Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab317703 in Jurkat whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Immunoprecipitation - Anti-ACSL6/ACS2 antibody [EPR29174-76] (ab317703) at 1/30 dilution

All lanes:

Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

true

Exposure time: 180s

Supplier Data

Western blot - Anti-ACSL6/ACS2 antibody [EPR29174-76] (AB317703)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ACSL6/ACS2 antibody [EPR29174-76] (ab317703) at 1/1000 dilution

All lanes:

Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 74 kDa,36 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-ACSL6/ACS2 antibody [EPR29174-76] (AB317703)

Low expression : liver, lung (PMID : 12767919, PMID : 10502316).

The identity of the band at approximately 37 kDa (in lane 4) is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ACSL6/ACS2 antibody [EPR29174-76] (ab317703) at 1/1000 dilution

Lane 1:

Human hypothalamus tissue lysate at 50 µg

Lane 2:

Human cerebellum tissue lysate at 50 µg

Lane 3:

Human lung tissue lysate at 50 µg

Lane 4:

Human liver tissue lysate at 50 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 74 kDa,36 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-ACSL6/ACS2 antibody [EPR29174-76] (AB317703)

Low expression : liver, kidney (PMID : 12767919, PMID : 10502316).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ACSL6/ACS2 antibody [EPR29174-76] (ab317703) at 1/1000 dilution

Lane 1:

Rat brain tissue lysate at 20 µg

Lane 2:

Rat liver tissue lysate at 20 µg

Lane 3:

Mouse brain tissue lysate at 20 µg

Lane 4:

Mouse hypothalamus tissue lysate at 20 µg

Lane 5:

Mouse kidney tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 74 kDa,36 kDa

true

Exposure time: 180s

Supplier Data

Western blot - Anti-ACSL6/ACS2 antibody [EPR29174-76] (AB317703)

This antibody does not cross-react with human ACSL1 and ACSL5.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

In Western blot, Anti-GST antibody [EPR4236] (ab111947) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-ACSL6/ACS2 antibody [EPR29174-76] (ab317703) at 1/1000 dilution

Lane 1:

His-tagged human ACSL6/ACS2 fragment at 10 ng

Lane 2:

GST-tagged human ACSL5 fragment at 10 ng

Lane 3:

His-tagged human ACSL1 fragment at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 15 kDa,37 kDa

false

Exposure time: 180s

Carrier free

Anti-ACSL6/ACS2 antibody [EPR29174-76] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29174-76

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ACSL6 also known as ACS2 is an enzyme involved in lipid metabolism. It is responsible for converting long-chain fatty acids into acyl-CoA an essential step in the metabolism of fatty acids. ACSL6 has a molecular mass that ranges around 78 kilodaltons. It is expressed in the brain particularly in neurons and can also be found in other tissues like the heart and skeletal muscles. This enzyme is important for maintaining energy balance and proper neural function due to its role in lipid metabolism.

Biological function summary

The activation of long-chain fatty acids by ACSL6 is an important step facilitating their incorporation into cellular lipids or their oxidation to generate energy. While ACSL6 does not form part of a multi-protein complex its activity aids in the regulation of lipidic composition within membranes and signaling molecules. ACSL6 plays a role in the maintenance of membrane fluidity which is important for neuronal health and function.

Pathways

The role of ACSL6 in the metabolism of fatty acids intersects with the beta-oxidation and lipid biosynthesis pathways. ACSL6 cooperates with other ACSL family members such as ACSL1 in these pathways though each family member may have different substrate specificities. Fatty acid oxidation represents an important energy source especially in tissues with high demand like the heart and brain where ACSL6 is notably expressed.

Disruptions in ACSL6 activity have been associated with neurological disorders and metabolic diseases. For example aberrations in ACSL6 expression or function have been linked to schizophrenia where altered lipid metabolism may affect neural cell membranes and signaling. ACSL6's connection to schizophrenia involves interactions with pathways also influenced by proteins like COMT which is implicated in cognitive functions and neurochemistry. Such links suggest that ACSL6's proper functioning is necessary for maintaining normal physiological conditions in the brain and perhaps in associated metabolic contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoA for both synthesis of cellular lipids, and degradation via beta-oxidation (PubMed : 22633490, PubMed : 24269233). Plays an important role in fatty acid metabolism in brain and the acyl-CoAs produced may be utilized exclusively for the synthesis of the brain lipid.

See full target information Long-chain-fatty-acid--CoA ligase 6

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ACSL6/ACS2 antibody [EPR29174-76]

Immunoprecipitation - Anti-ACSL6/ACS2 antibody [EPR29174-76] (AB317703)

Western blot - Anti-ACSL6/ACS2 antibody [EPR29174-76] (AB317703)

Western blot - Anti-ACSL6/ACS2 antibody [EPR29174-76] (AB317703)

Western blot - Anti-ACSL6/ACS2 antibody [EPR29174-76] (AB317703)

Western blot - Anti-ACSL6/ACS2 antibody [EPR29174-76] (AB317703)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise