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Rabbit Recombinant Monoclonal ACSL6/ACS2 antibody. Suitable for WB, IP and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.

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Images

Immunoprecipitation - Anti-ACSL6/ACS2 antibody [EPR29174-76] (AB317703), expandable thumbnail
  • Western blot - Anti-ACSL6/ACS2 antibody [EPR29174-76] (AB317703), expandable thumbnail
  • Western blot - Anti-ACSL6/ACS2 antibody [EPR29174-76] (AB317703), expandable thumbnail
  • Western blot - Anti-ACSL6/ACS2 antibody [EPR29174-76] (AB317703), expandable thumbnail
  • Western blot - Anti-ACSL6/ACS2 antibody [EPR29174-76] (AB317703), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPIHC-PICC/IF
Human
Tested
Tested
Not recommended
Not recommended
Mouse
Tested
Expected
Not recommended
Not recommended
Rat
Tested
Expected
Not recommended
Not recommended
Recombinant fragment - Human
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Recombinant fragment - Human
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Recombinant fragment - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Recombinant fragment - Human
Dilution info
-
Notes

-

Target data

Function

Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoA for both synthesis of cellular lipids, and degradation via beta-oxidation (PubMed:22633490, PubMed:24269233). Plays an important role in fatty acid metabolism in brain and the acyl-CoAs produced may be utilized exclusively for the synthesis of the brain lipid.

Alternative names

ACS2, FACL6, KIAA0837, LACS5, Long-chain-fatty-acid--CoA ligase 6, Arachidonate--CoA ligase, Long-chain acyl-CoA synthetase 6, LACS 6

Recommended products

Rabbit Recombinant Monoclonal ACSL6/ACS2 antibody. Suitable for WB, IP and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR29174-76
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The ACSL6 also known as ACS2 is an enzyme involved in lipid metabolism. It is responsible for converting long-chain fatty acids into acyl-CoA an essential step in the metabolism of fatty acids. ACSL6 has a molecular mass that ranges around 78 kilodaltons. It is expressed in the brain particularly in neurons and can also be found in other tissues like the heart and skeletal muscles. This enzyme is important for maintaining energy balance and proper neural function due to its role in lipid metabolism.

Biological function summary

The activation of long-chain fatty acids by ACSL6 is an important step facilitating their incorporation into cellular lipids or their oxidation to generate energy. While ACSL6 does not form part of a multi-protein complex its activity aids in the regulation of lipidic composition within membranes and signaling molecules. ACSL6 plays a role in the maintenance of membrane fluidity which is important for neuronal health and function.

Pathways

The role of ACSL6 in the metabolism of fatty acids intersects with the beta-oxidation and lipid biosynthesis pathways. ACSL6 cooperates with other ACSL family members such as ACSL1 in these pathways though each family member may have different substrate specificities. Fatty acid oxidation represents an important energy source especially in tissues with high demand like the heart and brain where ACSL6 is notably expressed.

Associated diseases and disorders

Disruptions in ACSL6 activity have been associated with neurological disorders and metabolic diseases. For example aberrations in ACSL6 expression or function have been linked to schizophrenia where altered lipid metabolism may affect neural cell membranes and signaling. ACSL6's connection to schizophrenia involves interactions with pathways also influenced by proteins like COMT which is implicated in cognitive functions and neurochemistry. Such links suggest that ACSL6's proper functioning is necessary for maintaining normal physiological conditions in the brain and perhaps in associated metabolic contexts.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunoprecipitation - Anti-ACSL6/ACS2 antibody [EPR29174-76] (ab317703), expandable thumbnail

    Immunoprecipitation - Anti-ACSL6/ACS2 antibody [EPR29174-76] (ab317703)

    ACSL6/ACS2 was immunoprecipitated from 0.35 mg Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole lysate with ab317703 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317703 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole lysate

    Lane 2: ab317703 IP in Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab317703 in Jurkat whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    All lanes: Immunoprecipitation - Anti-ACSL6/ACS2 antibody [EPR29174-76] (ab317703) at 1/30 dilution

    All lanes: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Developed using the ECL technique.

    Exposure time: 180s

  • Western blot - Anti-ACSL6/ACS2 antibody [EPR29174-76] (ab317703), expandable thumbnail

    Western blot - Anti-ACSL6/ACS2 antibody [EPR29174-76] (ab317703)

    Low expression: liver, lung (PMID: 12767919, PMID: 10502316).

    The identity of the band at approximately 37 kDa (in lane 4) is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-ACSL6/ACS2 antibody [EPR29174-76] (ab317703) at 1/1000 dilution

    Lane 1: Human hypothalamus tissue lysate at 50 µg with NFDM/TBST

    Lane 2: Human cerebellum tissue lysate at 50 µg with NFDM/TBST

    Lane 3: Human lung tissue lysate at 50 µg with NFDM/TBST

    Lane 4: Human liver tissue lysate at 50 µg with NFDM/TBST

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 74 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-ACSL6/ACS2 antibody [EPR29174-76] (ab317703), expandable thumbnail

    Western blot - Anti-ACSL6/ACS2 antibody [EPR29174-76] (ab317703)

    Low expression: liver, kidney (PMID: 12767919, PMID: 10502316).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-ACSL6/ACS2 antibody [EPR29174-76] (ab317703) at 1/1000 dilution

    Lane 1: Rat brain tissue lysate at 20 µg with NFDM/TBST

    Lane 2: Rat liver tissue lysate at 20 µg with NFDM/TBST

    Lane 3: Mouse brain tissue lysate at 20 µg with NFDM/TBST

    Lane 4: Mouse hypothalamus tissue lysate at 20 µg with NFDM/TBST

    Lane 5: Mouse kidney tissue lysate at 20 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Observed band size: 74 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-ACSL6/ACS2 antibody [EPR29174-76] (ab317703), expandable thumbnail

    Western blot - Anti-ACSL6/ACS2 antibody [EPR29174-76] (ab317703)

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-ACSL6/ACS2 antibody [EPR29174-76] (ab317703) at 1/1000 dilution

    All lanes: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 74 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-ACSL6/ACS2 antibody [EPR29174-76] (ab317703), expandable thumbnail

    Western blot - Anti-ACSL6/ACS2 antibody [EPR29174-76] (ab317703)

    This antibody does not cross-react with human ACSL1 and ACSL5.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    In Western blot, Anti-GST antibody [EPR4236] (Anti-GST antibody [EPR4236] ab111947) staining at 1/1000 dilution.

    All lanes: Western blot - Anti-ACSL6/ACS2 antibody [EPR29174-76] (ab317703) at 1/1000 dilution

    Lane 1: His-tagged human ACSL6/ACS2 fragment at 10 ng with NFDM/TBST

    Lane 2: GST-tagged human ACSL5 fragment at 10 ng with NFDM/TBST

    Lane 3: His-tagged human ACSL1 fragment at 10 ng with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 15 kDa, 37 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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