Rabbit Polyclonal ACSS2 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ACSS2.
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Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ACSS2. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NR19
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Catalyzes the synthesis of acetyl-CoA from short-chain fatty acids (PubMed:10843999, PubMed:28003429, PubMed:28552616). Acetate is the preferred substrate (PubMed:10843999, PubMed:28003429). Can also utilize propionate with a much lower affinity (By similarity). Nuclear ACSS2 promotes glucose deprivation-induced lysosomal biogenesis and autophagy, tumor cell survival and brain tumorigenesis (PubMed:28552616). Glucose deprivation results in AMPK-mediated phosphorylation of ACSS2 leading to its translocation to the nucleus where it binds to TFEB and locally produces acetyl-CoA for histone acetylation in the promoter regions of TFEB target genes thereby activating their transcription (PubMed:28552616). The regulation of genes associated with autophagy and lysosomal activity through ACSS2 is important for brain tumorigenesis and tumor survival (PubMed:28552616). Acts as a chromatin-bound transcriptional coactivator that up-regulates histone acetylation and expression of neuronal genes (By similarity). Can be recruited to the loci of memory-related neuronal genes to maintain a local acetyl-CoA pool, providing the substrate for histone acetylation and promoting the expression of specific genes, which is essential for maintaining long-term spatial memory (By similarity).
Alternative names
ACAS2, ACSS2, Acetate--CoA ligase, Acetyl-CoA synthetase, Acetyl-CoA synthetase 1, Acyl-CoA synthetase short-chain family member 2, Acyl-activating enzyme, Propionate--CoA ligase, ACS, AceCS, AceCS1
Recommended products
Rabbit Polyclonal ACSS2 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ACSS2.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ACSS2. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NR19
- Purification technique
- Affinity purification Immunogen
- Specificity
Blast analysis of the sequence of the immunogen shows 100% identity with human and 80% identity to mouse.
- Concentration
- Purification notes
This affinity purified antibody is directed against human ACSS2 protein. Anti-ACSS2 Antibody to was affinity purified from monospecific antiserum by immunoaffinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ACSS2 also known as acyl-CoA synthetase short-chain family member 2 plays an important role in acetate metabolism and energy generation. This enzyme is responsible for the conversion of acetate into acetyl-CoA an essential metabolite in cellular processes. ACSS2 has a molecular mass of approximately 73 kDa and its expression occurs mainly in liver brain and testis tissues highlighting its widespread physiological relevance.
- Biological function summary
ACSS2 regulates cellular energy homeostasis by producing acetyl-CoA for energy production and biosynthetic reactions. This enzyme provides the necessary substrate for the synthesis of fatty acids cholesterol and acetylation of proteins impacting cellular metabolism and function. ACSS2 operates independently and does not form part of any known enzyme complex emphasizing its unique role in acetate utilization.
- Pathways
ACSS2 functions as a central component of lipid metabolism and the acetyl-CoA positioned pathways. It integrates into the glycolysis-associated pathways when it contributes to the energy-producing acetyl-CoA pool. ACSS2 interacts with other metabolic enzymes like ATP citrate lyase in controlling the balance of acetyl-CoA production which is important for both lipid synthesis and energy metabolism.
- Associated diseases and disorders
Abnormalities in ACSS2 activity have links to conditions like cancer and metabolic syndrome. Alterations in the expression or function of ACSS2 can affect energy metabolism contributing to the growth and survival of cancer cells. Additionally the protein's dysregulation might play a role in metabolic syndrome associated with improper lipid and glucose homeostasis. ACSS2 interacts with oncogenes and metabolic regulators such as AMPK emphasizing its relevance in disease modulation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-ACSS2 antibody (ab263966)
Primary incubation overnight at 4°C.
Secondary incubation for 45 minutes at RT.
M: Molecular weight marker.
All lanes: Western blot - Anti-ACSS2 antibody (ab263966) at 1/1000 dilution
All lanes: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg
SecondaryAll lanes: Rabbit secondary HRP at 1/70000 dilution
Predicted band size: 79 kDa
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