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AB264391

Anti-ACSS2 antibody

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Rabbit Polyclonal ACSS2 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ACSS2 aa 450 to C-terminus.

View Alternative Names

ACAS2, ACSS2, Acetate--CoA ligase, Acetyl-CoA synthetase, Acetyl-CoA synthetase 1, Acyl-CoA synthetase short-chain family member 2, Acyl-activating enzyme, Propionate--CoA ligase, ACS, AceCS, AceCS1

1 Images
Western blot - Anti-ACSS2 antibody (AB264391)
  • WB

Supplier Data

Western blot - Anti-ACSS2 antibody (AB264391)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-ACSS2 antibody (ab264391) at 0.1 µg/mL

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 50 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 3:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 4:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 79 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human ACSS2 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NR19

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ACSS2 also known as acyl-CoA synthetase short-chain family member 2 plays an important role in acetate metabolism and energy generation. This enzyme is responsible for the conversion of acetate into acetyl-CoA an essential metabolite in cellular processes. ACSS2 has a molecular mass of approximately 73 kDa and its expression occurs mainly in liver brain and testis tissues highlighting its widespread physiological relevance.
Biological function summary

ACSS2 regulates cellular energy homeostasis by producing acetyl-CoA for energy production and biosynthetic reactions. This enzyme provides the necessary substrate for the synthesis of fatty acids cholesterol and acetylation of proteins impacting cellular metabolism and function. ACSS2 operates independently and does not form part of any known enzyme complex emphasizing its unique role in acetate utilization.

Pathways

ACSS2 functions as a central component of lipid metabolism and the acetyl-CoA positioned pathways. It integrates into the glycolysis-associated pathways when it contributes to the energy-producing acetyl-CoA pool. ACSS2 interacts with other metabolic enzymes like ATP citrate lyase in controlling the balance of acetyl-CoA production which is important for both lipid synthesis and energy metabolism.

Abnormalities in ACSS2 activity have links to conditions like cancer and metabolic syndrome. Alterations in the expression or function of ACSS2 can affect energy metabolism contributing to the growth and survival of cancer cells. Additionally the protein's dysregulation might play a role in metabolic syndrome associated with improper lipid and glucose homeostasis. ACSS2 interacts with oncogenes and metabolic regulators such as AMPK emphasizing its relevance in disease modulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the synthesis of acetyl-CoA from short-chain fatty acids (PubMed : 10843999, PubMed : 28003429, PubMed : 28552616). Acetate is the preferred substrate (PubMed : 10843999, PubMed : 28003429). Can also utilize propionate with a much lower affinity (By similarity). Nuclear ACSS2 promotes glucose deprivation-induced lysosomal biogenesis and autophagy, tumor cell survival and brain tumorigenesis (PubMed : 28552616). Glucose deprivation results in AMPK-mediated phosphorylation of ACSS2 leading to its translocation to the nucleus where it binds to TFEB and locally produces acetyl-CoA for histone acetylation in the promoter regions of TFEB target genes thereby activating their transcription (PubMed : 28552616). The regulation of genes associated with autophagy and lysosomal activity through ACSS2 is important for brain tumorigenesis and tumor survival (PubMed : 28552616). Acts as a chromatin-bound transcriptional coactivator that up-regulates histone acetylation and expression of neuronal genes (By similarity). Can be recruited to the loci of memory-related neuronal genes to maintain a local acetyl-CoA pool, providing the substrate for histone acetylation and promoting the expression of specific genes, which is essential for maintaining long-term spatial memory (By similarity).
See full target information ACSS2
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Product promise

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