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Knockout Tested Rabbit Recombinant Monoclonal ACSS2 antibody. Carrier free. Suitable for WB, IP and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-ACSS2 antibody [EPR27496-4] - BSA and Azide free (AB314491), expandable thumbnail
  • Immunoprecipitation - Anti-ACSS2 antibody [EPR27496-4] - BSA and Azide free (AB314491), expandable thumbnail
  • Western blot - Anti-ACSS2 antibody [EPR27496-4] - BSA and Azide free (AB314491), expandable thumbnail
  • Western blot - Anti-ACSS2 antibody [EPR27496-4] - BSA and Azide free (AB314491), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPIHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Mouse
Tested
Expected
Not recommended
Not recommended
Not recommended
Rat
Tested
Expected
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Catalyzes the synthesis of acetyl-CoA from short-chain fatty acids (PubMed:10843999, PubMed:28003429, PubMed:28552616). Acetate is the preferred substrate (PubMed:10843999, PubMed:28003429). Can also utilize propionate with a much lower affinity (By similarity). Nuclear ACSS2 promotes glucose deprivation-induced lysosomal biogenesis and autophagy, tumor cell survival and brain tumorigenesis (PubMed:28552616). Glucose deprivation results in AMPK-mediated phosphorylation of ACSS2 leading to its translocation to the nucleus where it binds to TFEB and locally produces acetyl-CoA for histone acetylation in the promoter regions of TFEB target genes thereby activating their transcription (PubMed:28552616). The regulation of genes associated with autophagy and lysosomal activity through ACSS2 is important for brain tumorigenesis and tumor survival (PubMed:28552616). Acts as a chromatin-bound transcriptional coactivator that up-regulates histone acetylation and expression of neuronal genes (By similarity). Can be recruited to the loci of memory-related neuronal genes to maintain a local acetyl-CoA pool, providing the substrate for histone acetylation and promoting the expression of specific genes, which is essential for maintaining long-term spatial memory (By similarity).

Alternative names

ACAS2, ACSS2, Acetate--CoA ligase, Acetyl-CoA synthetase, Acetyl-CoA synthetase 1, Acyl-CoA synthetase short-chain family member 2, Acyl-activating enzyme, Propionate--CoA ligase, ACS, AceCS, AceCS1

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal ACSS2 antibody. Carrier free. Suitable for WB, IP and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR27496-4
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab314491 is the carrier-free version of Anti-ACSS2 antibody [EPR27496-4] ab314490.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ACSS2 also known as acyl-CoA synthetase short-chain family member 2 plays an important role in acetate metabolism and energy generation. This enzyme is responsible for the conversion of acetate into acetyl-CoA an essential metabolite in cellular processes. ACSS2 has a molecular mass of approximately 73 kDa and its expression occurs mainly in liver brain and testis tissues highlighting its widespread physiological relevance.

Biological function summary

ACSS2 regulates cellular energy homeostasis by producing acetyl-CoA for energy production and biosynthetic reactions. This enzyme provides the necessary substrate for the synthesis of fatty acids cholesterol and acetylation of proteins impacting cellular metabolism and function. ACSS2 operates independently and does not form part of any known enzyme complex emphasizing its unique role in acetate utilization.

Pathways

ACSS2 functions as a central component of lipid metabolism and the acetyl-CoA positioned pathways. It integrates into the glycolysis-associated pathways when it contributes to the energy-producing acetyl-CoA pool. ACSS2 interacts with other metabolic enzymes like ATP citrate lyase in controlling the balance of acetyl-CoA production which is important for both lipid synthesis and energy metabolism.

Associated diseases and disorders

Abnormalities in ACSS2 activity have links to conditions like cancer and metabolic syndrome. Alterations in the expression or function of ACSS2 can affect energy metabolism contributing to the growth and survival of cancer cells. Additionally the protein's dysregulation might play a role in metabolic syndrome associated with improper lipid and glucose homeostasis. ACSS2 interacts with oncogenes and metabolic regulators such as AMPK emphasizing its relevance in disease modulation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-ACSS2 antibody [EPR27496-4] - BSA and Azide free (ab314491), expandable thumbnail

    Western blot - Anti-ACSS2 antibody [EPR27496-4] - BSA and Azide free (ab314491)

    This data was developed using Anti-ACSS2 antibody [EPR27496-4] ab314490, the same antibody clone in a different buffer formulation.

    Western blot: Rabbit Monoclonal[EPR27496-4] to ACSS2 Anti-ACSS2 antibody [EPR27496-4] ab314490 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 75 kDa in Wild-type U-87 MG ab278079 cell lysates with no signal observed at this size in ACSS2 knockout U-87 MG cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

    All lanes: Western blot - Anti-ACSS2 antibody [EPR27496-4] (Anti-ACSS2 antibody [EPR27496-4] ab314490) at 1/1000 dilution

    Lane 1: Wild-type U-87 MG ab278079 at 20 µg

    Lane 2: ACSS2 knockout U-87 MG at 20 µg

    Lane 3: Wild-type HAP1 at 20 µg

    Lane 4: ACSS2 knockout HAP1 at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 79 kDa

    Observed band size: 75 kDa

  • Immunoprecipitation - Anti-ACSS2 antibody [EPR27496-4] - BSA and Azide free (ab314491), expandable thumbnail

    Immunoprecipitation - Anti-ACSS2 antibody [EPR27496-4] - BSA and Azide free (ab314491)

    This data was developed using Anti-ACSS2 antibody [EPR27496-4] ab314490, the same antibody clone in a different buffer formulation.
    ACSS2 was immunoprecipitated from 0.35 mg HAP1 (human chronic myelogenous leukemia near-haploid cell line) whole cell lysate with Anti-ACSS2 antibody [EPR27496-4] ab314490 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-ACSS2 antibody [EPR27496-4] ab314490 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
    Lane 1: HAP1 (human chronic myelogenous leukemia near-haploid cell line) whole cell lysate
    Lane 2: Anti-ACSS2 antibody [EPR27496-4] ab314490 IP in HAP1 (human chronic myelogenous leukemia near-haploid cell line) whole cell lysate
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-ACSS2 antibody [EPR27496-4] ab314490 in HAP1 whole cell lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-ACSS2 antibody [EPR27496-4] (Anti-ACSS2 antibody [EPR27496-4] ab314490) at 1/30 dilution

    All lanes: HAP1 (human chronic myelogenous leukemia near-haploid cell line) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 8s

  • Western blot - Anti-ACSS2 antibody [EPR27496-4] - BSA and Azide free (ab314491), expandable thumbnail

    Western blot - Anti-ACSS2 antibody [EPR27496-4] - BSA and Azide free (ab314491)

    This data was developed using Anti-ACSS2 antibody [EPR27496-4] ab314490, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: Lane 1: 5 seconds, Lane 2-4: 37 seconds.

    All lanes: Western blot - Anti-ACSS2 antibody [EPR27496-4] (Anti-ACSS2 antibody [EPR27496-4] ab314490) at 1/1000 dilution

    Lane 1: Mouse liver tissue lysate at 50 µg

    Lane 2: Mouse brain tissue lysate at 50 µg

    Lane 3: Rat brain tissue lysate at 50 µg

    Lane 4: Rat liver tissue lysate at 50 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 79 kDa

  • Western blot - Anti-ACSS2 antibody [EPR27496-4] - BSA and Azide free (ab314491), expandable thumbnail

    Western blot - Anti-ACSS2 antibody [EPR27496-4] - BSA and Azide free (ab314491)

    This data was developed using Anti-ACSS2 antibody [EPR27496-4] ab314490, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Performed under reducing conditions.

    In Western blot, Anti-ACSS2 antibody [EPR27496-4] ab314490 was shown to bind specifically to ACSS2. Target of interest was observed at 79 kDa in wild-type HAP1 cell lysates (lane 1) with no signal observed at this size in ACSS2 knockout cell line (lane 2).
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-ACSS2 antibody [EPR27496-4] (Anti-ACSS2 antibody [EPR27496-4] ab314490) at 1/1000 dilution

    Lane 1: Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell line) whole cell lysate at 50 µg

    Lane 2: ACSS2 knockout HAP1 whole cell lysate at 50 µg

    Lane 3: U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 50 µg

    Lane 4: L6 (rat skeletal muscle myoblast) whole cell lysate at 50 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Performed under reducing conditions.

    Observed band size: 79 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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