AB97396

Anti-ACT antibody

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(3 Publications)

Rabbit Polyclonal ACT antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FHL5 aa 1-200.

View Alternative Names

ACT, FHL5, Four and a half LIM domains protein 5, FHL-5, Activator of cAMP-responsive element modulator in testis, Activator of CREM in testis

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACT antibody (AB97396)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACT antibody (AB97396)

ab97396 at 1/500 dilution, staining human ACT in SW480 xenograft - Formalin/PFA-fixed paraffin-embedded tissue.

Unknown

Western blot - Anti-ACT antibody (AB97396)

Gel conc 12 % (SDS PAGE)

All lanes:

Western blot - Anti-ACT antibody (ab97396) at 1/1000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

Raji whole cell lysate at 30 µg

Predicted band size: 33 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human FHL5 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q5TD97

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ACT also known as Alpha-1-antichymotrypsin is a serine protease inhibitor primarily involved in inhibiting enzymes like cathepsin G and chymase. It has a molecular mass of approximately 68 kDa. This protein is mainly expressed in hepatocytes but can also be found in other tissues including the lungs and intestines. Its role in the immune response and inflammation is significant given its protease inhibitory function.

Biological function summary

Alpha-1-antichymotrypsin plays a critical role in controlling proteolytic activities to prevent tissue damage during inflammation. As part of a family of serine protease inhibitors it forms complexes with its target enzymes thereby regulating their activity. This regulation is vital for maintaining homeostasis in bodily tissues especially during acute phase responses in the liver.

Pathways

Alpha-1-antichymotrypsin is involved in the acute phase response pathway which is key in modulating inflammation and responding to tissue injury. It also participates in the complement and coagulation cascades. Proteins such as C-reactive protein and other serine protease inhibitors are linked through these pathways working together to manage the body's response to damage and infection.

Abnormalities in Alpha-1-antichymotrypsin function have been linked to chronic obstructive pulmonary disease (COPD) and Alzheimer's disease. Deficiencies or mutations in this protein can exacerbate the protease-antiprotease imbalance in COPD. In Alzheimer's disease ACT is known to associate with amyloid plaques possibly influencing their formation or stability. Other proteins like the amyloid-beta peptide are connected through these diseases highlighting the complex interplay between ACT and pathological processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May be involved in the regulation of spermatogenesis. Stimulates CREM transcriptional activity in a phosphorylation-independent manner.

See full target information FHL5

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Infection and immunity 91:e0023623 PubMed37861311

2023

Polyamines and hypusination are important for toxin B (TcdB)-mediated activation of group 3 innate lymphocytes (ILC3s).

Applications

Unspecified application

Species

Unspecified reactive species

Prakash Sah,Bailey A Knighten,Megan A Reidy,Lauren A Zenewicz

The New phytologist 240:1134-1148 PubMed37606093

2023

Orosomucoid proteins limit endoplasmic reticulum stress in plants.

Applications

Unspecified application

Species

Unspecified reactive species

Ling-Yan Wang,Jian Li,Benqiang Gong,Rui-Hua Wang,Yi-Li Chen,Jian Yin,Chang Yang,Jia-Ting Lin,Hao-Zhuo Liu,Yubing Yang,Jianfeng Li,Chunyu Li,Nan Yao

Cell journal 23:736-741 PubMed34979062

2021

Dynamic Expression and Chromatin Incorporation of ACT and CREM Transcription Factors in Testis Tissues of Infertile Men.

Applications

Unspecified application

Species

Unspecified reactive species

Parham Jazireian,Raha Favaedi,Mohammad Ali Sadighi Gilani,Maryam Shahhoseini

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ACT antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACT antibody (AB97396)

Western blot - Anti-ACT antibody (AB97396)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise