AB151787

Anti-ACTC1 antibody

Name: Anti-ACTC1 antibody (ab151787) | Abcam
Brand: Abcam Limited
SKU: AB151787

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(3 Publications)

Rabbit Polyclonal ACTC antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Actc1 aa 100 to C-terminus.

View Alternative Names

Actc, Actc1, Alpha-cardiac actin

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTC1 antibody (AB151787)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTC1 antibody (AB151787)

Immunohistochemical analysis of paraffin-embedded mouse muscle tissue labelling alpha Cardiac Muscle Actin protein at cytoplasm with ab151787 at a 1/500 dilution.
Antigen retrieval : EDTA based, pH 8.0 buffer, 15min

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTC1 antibody (AB151787)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTC1 antibody (AB151787)

Immunohistochemical analysis of paraffin-embedded mouse heart tissue labelling alpha Cardiac Muscle Actin protein at cytoplasm with ab151787 at a 1/500 dilution.
Antigen retrieval : EDTA based, pH 8.0 buffer, 15min

Unknown

Western blot - Anti-ACTC1 antibody (AB151787)

10% SDS PAGE

All lanes:

Western blot - Anti-ACTC1 antibody (ab151787) at 1/1000 dilution

All lanes:

NIH3T3 whole cell lysate at 30 µg

Predicted band size: 42 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Mouse Actc1 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P68033

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ACTC1 also known as cardiac alpha actin is a protein that plays an essential role in the cytoskeletal structure of heart muscle cells. Weighing around 42 kDa ACTC1 is found predominantly in cardiac tissue specifically in the sarcomere which is the fundamental unit of muscle contraction. In muscle cells ACTC1 organizes into thin filaments and is critical for maintaining the structural integrity and contractile function of the cardiac tissue.

Biological function summary

ACTC1 is significant in muscle contraction and cellular movement and it forms part of the sarcomeric complex within cardiac muscle cells. This protein along with other actin isoforms contributes to the organized network of actin and myosin filaments which generate force for heartbeats. ACTC1 polymerizes to form the actin filament which when coupled with myosin motors and regulatory proteins executes the contraction cycle in cardiac muscles.

Pathways

ACTC1 plays a central role within the muscle contraction process and the cardiac muscle contraction pathway. In these pathways its interaction with proteins like myosin tropomyosin and troponin is vital to initiate and regulate cardiac contractions. The dynamic interaction between ACTC1 and these proteins facilitates the conversion of chemical energy into mechanical energy necessary for the rhythmic contraction and relaxation of heart muscles.

Mutations or alterations in ACTC1 are closely linked to cardiac conditions such as hypertrophic cardiomyopathy and dilated cardiomyopathy. These disorders often involve associated proteins like troponin T and myosin heavy chain which when mutated along with ACTC1 disrupt normal cardiac contraction and lead to structural heart changes. These conditions highlight the importance of ACTC1 in maintaining normal cardiac function and structure.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

See full target information Actc1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 38:e23390 PubMed38169064

2024

Single-cell RNA sequencing reveals the heterogeneity of epithelial cell and fibroblast cells from non- to metastatic lymph node OTSCC.

Applications

Unspecified application

Species

Unspecified reactive species

Dandan Song,Fei Yang,Yang Sun,Xingwen Wu,Qianrong Zhou,Wei Bi,Jian Sun,Siyi Li,Youcheng Yu

Nucleus (Austin, Tex.) 6:490-506 PubMed26652669

2015

Rapamycin reduces fibroblast proliferation without causing quiescence and induces STAT5A/B-mediated cytokine production.

Applications

Unspecified application

Species

Unspecified reactive species

Zoe E Gillespie,Kimberly MacKay,Michelle Sander,Brett Trost,Wojciech Dawicki,Aruna Wickramarathna,John Gordon,Mark Eramian,Ian R Kill,Joanna M Bridger,Anthony Kusalik,Jennifer A Mitchell,Christopher H Eskiw

American journal of physiology. Endocrinology and 305:E325-35 PubMed23736538

2013

Activation of the amino acid response modulates lineage specification during differentiation of murine embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jixiu Shan,Takashi Hamazaki,Tiffany A Tang,Naohiro Terada,Michael S Kilberg

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ACTC1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTC1 antibody (AB151787)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTC1 antibody (AB151787)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTC1 antibody (AB151787)

Western blot - Anti-ACTC1 antibody (AB151787)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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