JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB239475

Anti-ACTC1 antibody [C3]

Be the first to review this product! Submit a review

|

(1 Publication)

Mouse Monoclonal ACTC antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ACTC1 aa 150 to C-terminus.

View Alternative Names

ACTC, ACTC1, Alpha-cardiac actin

2 Images
Western blot - Anti-ACTC1 antibody [C3] (AB239475)
  • WB

Supplier Data

Western blot - Anti-ACTC1 antibody [C3] (AB239475)

All lanes:

Western blot - Anti-ACTC1 antibody [C3] (ab239475) at 3 µg/mL

Lane 1:

Mouse heart lysate

Lane 2:

Mouse skeletal muscle lysate

Lane 3:

Mouse skin lysate

Secondary

All lanes:

HRP-Linked Rabbit Anti-Mouse IgG at 1/5000 dilution

Predicted band size: 42 kDa

false

Western blot - Anti-ACTC1 antibody [C3] (AB239475)
  • WB

Supplier Data

Western blot - Anti-ACTC1 antibody [C3] (AB239475)

All lanes:

Western blot - Anti-ACTC1 antibody [C3] (ab239475) at 3 µg/mL

All lanes:

Recombinant human ACTC1 protein

Secondary

All lanes:

HRP-Linked Rabbit Anti-Mouse IgG at 1/5000 dilution

Predicted band size: 42 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

C3

Isotype

IgG2b

Light chain type

kappa

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human ACTC1 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P68032

style
left-column

Alternative Products

Primary Antibodies

AB151787

Anti-ACTC1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTC1 antibody (AB151787)

  • 0
  • 0
Primary Antibodies

AB218549

Anti-ACTC1 antibody [RM257] - N-terminal

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTC1 antibody [RM257] - N-terminal (AB218549)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB156302

Anti-muscle Actin antibody [EPR8484] - Loading Control

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [EPR8484] - Loading Control (AB156302)

  • 2
  • 1
Primary Antibodies

AB207926

Anti-MYH6 antibody [3-48 G5C7]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH6 antibody [3-48 G5C7] (AB207926)

  • 0
  • 0
Primary Antibodies

AB179467

Anti-Actin antibody [EPR16769] - Loading Control

20ul selling size|RabMAb|Recombinant|Lab Essentials

Flow Cytometry (Intracellular) - Anti-Actin antibody [EPR16769] - Loading Control (AB179467)

  • 5
  • 11
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS.
Storage buffer
pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ACTC1 also known as cardiac alpha actin is a protein that plays an essential role in the cytoskeletal structure of heart muscle cells. Weighing around 42 kDa ACTC1 is found predominantly in cardiac tissue specifically in the sarcomere which is the fundamental unit of muscle contraction. In muscle cells ACTC1 organizes into thin filaments and is critical for maintaining the structural integrity and contractile function of the cardiac tissue.
Biological function summary

ACTC1 is significant in muscle contraction and cellular movement and it forms part of the sarcomeric complex within cardiac muscle cells. This protein along with other actin isoforms contributes to the organized network of actin and myosin filaments which generate force for heartbeats. ACTC1 polymerizes to form the actin filament which when coupled with myosin motors and regulatory proteins executes the contraction cycle in cardiac muscles.

Pathways

ACTC1 plays a central role within the muscle contraction process and the cardiac muscle contraction pathway. In these pathways its interaction with proteins like myosin tropomyosin and troponin is vital to initiate and regulate cardiac contractions. The dynamic interaction between ACTC1 and these proteins facilitates the conversion of chemical energy into mechanical energy necessary for the rhythmic contraction and relaxation of heart muscles.

Mutations or alterations in ACTC1 are closely linked to cardiac conditions such as hypertrophic cardiomyopathy and dilated cardiomyopathy. These disorders often involve associated proteins like troponin T and myosin heavy chain which when mutated along with ACTC1 disrupt normal cardiac contraction and lead to structural heart changes. These conditions highlight the importance of ACTC1 in maintaining normal cardiac function and structure.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
See full target information ACTC1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Respiratory research 24:83 PubMed36927357

2023

Proteome of airway surface liquid and mucus in newborn wildtype and cystic fibrosis piglets.

Applications

Unspecified application

Species

Unspecified reactive species

Ana M Rodriguez-Piñeiro,Florian Jaudas,Nikolai Klymiuk,Andrea Bähr,Gunnar C Hansson,Anna Ermund
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com