AB46805

Anti-ACTC1 antibody [EP184E]

RabMAb
Recombinant
What is this?

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(25 Publications)

Rabbit Recombinant Monoclonal ACTC antibody. Suitable for IHC-P, WB, IHC-FoFr, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 25 publications.

View Alternative Names

ACTC, ACTC1, Alpha-cardiac actin

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTC1 antibody [EP184E] (AB46805)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTC1 antibody [EP184E] (AB46805)

Immunohistochemical staining of paraffin-embedded human cardiac muscle using anti-Actin ab46805 at a 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-ACTC1 antibody [EP184E] (AB46805)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ACTC1 antibody [EP184E] (AB46805)

Immunofluorescent staining of HeLa cells using ab46805 at a 1/250 dilution.

Flow Cytometry (Intracellular) - Anti-ACTC1 antibody [EP184E] (AB46805)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-ACTC1 antibody [EP184E] (AB46805)

Overlay histogram showing HeLa cells stained with ab46805 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab46805 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTC1 antibody [EP184E] (AB46805)

Fluorescent immunohistochemical analysis of paraffin-embedded human smooth muscle tissue using ab46805. Green-specific fluorescent staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-ACTC1 antibody [EP184E] (AB46805)

All lanes:

Western blot - Anti-ACTC1 antibody [EP184E] (ab46805) at 1/1000 dilution

All lanes:

A431 cell lysate at 10ug/lane

Secondary

All lanes:

Goat anti-rabbit, HRP labelled. at 1/2000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

false

Carrier free

Anti-ACTC1 antibody [EP184E] - BSA and Azide free
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-ACTC1 antibody [EP184E]
HRP

HRP Anti-ACTC1 antibody [EP184E]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP184E

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-FoFr, ICC/IF, Flow Cyt (Intra), WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This Actin antibody recognizes all muscle actin.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/200", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFoFr-species-checked": "predicted", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFoFr-species-checked": "predicted", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFoFr-species-checked": "predicted", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ACTC1 also known as cardiac alpha actin is a protein that plays an essential role in the cytoskeletal structure of heart muscle cells. Weighing around 42 kDa ACTC1 is found predominantly in cardiac tissue specifically in the sarcomere which is the fundamental unit of muscle contraction. In muscle cells ACTC1 organizes into thin filaments and is critical for maintaining the structural integrity and contractile function of the cardiac tissue.

Biological function summary

ACTC1 is significant in muscle contraction and cellular movement and it forms part of the sarcomeric complex within cardiac muscle cells. This protein along with other actin isoforms contributes to the organized network of actin and myosin filaments which generate force for heartbeats. ACTC1 polymerizes to form the actin filament which when coupled with myosin motors and regulatory proteins executes the contraction cycle in cardiac muscles.

Pathways

ACTC1 plays a central role within the muscle contraction process and the cardiac muscle contraction pathway. In these pathways its interaction with proteins like myosin tropomyosin and troponin is vital to initiate and regulate cardiac contractions. The dynamic interaction between ACTC1 and these proteins facilitates the conversion of chemical energy into mechanical energy necessary for the rhythmic contraction and relaxation of heart muscles.

Mutations or alterations in ACTC1 are closely linked to cardiac conditions such as hypertrophic cardiomyopathy and dilated cardiomyopathy. These disorders often involve associated proteins like troponin T and myosin heavy chain which when mutated along with ACTC1 disrupt normal cardiac contraction and lead to structural heart changes. These conditions highlight the importance of ACTC1 in maintaining normal cardiac function and structure.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

See full target information ACTC1

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Publications (25)

Recent publications for all applications. Explore the full list and refine your search

Circulation. Heart failure 17:e011504 PubMed38910562

2024

Overexpression of in Cardiomyocytes Promotes Cardiac Reverse Remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Mengda Xu,Hang Zhang,Yuan Chang,Xiumeng Hua,Xiao Chen,Yixuan Sheng,Dan Shan,Mengni Bao,Shengshou Hu,Jiangping Song

Journal of dairy science 106:9807-9821 PubMed37641328

2023

Plant polyphenol extract supplementation affects performance, welfare, and the Nrf2-oxidative stress response in adipose tissue of heat-stressed dairy cows.

Applications

Unspecified application

Species

Unspecified reactive species

J R Daddam,D Daniel,G Kra,I Pelech,Y Portnick,U Moallem,Y Lavon,M Zachut

Scientific reports 13:5280 PubMed37002295

2023

Alpha-linolenic acid modulates systemic and adipose tissue-specific insulin sensitivity, inflammation, and the endocannabinoid system in dairy cows.

Applications

Unspecified application

Species

Unspecified reactive species

Gitit Kra,Jayasimha Rayalu Daddam,Uzi Moallem,Hadar Kamer,Batel Mualem,Yishai Levin,Radka Kočvarová,Alina Nemirovski,Andres G Contreras,Joseph Tam,Maya Zachut

Journal of animal science and biotechnology 13:114 PubMed36183098

2022

Effects of omega-3 supplementation on components of the endocannabinoid system and metabolic and inflammatory responses in adipose and liver of peripartum dairy cows.

Applications

Unspecified application

Species

Unspecified reactive species

Gitit Kra,Jayasimha Rayalu Daddam,Uzi Moallem,Hadar Kamer,Radka Kočvarová,Alina Nemirovski,G Andres Contreras,Joseph Tam,Maya Zachut

Animals : an open access journal from MDPI 12: PubMed35327191

2022

Effects of Environmental Heat Load on Endocannabinoid System Components in Adipose Tissue of High Yielding Dairy Cows.

Applications

Unspecified application

Species

Unspecified reactive species

Gitit Kra,Jayasimha Rayalu Daddam,Uzi Moallem,Hadar Kamer,Majdoleen Ahmad,Alina Nemirovski,G Andres Contreras,Joseph Tam,Maya Zachut

International journal of molecular sciences 22: PubMed33810070

2021

Phosphoproteomic Analysis of Subcutaneous and Omental Adipose Tissue Reveals Increased Lipid Turnover in Dairy Cows Supplemented with Conjugated Linoleic Acid.

Applications

Unspecified application

Species

Unspecified reactive species

Jayasimha Rayalu Daddam,Harald M Hammon,Arnulf Tröscher,Laura Vogel,Martina Gnott,Gitit Kra,Yishai Levin,Helga Sauerwein,Maya Zachut

Journal of dairy science 103:10809-10822 PubMed32896401

2020

Seasonal heat load is more potent than the degree of body weight loss in dysregulating immune function by reducing white blood cell populations and increasing inflammation in Holstein dairy cows.

Applications

Unspecified application

Species

Unspecified reactive species

M Zachut,G Kra,N Nemes-Navon,N Ben-Aharon,U Moallem,Y Lavon,S Jacoby

Communications biology 3:330 PubMed32587337

2020

Pre-innervated tissue-engineered muscle promotes a pro-regenerative microenvironment following volumetric muscle loss.

Applications

Unspecified application

Species

Unspecified reactive species

Suradip Das,Kevin D Browne,Franco A Laimo,Joseph C Maggiore,Melanie C Hilman,Halimulati Kaisaier,Carlos A Aguilar,Zarina S Ali,Foteini Mourkioti,D Kacy Cullen

Cell metabolism 30:1107-1119.e8 PubMed31607564

2019

A Novel Allosteric Inhibitor of Phosphoglycerate Mutase 1 Suppresses Growth and Metastasis of Non-Small-Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Huang,Qian Liang,Ye Zhou,Lu-Lu Jiang,Wei-Ming Gu,Ming-Yu Luo,Ya-Bin Tang,Yang Wang,Wei Lu,Min Huang,Sheng-Zhe Zhang,Guang-Lei Zhuang,Qing Dai,Qian-Cheng Shen,Jian Zhang,Hui-Min Lei,Liang Zhu,De-Yong Ye,Hong-Zhuan Chen,Lu Zhou,Ying Shen

Journal of proteomics 204:103399 PubMed31152939

2019

Proteomic analysis reveals greater abundance of complement and inflammatory proteins in subcutaneous adipose tissue from postpartum cows treated with sodium salicylate.

Applications

Unspecified application

Species

Unspecified reactive species

C S Takiya,S R Montgomery,L K Mamedova,G Kra,N Nemes-Navon,Y Levin,S D Fleming,B J Bradford,M Zachut

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ACTC1 antibody [EP184E]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTC1 antibody [EP184E] (AB46805)

Immunocytochemistry/ Immunofluorescence - Anti-ACTC1 antibody [EP184E] (AB46805)

Flow Cytometry (Intracellular) - Anti-ACTC1 antibody [EP184E] (AB46805)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTC1 antibody [EP184E] (AB46805)

Western blot - Anti-ACTC1 antibody [EP184E] (AB46805)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Secondary Antibodies

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (25)

Product promise