AB231802

Anti-ACTG2 antibody

Name: Anti-ACTG2 antibody (ab231802) | Abcam
Brand: Abcam Limited
SKU: AB231802
Price: 525 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(5 Publications)

Rabbit Polyclonal ACTH antibody. Suitable for WB, IHC-P and reacts with Human, Recombinant full length protein - Human, Rat, Pig samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ACTG2.

View Alternative Names

ACTA3, ACTL3, ACTSG, ACTG2, Alpha-actin-3, Gamma-2-actin, Smooth muscle gamma-actin

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTG2 antibody (AB231802)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTG2 antibody (AB231802)

Paraffin-embedded human prostate tissue stained for ACTG2 using ab231802 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTG2 antibody (AB231802)

Paraffin-embedded human liver tissue stained for ACTG2 using ab231802 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTG2 antibody (AB231802)

Paraffin-embedded human lung tissue stained for ACTG2 using ab231802 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTG2 antibody (AB231802)

Paraffin-embedded human prostate cancer tissue stained for ACTG2 using ab231802 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTG2 antibody (AB231802)

Paraffin-embedded human colorectal cancer tissue stained for ACTG2 using ab231802 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTG2 antibody (AB231802)

Paraffin-embedded human stomach tissue stained for ACTG2 using ab231802 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-ACTG2 antibody (AB231802)

All lanes:

Western blot - Anti-ACTG2 antibody (ab231802) at 2 µg/mL

Lane 1:

Human lung lysate

Lane 2:

Pig stomach lysate

Lane 3:

Rat stomach lysate

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

Predicted band size: 42 kDa

false

Supplier Data

Western blot - Anti-ACTG2 antibody (AB231802)

All lanes:

Western blot - Anti-ACTG2 antibody (ab231802) at 2 µg/mL

All lanes:

Recombinant human ACTG2 protein

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

Predicted band size: 42 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human, Pig

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ACTG2.

P63267

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab231802 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ACTG2 protein also known as gamma-2 actin plays a role in the mechanical structure and function of muscle tissues. It is a part of the actin family of proteins which are essential components of the cytoskeleton. ACTG2 has a molecular mass of approximately 42 kDa. This protein is expressed mainly in smooth muscles where it contributes to the structural integrity and contractile function by forming actin filaments.

Biological function summary

The ACTG2 protein is critical in maintaining and regulating the contractility of smooth muscle tissues. It does so by being part of the thin filament complex that interacts with myosin for muscle contraction. This complex is essential for the normal functioning of various organs such as blood vessels gastrointestinal tract and respiratory tract which require smooth muscle contraction for their operation.

Pathways

ACTG2 is integral to the contractile mechanism pathways of smooth muscle cells. It participates in the actin-myosin interaction that converts chemical energy into mechanical work a process essential for muscle contraction in smooth muscle tissue. This actin protein relates to other contractile proteins like myosin heavy chain and tropomyosin which are important within these pathways for effective muscle function.

ACTG2 mutations have connections to visceral myopathy and other types of smooth muscle dysfunctions. Visceral myopathy affects the smooth muscle tissues of the gastrointestinal tract leading to issues like chronic intestinal pseudo-obstruction. In this context ACTG2 mutations can affect interactions with proteins like myosin disrupting contractility and resulting in such disorders.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

See full target information ACTG2

style

left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 28:515 PubMed39247492

2024

Analysis of metastasis‑related risk factors and clinical relevance in adult soft‑tissue sarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Han,Xin Song,Jialiang Liu,Jingfen Zhou,Zhipeng Wu,Haihan Song,Jun Tao,Jian Wang

NPJ Regenerative medicine 8:26 PubMed37236990

2023

Pluripotent stem cell-derived committed cardiac progenitors remuscularize damaged ischemic hearts and improve their function in pigs.

Applications

Unspecified application

Species

Unspecified reactive species

Lynn Yap,Li Yen Chong,Clarissa Tan,Swarnaseetha Adusumalli,Millie Seow,Jing Guo,Zuhua Cai,Sze Jie Loo,Eric Lim,Ru San Tan,Elina Grishina,Poh Loong Soong,Narayan Lath,Lei Ye,Enrico Petretto,Karl Tryggvason

Biomaterials advances 148:213355 PubMed36893487

2023

Patient's dermal fibroblasts as disease markers for visceral myopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Federica Viti,Francesca Micaela Pramotton,Michela Martufi,Raffaella Magrassi,Nicoletta Pedemonte,Mario Nizzari,Francesca Cella Zanacchi,Benedetta De Michele,Manuela Alampi,Martina Zambito,Giuseppe Santamaria,Adriana Bajetto,Sabah Sardar,Valeria Tomati,Paolo Gandullia,Costanza Giampietro,Tullio Florio,Francesco Beltrame,Massimo Vassalli,Isabella Ceccherini

Frontiers in pharmacology 13:1088732 PubMed36686667

2023

Comprehensive analysis of hypoxia-related genes for prognosis value, immune status, and therapy in osteosarcoma patients.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Han,Zhouwei Wu,Zhe Zhang,Jinghao Liang,Chuanpeng Xia,Hede Yan

Frontiers in cell and developmental biology 10:1044198 PubMed36420138

2022

The diversity of trophoblast cells and niches of placenta accreta spectrum disorders revealed by single-cell RNA sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Jingmei Ma,Yawei Liu,Zhirong Guo,Run Sun,Xinrui Yang,Weiran Zheng,Yongdan Ma,Yin Rong,Hongmei Wang,Huixia Yang,Zhenyu Xiao

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ACTG2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTG2 antibody (AB231802)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTG2 antibody (AB231802)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTG2 antibody (AB231802)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTG2 antibody (AB231802)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTG2 antibody (AB231802)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTG2 antibody (AB231802)

Western blot - Anti-ACTG2 antibody (AB231802)

Western blot - Anti-ACTG2 antibody (AB231802)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise