AB189385

Anti-ACTG2 antibody - N-terminal

Name: Anti-ACTG2 antibody - N-terminal (ab189385) | Abcam
Brand: Abcam Limited
SKU: AB189385
Price: 630 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal ACTH antibody. N-terminal. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human ACTG2 aa 1-50.

View Alternative Names

ACTA3, ACTL3, ACTSG, ACTG2, Alpha-actin-3, Gamma-2-actin, Smooth muscle gamma-actin

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTG2 antibody - N-terminal (AB189385)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTG2 antibody - N-terminal (AB189385)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human uterus tissue sections labeling ACTG2 using ab189385 at 5 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTG2 antibody - N-terminal (AB189385)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human prostate tissue sections labeling ACTG2 using ab189385 at 5 μg/ml.

Supplier Data

Western blot - Anti-ACTG2 antibody - N-terminal (AB189385)

All lanes:

Western blot - Anti-ACTG2 antibody - N-terminal (ab189385) at 1/500 dilution

Lane 1:

Extracts from COLO205 cells

Lane 2:

Extracts from COLO205 cells treated with the synthesized peptide

Predicted band size: 42 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human ACTG2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P63267

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ACTG2 protein also known as gamma-2 actin plays a role in the mechanical structure and function of muscle tissues. It is a part of the actin family of proteins which are essential components of the cytoskeleton. ACTG2 has a molecular mass of approximately 42 kDa. This protein is expressed mainly in smooth muscles where it contributes to the structural integrity and contractile function by forming actin filaments.

Biological function summary

The ACTG2 protein is critical in maintaining and regulating the contractility of smooth muscle tissues. It does so by being part of the thin filament complex that interacts with myosin for muscle contraction. This complex is essential for the normal functioning of various organs such as blood vessels gastrointestinal tract and respiratory tract which require smooth muscle contraction for their operation.

Pathways

ACTG2 is integral to the contractile mechanism pathways of smooth muscle cells. It participates in the actin-myosin interaction that converts chemical energy into mechanical work a process essential for muscle contraction in smooth muscle tissue. This actin protein relates to other contractile proteins like myosin heavy chain and tropomyosin which are important within these pathways for effective muscle function.

ACTG2 mutations have connections to visceral myopathy and other types of smooth muscle dysfunctions. Visceral myopathy affects the smooth muscle tissues of the gastrointestinal tract leading to issues like chronic intestinal pseudo-obstruction. In this context ACTG2 mutations can affect interactions with proteins like myosin disrupting contractility and resulting in such disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

See full target information ACTG2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Histochemistry and cell biology 163:93 PubMed40971055

2025

Gamma smooth muscle actin as a new potential marker of cancer-associated fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Michal Španko,Lucie Pfeiferová,Eliška Drobná Krejčí,Michal Kolář,Pavel Dundr,Jaroslav Valach,Karel Smetana,Lukáš Lacina

International neurourology journal 24:29-40 PubMed32252184

2020

Pioglitazone Alters the Proteomes of Normal Bladder Epithelial Cells but Shows No Tumorigenic Effects.

Applications

Unspecified application

Species

Unspecified reactive species

Muhammad Shahid,Minhyung Kim,Austin Yeon,Peng Jin,Woong-Ki Kim,Sungyong You,Jayoung Kim

Proteomics 18:e1800190 PubMed30232827

2018

Quantitative Proteomic Analysis Reveals Caffeine-Perturbed Proteomic Profiles in Normal Bladder Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Muhammad Shahid,Minhyung Kim,Austin Yeon,Allen M Andres,Sungyong You,Jayoung Kim

PloS one 9:e89219 PubMed24586606

2014

Over-expressed Testis-specific Protein Y-encoded 1 as a novel biomarker for male hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shan Li,Cuiju Mo,Shan Huang,Shi Yang,Yu Lu,Qiliu Peng,Jian Wang,Yan Deng,Xue Qin,Yinkun Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ACTG2 antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTG2 antibody - N-terminal (AB189385)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTG2 antibody - N-terminal (AB189385)

Western blot - Anti-ACTG2 antibody - N-terminal (AB189385)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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