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AB74976

Anti-ACTH antibody

4

(3 Reviews)

|

(29 Publications)

Rabbit Polyclonal POMC antibody. Suitable for IHC-P and reacts with Mouse, Human, Rat samples. Cited in 29 publications. Immunogen corresponding to Synthetic Peptide within Human POMC aa 1-50.

View Alternative Names

Pro-opiomelanocortin, POMC, Corticotropin-lipotropin

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTH antibody (AB74976)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTH antibody (AB74976)

Formalin-fixed, paraffin-embedded mouse brain tissue stained for ACTH with ab74976 (1/100 dilution) in immunohistochemical analysis. DAB (brown) and Hematoxylin (blue)staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTH antibody (AB74976)
  • IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTH antibody (AB74976)

ab74976 staining ACTH in P0 Pitx2-Cre/Dicer1 mutant mice pituitary tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Samples were fixed in 4% paraformaldehyde, dehydrated, and embedded in paraffin wax. Sections were cut (7 μm) and stained with H&E. Immunohistochemistry was performed on 7-μm paraffin sections stained by an indirect immunoperoxidase method. Peroxidase activity was visualized with AEC stain kit. ab74976 diluted 1/200. Secondary antibodies conjugated with biotin were used at 1/150 dilution.

Image from Zhang Z et al, J Biol Chem. 2010 Nov 5;285(45):34718-28. Epub 2010 Aug 31, Fig 4. DOI 10.1074/jbc.M110.126441

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human POMC aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P01189

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Goat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Macaque monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Sheep": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.02% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Adrenocorticotropic hormone (ACTH) also known as corticotropin is a peptide hormone secreted by the pituitary gland. It is a 39-amino acid polypeptide with a molecular mass of approximately 4540 Da. ACTH is primarily expressed in the anterior pituitary gland and smaller amounts can be found in the central nervous system. Its production and secretion are tightly regulated by corticotropin-releasing hormone (CRH) through a feedback mechanism involving the hypothalamic-pituitary-adrenal (HPA) axis.
Biological function summary

Adrenocorticotropic hormone stimulates the adrenal cortex to produce and release glucocorticoids especially cortisol. ACTH is not part of a larger complex but acts as a singular hormone in its pathway. The hormone also influences the expression of certain enzymes important for steroid biosynthesis. In addition to its role in the adrenal cortex ACTH can mildly interact with melanocortin receptors on adipose tissues contributing to lipolysis and inflammatory processes.

Pathways

Adrenocorticotropic hormone functions in the HPA axis one of the body's key stress response systems. The hormone also involves in the regulation of the corticosteroid biosynthesis pathway. The primary protein it interacts with in these processes is cortisol which in turn regulates many physiological processes including immune response and metabolism. ACTH and cortisol form a feedback loop where elevated cortisol levels inhibit the release of both CRH and ACTH.

Increased or decreased levels of adrenocorticotropic hormone can result in disorders such as Cushing's disease and Addison's disease. Cushing's disease is characterized by overproduction of ACTH often due to pituitary adenomas leading to excess cortisol production. Conversely in Addison’s disease inadequate ACTH or adrenal cortex dysfunction leads to insufficient cortisol production. Both conditions highlight the disrupted feedback relationship between ACTH and cortisol resulting in significant clinical symptoms that require careful management.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Corticotropin. Stimulates the adrenal glands to release cortisol.. Melanocyte-stimulating hormone alpha. Anorexigenic peptide. Increases the pigmentation of skin by increasing melanin production in melanocytes.. Melanocyte-stimulating hormone beta. Increases the pigmentation of skin by increasing melanin production in melanocytes.. Beta-endorphin. Endogenous orexigenic opiate.. Met-enkephalin. Endogenous opiate.
See full target information POMC

Additional targets

ACTH
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Publications (29)

Recent publications for all applications. Explore the full list and refine your search

Endocrinology, diabetes & metabolism case reports 2024: PubMed39299274

2024

Acquired isolated ACTH deficiency co-occurrence with breast cancer irrespective of paraneoplastic syndrome: coincidence or inevitability.

Applications

Unspecified application

Species

Unspecified reactive species

Shin Urai,Hironori Bando,Mei Nakatsuji,Masaaki Yamamoto,Hidenori Fukuoka,Genzo Iguchi,Wataru Ogawa

Journal of cellular physiology 239:e31428 PubMed39238189

2024

Mineralocorticoid receptor activates postnatal adiposity in zebrafish lacking proopiomelanocortin.

Applications

Unspecified application

Species

Unspecified reactive species

Jithine J Rajeswari,Erin Faught,Helio Santos,Mathilakath M Vijayan

Frontiers in immunology 14:1284301 PubMed38035072

2023

Paraneoplastic isolated adrenocorticotropic hormone deficiency revealed after immune checkpoint inhibitors therapy: new insights into anti-corticotroph antibody.

Applications

Unspecified application

Species

Unspecified reactive species

Shin Urai,Miki Watanabe,Hironori Bando,Yuma Motomura,Masaaki Yamamoto,Motoko Tachihara,Maki Kanzawa,Hidenori Fukuoka,Genzo Iguchi,Wataru Ogawa

Genes 13: PubMed36292565

2022

Characterization of CRH-Binding Protein (CRHBP) in Chickens: Molecular Cloning, Tissue Distribution and Investigation of Its Role as a Negative Feedback Regulator within the Hypothalamus-Pituitary-Adrenal Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yiping Wan,Zheng Zhang,Dongliang Lin,Xinglong Wang,Tianjiao Huang,Jiancheng Su,Jiannan Zhang,Juan Li,Yajun Wang

Endocrinology 163: PubMed35583189

2022

Evidence for Neuropeptide W Acting as a Physiological Corticotropin-releasing Inhibitory Factor in Male Chickens.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Liu,Guixian Bu,Yiping Wan,Jiannan Zhang,Chunheng Mo,Juan Li,Yajun Wang

Brain research 1786:147901 PubMed35367433

2022

Sex differences and the lack of effects of chemogenetic manipulation of pro-opiomelanocortin (POMC) neurons on alcohol consumption in male and female mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jonna M Leyrer-Jackson,Lauren E Hood,M Foster Olive

Frontiers in endocrinology 12:719843 PubMed34497587

2021

3D Atlas of the Pituitary Gland of the Model Fish Medaka ().

Applications

Unspecified application

Species

Unspecified reactive species

Muhammad Rahmad Royan,Khadeeja Siddique,Gergely Csucs,Maja A Puchades,Rasoul Nourizadeh-Lillabadi,Jan G Bjaalie,Christiaan V Henkel,Finn-Arne Weltzien,Romain Fontaine

Cancer immunology, immunotherapy : CII 70:3669-3677 PubMed33977343

2021

Mechanistic insights into immune checkpoint inhibitor-related hypophysitis: a form of paraneoplastic syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Keitaro Kanie,Genzo Iguchi,Hironori Bando,Shin Urai,Hiroki Shichi,Yasunori Fujita,Ryusaku Matsumoto,Kentaro Suda,Masaaki Yamamoto,Hidenori Fukuoka,Wataru Ogawa,Yutaka Takahashi

Frontiers in endocrinology 12:578802 PubMed33679614

2021

Clinical Heterogeneity of Acquired Idiopathic Isolated Adrenocorticotropic Hormone Deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Yasunori Fujita,Hironori Bando,Genzo Iguchi,Keiji Iida,Hitoshi Nishizawa,Keitaro Kanie,Kenichi Yoshida,Ryusaku Matsumoto,Kentaro Suda,Hidenori Fukuoka,Wataru Ogawa,Yutaka Takahashi

Aging 13:1422-1439 PubMed33472171

2020

Functional characterization of DLK1/MEG3 locus on chromosome 14q32.2 reveals the differentiation of pituitary neuroendocrine tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Yiyuan Chen,Hua Gao,Qian Liu,Weiyan Xie,Bin Li,Sen Cheng,Jing Guo,Qiuyue Fang,Haibo Zhu,Zhuang Wang,Jichao Wang,Chuzhong Li,Yazhuo Zhang
View all publications
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