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AB233953

Anti-ACTH antibody [AH26]

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(1 Publication)

Mouse Recombinant Monoclonal POMC antibody. Suitable for IHC-P, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human POMC aa 1-50.

View Alternative Names

Pro-opiomelanocortin, POMC, Corticotropin-lipotropin, Adrenocorticotropic hormone, Adrenocorticotropin, Alpha melanocyte stimulating hormone, Beta LPH, Beta melanocyte stimulating hormone, Beta-endorphin, Corticotropin lipotropin, Corticotropin-like intermediary peptide, Gamma LPH, Lipotropin beta, Lipotropin gamma, Lipotropin, included, MSH, Melanocyte-stimulating hormone, included, Melanotropin alpha, Melanotropin beta, Melanotropin gamma, Melanotropin, included, Met-enkephalin, NPP, Opiomelanocortin prepropeptide, POC, POMC, Pomc-1, Pomc2, Pro ACTH endorphin, Pro opiomelanocortin, Pro-opiomelanocortin-alpha, Proopiomelanocortin preproprotein, Tetracosactide, alpha-MSH, beta-MSH, gamma-MSH

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTH antibody [AH26] (AB233953)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTH antibody [AH26] (AB233953)

Immunohistochemical analysis of paraffin-embedded Human pituitary gland tissue labeling ACTH with ab233953 at 1/5000 (0.233 µg/ml) dilution, followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

Positive staining on human pituitary gland. The section was incubated with ab233953 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879)

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTH antibody [AH26] (AB233953)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTH antibody [AH26] (AB233953)

Immunohistochemical analysis of paraffin-embedded Rat pituitary gland tissue labeling ACTH with ab233953 at 1/10000 (0.116 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat pituitary gland. The section was incubated with ab233953 for 30 mins at room temperature and then combined with specific mouse IgG antibody (ab125913, 1 : 1000) for 8mins.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection)

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTH antibody [AH26] (AB233953)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTH antibody [AH26] (AB233953)

Immunohistochemical analysis of paraffin-embedded Mouse pituitary gland tissue labeling ACTH with ab233953 at 1/10000 (0.116 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse pituitary gland. The section was incubated with ab233953 for 30 mins at room temperature and then combined with specific mouse IgG antibody (ab125913, 1 : 1000) for 8mins.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection)

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Immunohistochemistry (Frozen sections) - Anti-ACTH antibody [AH26] (AB233953)
  • IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-ACTH antibody [AH26] (AB233953)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse pituitary (fresh frozen) tissue labeling ACTH with ab233953 at 1/100 (11.64 µg/ml) dilution followed by ab150113, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green).

Positive staining on mouse pituitary. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluorr® 488) at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

  • Carrier free

    Anti-ACTH antibody [AH26] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

AH26

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

IHC-P, IHC-Fr

applications

Immunogen

Synthetic Peptide within Human POMC aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P01189

Specificity

ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This antibody is specific to Synacthen (aa 1-24 of ACTH); does not react with CLIP (aa 17-39 of ACTH).

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Alternative Products

Primary Antibodies

AB199007

Anti-ACTH antibody [AH26 + 57] - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTH antibody [AH26 + 57] - N-terminal (AB199007)

  • 0
  • 0
Primary Antibodies

AB233954

Anti-ACTH antibody [57] - N-terminal

BOND RX™ Validated|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTH antibody [57] - N-terminal (AB233954)

  • 0
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Primary Antibodies

AB231425

Anti-ACTH antibody [SPM501]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTH antibody [SPM501] (AB231425)

  • 0
  • 0
Primary Antibodies

AB212736

Anti-ACTH antibody [57] - N-terminal- BSA and Azide free

BOND RX™ Validated|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTH antibody [57] - N-terminal- BSA and Azide free (AB212736)

  • 0
  • 0

Complementary Products

Assay Kits

AB267814

Human ACTH ELISA Kit (adrenocorticotropic hormone)

Recombinant|SimpleStep

ELISA - Human ACTH ELISA Kit (adrenocorticotropic hormone) (AB267814)

  • 0
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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB30914

Anti-Neuropeptide Y antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody (AB30914)

  • 5
  • 17
Primary Antibodies

AB177487

Anti-NeuN antibody [EPR12763] - Neuronal Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB177487)

  • 5
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Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Adrenocorticotropic hormone (ACTH) also known as corticotropin is a peptide hormone secreted by the pituitary gland. It is a 39-amino acid polypeptide with a molecular mass of approximately 4540 Da. ACTH is primarily expressed in the anterior pituitary gland and smaller amounts can be found in the central nervous system. Its production and secretion are tightly regulated by corticotropin-releasing hormone (CRH) through a feedback mechanism involving the hypothalamic-pituitary-adrenal (HPA) axis.
Biological function summary

Adrenocorticotropic hormone stimulates the adrenal cortex to produce and release glucocorticoids especially cortisol. ACTH is not part of a larger complex but acts as a singular hormone in its pathway. The hormone also influences the expression of certain enzymes important for steroid biosynthesis. In addition to its role in the adrenal cortex ACTH can mildly interact with melanocortin receptors on adipose tissues contributing to lipolysis and inflammatory processes.

Pathways

Adrenocorticotropic hormone functions in the HPA axis one of the body's key stress response systems. The hormone also involves in the regulation of the corticosteroid biosynthesis pathway. The primary protein it interacts with in these processes is cortisol which in turn regulates many physiological processes including immune response and metabolism. ACTH and cortisol form a feedback loop where elevated cortisol levels inhibit the release of both CRH and ACTH.

Increased or decreased levels of adrenocorticotropic hormone can result in disorders such as Cushing's disease and Addison's disease. Cushing's disease is characterized by overproduction of ACTH often due to pituitary adenomas leading to excess cortisol production. Conversely in Addison’s disease inadequate ACTH or adrenal cortex dysfunction leads to insufficient cortisol production. Both conditions highlight the disrupted feedback relationship between ACTH and cortisol resulting in significant clinical symptoms that require careful management.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Precursor protein of pituitary hormones that are involved in diverse physiological processes, including the regulation of energy balance, stress response, immune function and skin pigmentation.. Corticotropin. Functions as a ligand for the melanocortin receptors MC1R, MC2R, MC3R and MC5R (PubMed : 8396929, PubMed : 8463333, PubMed : 8636348). Activation of MC1R increases melanogenesis in melanocytes found in the skin and hair (PubMed : 9620771). Binding to MC2R stimulates the adrenal glands to secrete cortisol (PubMed : 8636348). Contributes to the regulation of energy homeostasis through activation of MC3R (Probable). Involved in the regulation exocrine gland function through MC5R activation (By similarity).. Melanocyte-stimulating hormone alpha. Serves as a ligand for the melanocortin receptors MC1R, MC3R, MC4R and MC5R (PubMed : 8392067, PubMed : 8396929, PubMed : 8463333). Activation of MC1R promotes melanogenesis in melanocytes of the skin and hair (PubMed : 10403794, PubMed : 9620771). Contributes to the regulation of energy homeostasis through activation of MC3R (Probable). Through MC4R activation, functions as an anorexigenic peptide (PubMed : 8392067). Promotes immunosuppression and involved in the regulation exocrine gland function through MC5R activation (By similarity).. Melanocyte-stimulating hormone beta. Functions as a ligand for the melanocortin receptors MC1R, MC3R, MC4R and MC5R (PubMed : 8396929, PubMed : 8463333). Activation of MC1R increases melanogenesis in melanocytes found in the skin and hair (Probable). Contributes to the regulation of energy homeostasis through activation of MC3R (Probable). Through MC4R activation, functions as an anorexigenic peptide (PubMed : 8392067). Involved in the regulation exocrine gland function through MC5R activation (By similarity).. Melanotropin gamma. Functions as a ligand for the melanocortin receptors MC1R, MC3R, MC4R and MC5R (PubMed : 8396929, PubMed : 8463333). Activation of MC1R increases melanogenesis in melanocytes found in the skin and hair (Probable). Contributes to the regulation of energy homeostasis through activation of MC3R (Probable). Binds to MC4R with low potency (PubMed : 8392067). Involved in the regulation exocrine gland function through MC5R activation (By similarity).. Beta-endorphin. Endogenous orexigenic opiate.. Met-enkephalin. Endogenous opiate.
See full target information POMC

Additional targets

ACTH
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 285:12670-83 PubMed20139084

2010

Down-regulation of MyoD by calpain 3 promotes generation of reserve cells in C2C12 myoblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Pascal Stuelsatz,Frédéric Pouzoulet,Yann Lamarre,Elise Dargelos,Sylvie Poussard,Serge Leibovitch,Patrick Cottin,Philippe Veschambre
View all publications
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Product promise

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