AB259687

Anti-ACTH antibody [EPR20361-248] - BSA and Azide free (Capture)

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal POMC antibody. Carrier free. Suitable for sELISA and reacts with Mouse, Rat, Human samples.

View Alternative Names

Pro-opiomelanocortin, POMC, Corticotropin-lipotropin

1 Images

Sandwich ELISA - Anti-ACTH antibody [EPR20361-248] - BSA and Azide free (Capture) (AB259687)

sELISA

Supplier Data

Sandwich ELISA - Anti-ACTH antibody [EPR20361-248] - BSA and Azide free (Capture) (AB259687)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab267814).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20361-248

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "" }, "Mouse": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "" }, "Rat": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "" } } }

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Product details

ab259687 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human/Mouse/Rat ACTH. The recommended pair for sandwich ELISA is:
Capture: ab259687, Human/Mouse/Rat ACTH Capture Antibody (unconjugated)
Detector: ab259688, Human/Mouse/Rat ACTH Detector Antibody (unconjugated)
The reference range value is 2.5 - 160 pg/ml for human and 7.8125 - 500 ng/ml for mouse/rat.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Adrenocorticotropic hormone (ACTH) also known as corticotropin is a peptide hormone secreted by the pituitary gland. It is a 39-amino acid polypeptide with a molecular mass of approximately 4540 Da. ACTH is primarily expressed in the anterior pituitary gland and smaller amounts can be found in the central nervous system. Its production and secretion are tightly regulated by corticotropin-releasing hormone (CRH) through a feedback mechanism involving the hypothalamic-pituitary-adrenal (HPA) axis.

Biological function summary

Adrenocorticotropic hormone stimulates the adrenal cortex to produce and release glucocorticoids especially cortisol. ACTH is not part of a larger complex but acts as a singular hormone in its pathway. The hormone also influences the expression of certain enzymes important for steroid biosynthesis. In addition to its role in the adrenal cortex ACTH can mildly interact with melanocortin receptors on adipose tissues contributing to lipolysis and inflammatory processes.

Pathways

Adrenocorticotropic hormone functions in the HPA axis one of the body's key stress response systems. The hormone also involves in the regulation of the corticosteroid biosynthesis pathway. The primary protein it interacts with in these processes is cortisol which in turn regulates many physiological processes including immune response and metabolism. ACTH and cortisol form a feedback loop where elevated cortisol levels inhibit the release of both CRH and ACTH.

Increased or decreased levels of adrenocorticotropic hormone can result in disorders such as Cushing's disease and Addison's disease. Cushing's disease is characterized by overproduction of ACTH often due to pituitary adenomas leading to excess cortisol production. Conversely in Addison’s disease inadequate ACTH or adrenal cortex dysfunction leads to insufficient cortisol production. Both conditions highlight the disrupted feedback relationship between ACTH and cortisol resulting in significant clinical symptoms that require careful management.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Corticotropin. Stimulates the adrenal glands to release cortisol.. Melanocyte-stimulating hormone alpha. Anorexigenic peptide. Increases the pigmentation of skin by increasing melanin production in melanocytes.. Melanocyte-stimulating hormone beta. Increases the pigmentation of skin by increasing melanin production in melanocytes.. Beta-endorphin. Endogenous orexigenic opiate.. Met-enkephalin. Endogenous opiate.

See full target information POMC

Additional targets

ACTH

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ACTH antibody [EPR20361-248] - BSA and Azide free (Capture)

Sandwich ELISA - Anti-ACTH antibody [EPR20361-248] - BSA and Azide free (Capture) (AB259687)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise