Mouse Monoclonal alpha skeletal muscle Actin antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Native Full Length Protein corresponding to Cow ACTA1.
Preservative: 0.03% Sodium azide
Constituents: 49.98% Glycerol (glycerin, glycerine), 49.98% PBS
WB | ICC/IF | |
---|---|---|
Human | Tested | Tested |
Mouse | Tested | Expected |
Rat | Tested | Expected |
Orangutan | Predicted | Predicted |
Rabbit | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rabbit, Orangutan | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rabbit, Orangutan | Dilution info - | Notes - |
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Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
ACTA, ACTA1, Alpha-actin-1
Mouse Monoclonal alpha skeletal muscle Actin antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Native Full Length Protein corresponding to Cow ACTA1.
Preservative: 0.03% Sodium azide
Constituents: 49.98% Glycerol (glycerin, glycerine), 49.98% PBS
ab190301 binds to all six isotypes of mammalian actin.
Actin also known as globular (G-actin) or filamentous (F-actin) actin plays a central role in cell structure and movement. This protein has a molecular weight of approximately 42 kDa and resides abundantly in eukaryotic cells especially in muscle and cytoskeletal structures. It comes in several isoforms with varying expression profiles across tissues. Actin can undergo polymerization from its G-actin monomers into F-actin filaments a process that is reversible through depolymerization allowing for dynamic cellular functions.
Actin contributes to various cellular processes by forming the cytoskeleton which provides mechanical support and determines cell shape. Actin also facilitates cell motility division and intracellular transport through rapid polymerization and depolymerization cycles. Within cells actin associates with other proteins to form complexes such as the Arp2/3 complex which assists actin in the branching of filaments critical for pushing forward the cell's leading edge during movement. Techniques like actin immunofluorescence help visualize actin filaments within cells revealing its extensive network.
Actin plays an integral role in pathways like cell movement and signal transduction. The Rho family of GTPases regulates actin cytoskeleton rearrangements influencing cell shape and migration. Actin function interactions involve proteins like myosin forming actomyosin complexes essential for muscle contraction and other cell motility activities. Actin polymerization and depolymerization cycles are key to the dynamic regulation within these pathways ensuring adequate cellular responses to environmental signals.
Mutations or misregulation of actin and its associated pathways link to conditions such as cardiomyopathies and cancer metastasis. In familial cardiomyopathy actin mutations disrupt normal cardiac muscle contraction. In cancer altered actin polymerization and depolymerization enable invasive cell migration facilitating metastasis. Proteins like myosin and tropomyosin associate with actin in these pathologies illustrating the impact of actin dynamics on disease progression and highlighting actin as a therapeutic target. Anti-actin antibodies can be used in research and diagnostics to better understand these disorders.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Actin Western blot staining using mouse Anti-Actin antibody
The same blot was simultaneously probed with chicken pAb to UCHL-1, a marker of neuronal lineage cells (green).
All lanes: Western blot - Anti-Actin antibody [5J11] - Loading Control (ab190301) at 1/1000 dilution
Lane 1: Protein standard
Lane 2: Rat brain lysate
Lane 3: Mouse brain lysate
Lane 4: NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 5: HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 6: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 7: SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate
Predicted band size: 42 kDa
Immunofluorescent analysis of HeLa cells labeling Actin with ab190301 at 1/1000 dilution (green) and chicken polyclonal antibody to Vimentin (red). The blue stain reveals DNA in the nuclei of these cells.
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