AB190301

Anti-Actin antibody [5J11] - Loading Control

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(4 Publications)

Mouse Monoclonal alpha skeletal muscle Actin antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Native Full Length Protein corresponding to Cow ACTA1.

View Alternative Names

ACTA, ACTA1, Alpha-actin-1

2 Images

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [5J11] - Loading Control (AB190301)

Immunofluorescent analysis of HeLa cells labeling Actin with ab190301 at 1/1000 dilution (green) and chicken polyclonal antibody to Vimentin (red). The blue stain reveals DNA in the nuclei of these cells.

Supplier Data

Western blot - Anti-Actin antibody [5J11] - Loading Control (AB190301)

The same blot was simultaneously probed with chicken pAb to UCHL-1, a marker of neuronal lineage cells (green).

All lanes:

Western blot - Anti-Actin antibody [5J11] - Loading Control (ab190301) at 1/1000 dilution

Lane 1:

Protein standard

Lane 2:

Rat brain lysate

Lane 3:

Mouse brain lysate

Lane 4:

NIH/3T3 (mouse embryo fibroblast cell line) cell lysate

Lane 5:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Lane 6:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Lane 7:

SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate

Predicted band size: 42 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5J11

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

Native Full Length Protein corresponding to Cow ACTA1.

P68138

Specificity

ab190301 binds to all six isotypes of mammalian actin.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.03% Sodium azide Constituents: PBS, 49.98% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Actin also known as globular (G-actin) or filamentous (F-actin) actin plays a central role in cell structure and movement. This protein has a molecular weight of approximately 42 kDa and resides abundantly in eukaryotic cells especially in muscle and cytoskeletal structures. It comes in several isoforms with varying expression profiles across tissues. Actin can undergo polymerization from its G-actin monomers into F-actin filaments a process that is reversible through depolymerization allowing for dynamic cellular functions.

Biological function summary

Actin contributes to various cellular processes by forming the cytoskeleton which provides mechanical support and determines cell shape. Actin also facilitates cell motility division and intracellular transport through rapid polymerization and depolymerization cycles. Within cells actin associates with other proteins to form complexes such as the Arp2/3 complex which assists actin in the branching of filaments critical for pushing forward the cell's leading edge during movement. Techniques like actin immunofluorescence help visualize actin filaments within cells revealing its extensive network.

Pathways

Actin plays an integral role in pathways like cell movement and signal transduction. The Rho family of GTPases regulates actin cytoskeleton rearrangements influencing cell shape and migration. Actin function interactions involve proteins like myosin forming actomyosin complexes essential for muscle contraction and other cell motility activities. Actin polymerization and depolymerization cycles are key to the dynamic regulation within these pathways ensuring adequate cellular responses to environmental signals.

Mutations or misregulation of actin and its associated pathways link to conditions such as cardiomyopathies and cancer metastasis. In familial cardiomyopathy actin mutations disrupt normal cardiac muscle contraction. In cancer altered actin polymerization and depolymerization enable invasive cell migration facilitating metastasis. Proteins like myosin and tropomyosin associate with actin in these pathologies illustrating the impact of actin dynamics on disease progression and highlighting actin as a therapeutic target. Anti-actin antibodies can be used in research and diagnostics to better understand these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

See full target information ACTA1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:10250 PubMed40133513

2025

Connexin 43 is downregulated in advanced Parkinson's disease in multiple brain regions which correlates with symptoms.

Applications

Unspecified application

Species

Unspecified reactive species

Nataly Hastings,Saifur Rahman,Przemyslaw Aleksander Stempor,Matthew T Wayland,Wei-Li Kuan,Mark R N Kotter

Biomolecules 12: PubMed35625661

2022

Impairment of the Retinal Endothelial Cell Barrier Induced by Long-Term Treatment with VEGF-A No Longer Depends on the Growth Factor's Presence.

Applications

Unspecified application

Species

Unspecified reactive species

Heidrun L Deissler,Matus Rehak,Armin Wolf

Heliyon 7:e06037 PubMed33521368

2021

Type of culture medium determines properties of cultivated retinal endothelial cells: induction of substantial phenotypic conversion by standard DMEM.

Applications

Unspecified application

Species

Unspecified reactive species

Catharina Busch,Matus Rehak,Margrit Hollborn,Peter Wiedemann,Gerhard K Lang,Gabriele E Lang,Armin Wolf,Heidrun L Deissler

Journal of diabetes research 2020:2450781 PubMed32566677

2020

Sitagliptin and the Blood-Retina Barrier: Effects on Retinal Endothelial Cells Manifested Only after Prolonged Exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Anja Jäckle,Focke Ziemssen,Eva-Maria Kuhn,Jürgen Kampmeier,Gerhard K Lang,Gabriele E Lang,Helmut Deissler,Heidrun L Deissler

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Actin antibody [5J11] - Loading Control

Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [5J11] - Loading Control (AB190301)

Western blot - Anti-Actin antibody [5J11] - Loading Control (AB190301)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise