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Mouse Monoclonal alpha skeletal muscle Actin antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Native Full Length Protein corresponding to Cow ACTA1.

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Images

Western blot - Anti-Actin antibody [5J11] - Loading Control (AB190301), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [5J11] (AB190301), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

Preservative: 0.03% Sodium azide
Constituents: 49.98% Glycerol (glycerin, glycerine), 49.98% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Native Full Length Protein corresponding to Cow ACTA1. Database link P68138

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested
Mouse
Tested
Expected
Rat
Tested
Expected
Orangutan
Predicted
Predicted
Rabbit
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Predicted
Predicted

Species
Rabbit, Orangutan
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500.00000 - 1/1000.00000
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rabbit, Orangutan
Dilution info
-
Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Alternative names

Recommended products

Mouse Monoclonal alpha skeletal muscle Actin antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Native Full Length Protein corresponding to Cow ACTA1.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Native Full Length Protein corresponding to Cow ACTA1. Database link P68138
Clone number
5J11
Purification technique
Affinity purification Protein G
Specificity

ab190301 binds to all six isotypes of mammalian actin.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Actin also known as globular (G-actin) or filamentous (F-actin) actin plays a central role in cell structure and movement. This protein has a molecular weight of approximately 42 kDa and resides abundantly in eukaryotic cells especially in muscle and cytoskeletal structures. It comes in several isoforms with varying expression profiles across tissues. Actin can undergo polymerization from its G-actin monomers into F-actin filaments a process that is reversible through depolymerization allowing for dynamic cellular functions.

Biological function summary

Actin contributes to various cellular processes by forming the cytoskeleton which provides mechanical support and determines cell shape. Actin also facilitates cell motility division and intracellular transport through rapid polymerization and depolymerization cycles. Within cells actin associates with other proteins to form complexes such as the Arp2/3 complex which assists actin in the branching of filaments critical for pushing forward the cell's leading edge during movement. Techniques like actin immunofluorescence help visualize actin filaments within cells revealing its extensive network.

Pathways

Actin plays an integral role in pathways like cell movement and signal transduction. The Rho family of GTPases regulates actin cytoskeleton rearrangements influencing cell shape and migration. Actin function interactions involve proteins like myosin forming actomyosin complexes essential for muscle contraction and other cell motility activities. Actin polymerization and depolymerization cycles are key to the dynamic regulation within these pathways ensuring adequate cellular responses to environmental signals.

Associated diseases and disorders

Mutations or misregulation of actin and its associated pathways link to conditions such as cardiomyopathies and cancer metastasis. In familial cardiomyopathy actin mutations disrupt normal cardiac muscle contraction. In cancer altered actin polymerization and depolymerization enable invasive cell migration facilitating metastasis. Proteins like myosin and tropomyosin associate with actin in these pathologies illustrating the impact of actin dynamics on disease progression and highlighting actin as a therapeutic target. Anti-actin antibodies can be used in research and diagnostics to better understand these disorders.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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2 product images

  • Western blot - Anti-Actin antibody [5J11] - Loading Control (ab190301), expandable thumbnail

    Western blot - Anti-Actin antibody [5J11] - Loading Control (ab190301)

    Actin Western blot staining using mouse Anti-Actin antibody

    The same blot was simultaneously probed with chicken pAb to UCHL-1, a marker of neuronal lineage cells (green).

    All lanes: Western blot - Anti-Actin antibody [5J11] - Loading Control (ab190301) at 1/1000 dilution

    Lane 1: Protein standard

    Lane 2: Rat brain lysate

    Lane 3: Mouse brain lysate

    Lane 4: NIH/3T3 (mouse embryo fibroblast cell line) cell lysate

    Lane 5: HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

    Lane 6: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

    Lane 7: SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate

    Predicted band size: 42 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [5J11] (ab190301), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [5J11] (ab190301)

    Immunofluorescent analysis of HeLa cells labeling Actin with ab190301 at 1/1000 dilution (green) and chicken polyclonal antibody to Vimentin (red). The blue stain reveals DNA in the nuclei of these cells.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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