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Rabbit Polyclonal alpha skeletal muscle Actin antibody. Suitable for IHC, WB, IHC-P, IHC - Wmt and reacts with Zebrafish samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ACTA1 aa 1-200.

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Images

Immunohistochemistry - Anti-Actin antibody (AB209857), expandable thumbnail
  • Western blot - Anti-Actin antibody (AB209857), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody (AB209857), expandable thumbnail
  • IHC - Wholemount - Anti-Actin antibody (AB209857), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human ACTA1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P68133

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHCWBIHC-PIHC - Wmt
Zebrafish
Tested
Tested
Tested
Tested

Tested
Tested

Species

Zebrafish

Dilution info

1/100 - 1/1000

Notes

-

Tested
Tested

Species

Zebrafish

Dilution info

1/500 - 1/3000

Notes

-

Tested
Tested

Species

Zebrafish

Dilution info

1/100 - 1/1000

Notes

Suggested antigen retrieval using 1mM EDTA-NaOH (pH8.0) 95C,1Hr

Tested
Tested

Species

Zebrafish

Dilution info

1/50 - 1/500

Notes

-

Associated Products

Select an associated product type

9 products for Alternative Product

Target data

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Alternative names

Recommended products

Rabbit Polyclonal alpha skeletal muscle Actin antibody. Suitable for IHC, WB, IHC-P, IHC - Wmt and reacts with Zebrafish samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ACTA1 aa 1-200.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human ACTA1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P68133
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Actin also known as globular (G-actin) or filamentous (F-actin) actin plays a central role in cell structure and movement. This protein has a molecular weight of approximately 42 kDa and resides abundantly in eukaryotic cells especially in muscle and cytoskeletal structures. It comes in several isoforms with varying expression profiles across tissues. Actin can undergo polymerization from its G-actin monomers into F-actin filaments a process that is reversible through depolymerization allowing for dynamic cellular functions.

Biological function summary

Actin contributes to various cellular processes by forming the cytoskeleton which provides mechanical support and determines cell shape. Actin also facilitates cell motility division and intracellular transport through rapid polymerization and depolymerization cycles. Within cells actin associates with other proteins to form complexes such as the Arp2/3 complex which assists actin in the branching of filaments critical for pushing forward the cell's leading edge during movement. Techniques like actin immunofluorescence help visualize actin filaments within cells revealing its extensive network.

Pathways

Actin plays an integral role in pathways like cell movement and signal transduction. The Rho family of GTPases regulates actin cytoskeleton rearrangements influencing cell shape and migration. Actin function interactions involve proteins like myosin forming actomyosin complexes essential for muscle contraction and other cell motility activities. Actin polymerization and depolymerization cycles are key to the dynamic regulation within these pathways ensuring adequate cellular responses to environmental signals.

Associated diseases and disorders

Mutations or misregulation of actin and its associated pathways link to conditions such as cardiomyopathies and cancer metastasis. In familial cardiomyopathy actin mutations disrupt normal cardiac muscle contraction. In cancer altered actin polymerization and depolymerization enable invasive cell migration facilitating metastasis. Proteins like myosin and tropomyosin associate with actin in these pathologies illustrating the impact of actin dynamics on disease progression and highlighting actin as a therapeutic target. Anti-actin antibodies can be used in research and diagnostics to better understand these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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4 product images

  • Immunohistochemistry - Anti-Actin antibody (ab209857), expandable thumbnail

    Immunohistochemistry - Anti-Actin antibody (ab209857)

    Immunohistochemical analysis of agarose-embedded Zebrafish embryo tail section labeling Actin with ab209857 at 1/200 dilution.

  • Western blot - Anti-Actin antibody (ab209857), expandable thumbnail

    Western blot - Anti-Actin antibody (ab209857)

    10% SDS-PAGE

    All lanes: Western blot - Anti-Actin antibody (ab209857) at 1/1000 dilution

    All lanes: whole zebrafish extract at 30 µg

    Predicted band size: 42 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody (ab209857), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody (ab209857)

    Immunohistochemical analysis of paraffin-embedded zebrafish tissue labeling Actin with ab209857 at 1/300 dilution.

  • IHC - Wholemount - Anti-Actin antibody (ab209857), expandable thumbnail

    IHC - Wholemount - Anti-Actin antibody (ab209857)

    Whole mount immunohistochemical analysis of of 1 day-post-fertilization zebrafish embryo labeling Actin with ab209857 at 1/50 dilution.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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