Rabbit Polyclonal alpha skeletal muscle Actin antibody. Suitable for IHC, WB, IHC-P, IHC - Wmt and reacts with Zebrafish samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ACTA1 aa 1-200.
IgG
Rabbit
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
IHC | WB | IHC-P | IHC - Wmt | |
---|---|---|---|---|
Zebrafish | Tested | Tested | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Zebrafish | Dilution info 1/100 - 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Zebrafish | Dilution info 1/500 - 1/3000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Zebrafish | Dilution info 1/100 - 1/1000 | Notes Suggested antigen retrieval using 1mM EDTA-NaOH (pH8.0) 95C,1Hr |
Species | Dilution info | Notes |
---|---|---|
Species Zebrafish | Dilution info 1/50 - 1/500 | Notes - |
Select an associated product type
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
ACTA, ACTA1, ACTA, Alpha-actin-1
Rabbit Polyclonal alpha skeletal muscle Actin antibody. Suitable for IHC, WB, IHC-P, IHC - Wmt and reacts with Zebrafish samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ACTA1 aa 1-200.
IgG
Rabbit
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
Actin also known as globular (G-actin) or filamentous (F-actin) actin plays a central role in cell structure and movement. This protein has a molecular weight of approximately 42 kDa and resides abundantly in eukaryotic cells especially in muscle and cytoskeletal structures. It comes in several isoforms with varying expression profiles across tissues. Actin can undergo polymerization from its G-actin monomers into F-actin filaments a process that is reversible through depolymerization allowing for dynamic cellular functions.
Actin contributes to various cellular processes by forming the cytoskeleton which provides mechanical support and determines cell shape. Actin also facilitates cell motility division and intracellular transport through rapid polymerization and depolymerization cycles. Within cells actin associates with other proteins to form complexes such as the Arp2/3 complex which assists actin in the branching of filaments critical for pushing forward the cell's leading edge during movement. Techniques like actin immunofluorescence help visualize actin filaments within cells revealing its extensive network.
Actin plays an integral role in pathways like cell movement and signal transduction. The Rho family of GTPases regulates actin cytoskeleton rearrangements influencing cell shape and migration. Actin function interactions involve proteins like myosin forming actomyosin complexes essential for muscle contraction and other cell motility activities. Actin polymerization and depolymerization cycles are key to the dynamic regulation within these pathways ensuring adequate cellular responses to environmental signals.
Mutations or misregulation of actin and its associated pathways link to conditions such as cardiomyopathies and cancer metastasis. In familial cardiomyopathy actin mutations disrupt normal cardiac muscle contraction. In cancer altered actin polymerization and depolymerization enable invasive cell migration facilitating metastasis. Proteins like myosin and tropomyosin associate with actin in these pathologies illustrating the impact of actin dynamics on disease progression and highlighting actin as a therapeutic target. Anti-actin antibodies can be used in research and diagnostics to better understand these disorders.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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Immunohistochemical analysis of agarose-embedded Zebrafish embryo tail section labeling Actin with ab209857 at 1/200 dilution.
10% SDS-PAGE
All lanes: Western blot - Anti-Actin antibody (ab209857) at 1/1000 dilution
All lanes: whole zebrafish extract at 30 µg
Predicted band size: 42 kDa
Immunohistochemical analysis of paraffin-embedded zebrafish tissue labeling Actin with ab209857 at 1/300 dilution.
Whole mount immunohistochemical analysis of of 1 day-post-fertilization zebrafish embryo labeling Actin with ab209857 at 1/50 dilution.
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