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Mouse Monoclonal alpha skeletal muscle Actin antibody. Suitable for Dot, WB, IHC-FoFr, IHC-P, ICC/IF, IHC-Fr, ELISA and reacts with Guinea pig, Pig, Hamster, Snail, Carp, Xenopus laevis, Chicken, Human, Dog, Cow, Goat, Sheep, Rabbit, Mouse, Rat samples. Cited in 52 publications. Immunogen corresponding to Synthetic Peptide within Human ACTA1 aa 350 to C-terminus.
Alternative names=Alpha-actin-1, ACTA1, ACTA
- Isotype
IgG2a
- Host species
Mouse
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
Synthetic Peptide within Human ACTA1 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link: P68133
Reactivity data
Select an application| Dot | WB | IHC-FoFr | IHC-P | ICC/IF | IHC-Fr | ELISA | |
|---|---|---|---|---|---|---|---|
| Human | Expected | Expected | Expected | Expected | Expected | Tested | Expected |
| Mouse | Expected | Expected | Expected | Expected | Expected | Predicted | Expected |
| Rat | Expected | Expected | Expected | Expected | Expected | Predicted | Expected |
| Carp | Expected | Expected | Expected | Expected | Expected | Predicted | Expected |
| Chicken | Expected | Expected | Expected | Expected | Expected | Predicted | Expected |
| Cow | Expected | Expected | Expected | Expected | Expected | Predicted | Expected |
| Dog | Expected | Expected | Expected | Expected | Expected | Predicted | Expected |
| Goat | Expected | Expected | Expected | Expected | Expected | Predicted | Expected |
| Guinea pig | Expected | Expected | Expected | Expected | Expected | Predicted | Expected |
| Hamster | Expected | Expected | Expected | Expected | Expected | Predicted | Expected |
| Pig | Expected | Expected | Expected | Expected | Expected | Predicted | Expected |
| Rabbit | Expected | Expected | Expected | Expected | Expected | Predicted | Expected |
| Sheep | Expected | Expected | Expected | Expected | Expected | Predicted | Expected |
| Snail | Expected | Expected | Expected | Expected | Expected | Predicted | Expected |
| Xenopus laevis | Expected | Expected | Expected | Expected | Expected | Predicted | Expected |
Images
Dot
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Guinea pig, Pig, Hamster, Snail, Carp, Xenopus laevis, Chicken, Human, Dog, Cow, Goat, Sheep, Rabbit, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes 1/500 using cultured Human or chicken fibroblast extract. Predicted molecular weight: 42 kDa. |
Species Rat | Dilution info - | Notes 1/500 using cultured Human or chicken fibroblast extract. Predicted molecular weight: 42 kDa. |
Species Guinea pig | Dilution info - | Notes 1/500 using cultured Human or chicken fibroblast extract. Predicted molecular weight: 42 kDa. |
Species Pig | Dilution info - | Notes 1/500 using cultured Human or chicken fibroblast extract. Predicted molecular weight: 42 kDa. |
Species Hamster | Dilution info - | Notes 1/500 using cultured Human or chicken fibroblast extract. Predicted molecular weight: 42 kDa. |
Species Snail | Dilution info - | Notes 1/500 using cultured Human or chicken fibroblast extract. Predicted molecular weight: 42 kDa. |
Species Carp | Dilution info - | Notes 1/500 using cultured Human or chicken fibroblast extract. Predicted molecular weight: 42 kDa. |
Species Xenopus laevis | Dilution info - | Notes 1/500 using cultured Human or chicken fibroblast extract. Predicted molecular weight: 42 kDa. |
Species Chicken | Dilution info - | Notes 1/500 using cultured Human or chicken fibroblast extract. Predicted molecular weight: 42 kDa. |
Species Human | Dilution info - | Notes 1/500 using cultured Human or chicken fibroblast extract. Predicted molecular weight: 42 kDa. |
Species Dog | Dilution info - | Notes 1/500 using cultured Human or chicken fibroblast extract. Predicted molecular weight: 42 kDa. |
Species Cow | Dilution info - | Notes 1/500 using cultured Human or chicken fibroblast extract. Predicted molecular weight: 42 kDa. |
Species Goat | Dilution info - | Notes 1/500 using cultured Human or chicken fibroblast extract. Predicted molecular weight: 42 kDa. |
Species Sheep | Dilution info - | Notes 1/500 using cultured Human or chicken fibroblast extract. Predicted molecular weight: 42 kDa. |
Species Rabbit | Dilution info - | Notes 1/500 using cultured Human or chicken fibroblast extract. Predicted molecular weight: 42 kDa. |
IHC-FoFr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Guinea pig, Pig, Hamster, Snail, Carp, Xenopus laevis, Chicken, Human, Dog, Cow, Goat, Sheep, Rabbit | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Guinea pig, Pig, Hamster, Snail, Carp, Xenopus laevis, Chicken, Human, Dog, Cow, Goat, Sheep, Rabbit | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes 1/200 determined by indirect immunofluorescent staining of cultured Human or chicken fibroblasts. |
Species Rat | Dilution info - | Notes 1/200 determined by indirect immunofluorescent staining of cultured Human or chicken fibroblasts. |
Species Guinea pig | Dilution info - | Notes 1/200 determined by indirect immunofluorescent staining of cultured Human or chicken fibroblasts. |
Species Pig | Dilution info - | Notes 1/200 determined by indirect immunofluorescent staining of cultured Human or chicken fibroblasts. |
Species Hamster | Dilution info - | Notes 1/200 determined by indirect immunofluorescent staining of cultured Human or chicken fibroblasts. |
Species Snail | Dilution info - | Notes 1/200 determined by indirect immunofluorescent staining of cultured Human or chicken fibroblasts. |
Species Carp | Dilution info - | Notes 1/200 determined by indirect immunofluorescent staining of cultured Human or chicken fibroblasts. |
Species Xenopus laevis | Dilution info - | Notes 1/200 determined by indirect immunofluorescent staining of cultured Human or chicken fibroblasts. |
Species Chicken | Dilution info - | Notes 1/200 determined by indirect immunofluorescent staining of cultured Human or chicken fibroblasts. |
Species Human | Dilution info - | Notes 1/200 determined by indirect immunofluorescent staining of cultured Human or chicken fibroblasts. |
Species Dog | Dilution info - | Notes 1/200 determined by indirect immunofluorescent staining of cultured Human or chicken fibroblasts. |
Species Cow | Dilution info - | Notes 1/200 determined by indirect immunofluorescent staining of cultured Human or chicken fibroblasts. |
Species Goat | Dilution info - | Notes 1/200 determined by indirect immunofluorescent staining of cultured Human or chicken fibroblasts. |
Species Sheep | Dilution info - | Notes 1/200 determined by indirect immunofluorescent staining of cultured Human or chicken fibroblasts. |
Species Rabbit | Dilution info - | Notes 1/200 determined by indirect immunofluorescent staining of cultured Human or chicken fibroblasts. |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Pig, Xenopus laevis, Rat, Snail, Rabbit, Dog, Mouse, Chicken, Hamster, Guinea pig, Cow, Carp, Goat | Dilution info - | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Guinea pig, Pig, Hamster, Snail, Carp, Xenopus laevis, Chicken, Human, Dog, Cow, Goat, Sheep, Rabbit | Dilution info Use at an assay dependent concentration. | Notes - |
Recommended products
Mouse Monoclonal alpha skeletal muscle Actin antibody. Suitable for Dot, WB, IHC-FoFr, IHC-P, ICC/IF, IHC-Fr, ELISA and reacts with Guinea pig, Pig, Hamster, Snail, Carp, Xenopus laevis, Chicken, Human, Dog, Cow, Goat, Sheep, Rabbit, Mouse, Rat samples. Cited in 52 publications. Immunogen corresponding to Synthetic Peptide within Human ACTA1 aa 350 to C-terminus.
Alternative names=Alpha-actin-1, ACTA1, ACTA
Key facts
- Isotype
IgG2a
- Host species
Mouse
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
Synthetic Peptide within Human ACTA1 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link: P68133
- Clone number
AC-40
- Purity
Tissue culture supernatant
- Epitope
Monoclonal anti-Actin recognizes an epitope located on the C-terminal end of actin. This epitope is conserved in all actin isoforms.
- Concentration
- Purification notes
Purified from TCS.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
This product was changed from ascites to tissue culture supernatant on 21 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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