Mouse Monoclonal alpha skeletal muscle Actin antibody. Suitable for Dot, WB, IHC-FoFr, IHC-P, ICC/IF, IHC-Fr, ELISA and reacts with Guinea pig, Pig, Hamster, Snail, Carp, Xenopus laevis, Chicken, Human, Dog, Cow, Goat, Sheep, Rabbit, Mouse, Rat samples. Cited in 52 publications. Immunogen corresponding to Synthetic Peptide within Human ACTA1 aa 350 to C-terminus.
Images
![Immunohistochemistry (Frozen sections) - Anti-Actin antibody [AC-40] - Loading Control (AB11003), expandable thumbnail](https://content.abcam.com/products/images/actin-antibody-ac-40-loading-control-ab11003--immunohistochemistry-frozen-sections-img107832.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Actin antibody [AC-40] - Loading Control (AB11003)")
Publications
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- Genes 14:2023Amplicon Contains Cyclic AMP Response Element-Driven Gene in Taxane-Resistant MCF-7 Breast Cancer Sublines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPetr Daniel,Kamila Balušíková,Radka Václavíková,Karolína Šeborová,Šárka Ransdorfová,Marie Valeriánová,Longfei Wei,Michael Jelínek,Tereza Tlapáková,Thomas Fleischer,Vessela N Kristensen,Pavel Souček,Iwao Ojima,Jan KovářPubMed 36833223
- The Journal of cell biology 222:2022Human septins organize as octamer-based filaments and mediate actin-membrane anchoring in cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarla Silva Martins,Cyntia Taveneau,Gerard Castro-Linares,Mikhail Baibakov,Nicolas Buzhinsky,Mar Eroles,Violeta Milanović,Shizue Omi,Jean-Denis Pedelacq,Francois Iv,Léa Bouillard,Alexander Llewellyn,Maxime Gomes,Mayssa Belhabib,Mira Kuzmić,Pascal Verdier-Pinard,Stacey Lee,Ali Badache,Sanjay Kumar,Cristel Chandre,Sophie Brasselet,Felix Rico,Olivier Rossier,Gijsje H Koenderink,Jerome Wenger,Stéphanie Cabantous,Manos MavrakisPubMed 36562751
- Nature communications 12:66682021MDA5 disease variant M854K prevents ATP-dependent structural discrimination of viral and cellular RNA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQin Yu,Alba Herrero Del Valle,Rahul Singh,Yorgo ModisPubMed 34795277
- Cancers 13:2021Atypical Teratoid Rhabdoid Tumours Are Susceptible to Panobinostat-Mediated Differentiation Therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWai C Chong,W Samantha N Jayasekara,Vijesh G Vaghjiani,Sarah Parackal,Claire Sun,Dean Popovski,Elizabeth M Algar,Ron Firestein,Paul J Wood,Sara Khan,Annie Huang,David M Ashley,Peter Downie,Jason E CainPubMed 34680294
- Experimental and therapeutic medicine 22:13112021Blocking tumor necrosis factor-α delays progression of chronic obstructive pulmonary disease in rats through inhibiting MAPK signaling pathway and activating SOCS3/TRAF1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiong Feng,Yan-Zi Yu,Qing-Hua MengPubMed 34630665
- Central-European journal of immunology 46:140-1512021Knockdown of circular RNA hsa_circ_0003204 inhibits oxidative stress and apoptosis through the miR-330-5p/Nod2 axis to ameliorate endothelial cell injury induced by low-density lipoprotein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBin Zhang,Yufan Zhang,Rui Li,Yan Li,Wei YanPubMed 34764783
- Molecular medicine reports 22:5339-53472020Endothelial cell‑derived CCL15 mediates the transmigration of fibrocytes through the CCL15‑CCR1 axis in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNan Pang et. al.PubMed 33174007
- Cell reports 33:1082352020Temporal Proteomic Analysis of Herpes Simplex Virus 1 Infection Reveals Cell-Surface Remodeling via pUL56-Mediated GOPC Degradation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTimothy K Soh,Colin T R Davies,Julia Muenzner,Leah M Hunter,Henry G Barrow,Viv Connor,Clément R Bouton,Cameron Smith,Edward Emmott,Robin Antrobus,Stephen C Graham,Michael P Weekes,Colin M CrumpPubMed 33027661
- Journal of diabetes research 2020:24507812020Sitagliptin and the Blood-Retina Barrier: Effects on Retinal Endothelial Cells Manifested Only after Prolonged Exposure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnja Jäckle et. al.PubMed 32566677
- Scientific reports 9:180262019Upregulation of vitamin D-binding protein is associated with changes in insulin production in pancreatic beta-cells exposed to p,p'-DDT and p,p'-DDE.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNela Pavlíková et. al.PubMed 31792309
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human ACTA1 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P68133
Reactivity data
Select an application| Dot | WB | IHC-FoFr | IHC-P | ICC/IF | IHC-Fr | ELISA | |
|---|---|---|---|---|---|---|---|
| Human | Expected | Expected | Expected | Expected | Expected | Tested | Expected |
| Mouse | Expected | Expected | Expected | Expected | Expected | Predicted | Expected |
| Rat | Expected | Expected | Expected | Expected | Expected | Predicted | Expected |
| Carp | Expected | Expected | Expected | Expected | Expected | Predicted | Expected |
| Chicken | Expected | Expected | Expected | Expected | Expected | Predicted | Expected |
| Cow | Expected | Expected | Expected | Expected | Expected | Predicted | Expected |
| Dog | Expected | Expected | Expected | Expected | Expected | Predicted | Expected |
| Goat | Expected | Expected | Expected | Expected | Expected | Predicted | Expected |
| Guinea pig | Expected | Expected | Expected | Expected | Expected | Predicted | Expected |
| Hamster | Expected | Expected | Expected | Expected | Expected | Predicted | Expected |
| Pig | Expected | Expected | Expected | Expected | Expected | Predicted | Expected |
| Rabbit | Expected | Expected | Expected | Expected | Expected | Predicted | Expected |
| Sheep | Expected | Expected | Expected | Expected | Expected | Predicted | Expected |
| Snail | Expected | Expected | Expected | Expected | Expected | Predicted | Expected |
| Xenopus laevis | Expected | Expected | Expected | Expected | Expected | Predicted | Expected |
Dot
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Guinea pig, Pig, Hamster, Snail, Carp, Xenopus laevis, Chicken, Human, Dog, Cow, Goat, Sheep, Rabbit, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes 1/500 using cultured Human or chicken fibroblast extract. Predicted molecular weight: 42 kDa. |
Species Rat | Dilution info - | Notes 1/500 using cultured Human or chicken fibroblast extract. Predicted molecular weight: 42 kDa. |
Species Guinea pig | Dilution info - | Notes 1/500 using cultured Human or chicken fibroblast extract. Predicted molecular weight: 42 kDa. |
Species Pig | Dilution info - | Notes 1/500 using cultured Human or chicken fibroblast extract. Predicted molecular weight: 42 kDa. |
Species Hamster | Dilution info - | Notes 1/500 using cultured Human or chicken fibroblast extract. Predicted molecular weight: 42 kDa. |
Species Snail | Dilution info - | Notes 1/500 using cultured Human or chicken fibroblast extract. Predicted molecular weight: 42 kDa. |
Species Carp | Dilution info - | Notes 1/500 using cultured Human or chicken fibroblast extract. Predicted molecular weight: 42 kDa. |
Species Xenopus laevis | Dilution info - | Notes 1/500 using cultured Human or chicken fibroblast extract. Predicted molecular weight: 42 kDa. |
Species Chicken | Dilution info - | Notes 1/500 using cultured Human or chicken fibroblast extract. Predicted molecular weight: 42 kDa. |
Species Human | Dilution info - | Notes 1/500 using cultured Human or chicken fibroblast extract. Predicted molecular weight: 42 kDa. |
Species Dog | Dilution info - | Notes 1/500 using cultured Human or chicken fibroblast extract. Predicted molecular weight: 42 kDa. |
Species Cow | Dilution info - | Notes 1/500 using cultured Human or chicken fibroblast extract. Predicted molecular weight: 42 kDa. |
Species Goat | Dilution info - | Notes 1/500 using cultured Human or chicken fibroblast extract. Predicted molecular weight: 42 kDa. |
Species Sheep | Dilution info - | Notes 1/500 using cultured Human or chicken fibroblast extract. Predicted molecular weight: 42 kDa. |
Species Rabbit | Dilution info - | Notes 1/500 using cultured Human or chicken fibroblast extract. Predicted molecular weight: 42 kDa. |
IHC-FoFr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Guinea pig, Pig, Hamster, Snail, Carp, Xenopus laevis, Chicken, Human, Dog, Cow, Goat, Sheep, Rabbit | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Guinea pig, Pig, Hamster, Snail, Carp, Xenopus laevis, Chicken, Human, Dog, Cow, Goat, Sheep, Rabbit | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes 1/200 determined by indirect immunofluorescent staining of cultured Human or chicken fibroblasts. |
Species Rat | Dilution info - | Notes 1/200 determined by indirect immunofluorescent staining of cultured Human or chicken fibroblasts. |
Species Guinea pig | Dilution info - | Notes 1/200 determined by indirect immunofluorescent staining of cultured Human or chicken fibroblasts. |
Species Pig | Dilution info - | Notes 1/200 determined by indirect immunofluorescent staining of cultured Human or chicken fibroblasts. |
Species Hamster | Dilution info - | Notes 1/200 determined by indirect immunofluorescent staining of cultured Human or chicken fibroblasts. |
Species Snail | Dilution info - | Notes 1/200 determined by indirect immunofluorescent staining of cultured Human or chicken fibroblasts. |
Species Carp | Dilution info - | Notes 1/200 determined by indirect immunofluorescent staining of cultured Human or chicken fibroblasts. |
Species Xenopus laevis | Dilution info - | Notes 1/200 determined by indirect immunofluorescent staining of cultured Human or chicken fibroblasts. |
Species Chicken | Dilution info - | Notes 1/200 determined by indirect immunofluorescent staining of cultured Human or chicken fibroblasts. |
Species Human | Dilution info - | Notes 1/200 determined by indirect immunofluorescent staining of cultured Human or chicken fibroblasts. |
Species Dog | Dilution info - | Notes 1/200 determined by indirect immunofluorescent staining of cultured Human or chicken fibroblasts. |
Species Cow | Dilution info - | Notes 1/200 determined by indirect immunofluorescent staining of cultured Human or chicken fibroblasts. |
Species Goat | Dilution info - | Notes 1/200 determined by indirect immunofluorescent staining of cultured Human or chicken fibroblasts. |
Species Sheep | Dilution info - | Notes 1/200 determined by indirect immunofluorescent staining of cultured Human or chicken fibroblasts. |
Species Rabbit | Dilution info - | Notes 1/200 determined by indirect immunofluorescent staining of cultured Human or chicken fibroblasts. |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Pig, Xenopus laevis, Rat, Snail, Rabbit, Dog, Mouse, Chicken, Hamster, Guinea pig, Cow, Carp, Goat | Dilution info - | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Guinea pig, Pig, Hamster, Snail, Carp, Xenopus laevis, Chicken, Human, Dog, Cow, Goat, Sheep, Rabbit | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Alternative names
ACTA, ACTA1, Alpha-actin-1
Recommended products
Mouse Monoclonal alpha skeletal muscle Actin antibody. Suitable for Dot, WB, IHC-FoFr, IHC-P, ICC/IF, IHC-Fr, ELISA and reacts with Guinea pig, Pig, Hamster, Snail, Carp, Xenopus laevis, Chicken, Human, Dog, Cow, Goat, Sheep, Rabbit, Mouse, Rat samples. Cited in 52 publications. Immunogen corresponding to Synthetic Peptide within Human ACTA1 aa 350 to C-terminus.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human ACTA1 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P68133
- Clone number
- AC-40
- Purity
- Tissue culture supernatant
- Epitope
- Monoclonal anti-Actin recognizes an epitope located on the C-terminal end of actin. This epitope is conserved in all actin isoforms.
- Concentration
- Purification notes
Purified from TCS.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
This product was changed from ascites to tissue culture supernatant on 21 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Actin also known as globular (G-actin) or filamentous (F-actin) actin plays a central role in cell structure and movement. This protein has a molecular weight of approximately 42 kDa and resides abundantly in eukaryotic cells especially in muscle and cytoskeletal structures. It comes in several isoforms with varying expression profiles across tissues. Actin can undergo polymerization from its G-actin monomers into F-actin filaments a process that is reversible through depolymerization allowing for dynamic cellular functions.
- Biological function summary
Actin contributes to various cellular processes by forming the cytoskeleton which provides mechanical support and determines cell shape. Actin also facilitates cell motility division and intracellular transport through rapid polymerization and depolymerization cycles. Within cells actin associates with other proteins to form complexes such as the Arp2/3 complex which assists actin in the branching of filaments critical for pushing forward the cell's leading edge during movement. Techniques like actin immunofluorescence help visualize actin filaments within cells revealing its extensive network.
- Pathways
Actin plays an integral role in pathways like cell movement and signal transduction. The Rho family of GTPases regulates actin cytoskeleton rearrangements influencing cell shape and migration. Actin function interactions involve proteins like myosin forming actomyosin complexes essential for muscle contraction and other cell motility activities. Actin polymerization and depolymerization cycles are key to the dynamic regulation within these pathways ensuring adequate cellular responses to environmental signals.
- Associated diseases and disorders
Mutations or misregulation of actin and its associated pathways link to conditions such as cardiomyopathies and cancer metastasis. In familial cardiomyopathy actin mutations disrupt normal cardiac muscle contraction. In cancer altered actin polymerization and depolymerization enable invasive cell migration facilitating metastasis. Proteins like myosin and tropomyosin associate with actin in these pathologies illustrating the impact of actin dynamics on disease progression and highlighting actin as a therapeutic target. Anti-actin antibodies can be used in research and diagnostics to better understand these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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1 product image
![Immunohistochemistry (Frozen sections) - Anti-Actin antibody [AC-40] - Loading Control (ab11003), expandable thumbnail](https://content.abcam.com/products/images/actin-antibody-ac-40-loading-control-ab11003--immunohistochemistry-frozen-sections-img107832.jpg "Immunohistochemistry (Frozen sections) - Anti-Actin antibody [AC-40] - Loading Control (ab11003)")
Immunohistochemistry (Frozen sections) - Anti-Actin antibody [AC-40] - Loading Control (ab11003)
Actin Immunohistochemistry (Frozen sections) staining using mouse Anti-Actin antibody
Immunohistochemical analysis of frozen Human tongue tissue, using ab11003.
This image was generated using the ascites version of the product.
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