AB198991

Anti-Actin antibody [EPR16770] - Loading Control

RabMAb
Recombinant
Lab Essentials
20ul selling size
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(10 Publications)

Rabbit Recombinant Monoclonal alpha skeletal muscle Actin antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 10 publications.

View Alternative Names

ACTA, ACTA1, Alpha-actin-1

4 Images

Supplier Data

Immunoprecipitation - Anti-Actin antibody [EPR16770] - Loading Control (AB198991)

Actin was immunoprecipitated from 1mg of 293 (Human embryonic kidney) whole cell extract with ab198991 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab198991 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : 293 whole cell extract 10 μg (Input). Lane 2 : ab198991 IP in 293 whole cell extract. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab198991 in 293 whole cell extract.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Actin antibody [EPR16770] - Loading Control (ab198991)

Predicted band size: 42 kDa

false

Supplier Data

Western blot - Anti-Actin antibody [EPR16770] - Loading Control (AB198991)

Blocking/Dilution buffer : 5% NFDM/TBST.

This antibody can recognize ACTS, ACTH, ACTG, ACTC, ACTB & ACTA, it is a pan Actin.

All lanes:

Western blot - Anti-Actin antibody [EPR16770] - Loading Control (ab198991) at 1/20000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

Human fetal heart lysate at 10 µg

Lane 3:

Human fetal kidney lysate at 10 µg

Lane 4:

Human fetal spleen lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

false

Exposure time: 15s

Supplier Data

Western blot - Anti-Actin antibody [EPR16770] - Loading Control (AB198991)

Blocking/Dilution buffer : 5% NFDM/TBST.

This antibody can recognize ACTS, ACTH, ACTG, ACTC, ACTB & ACTA, it is a pan Actin.

All lanes:

Western blot - Anti-Actin antibody [EPR16770] - Loading Control (ab198991) at 1/20000 dilution

Lane 1:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 2:

293 (Human embryonic kidney) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

false

Exposure time: 15s

Supplier Data

Western blot - Anti-Actin antibody [EPR16770] - Loading Control (AB198991)

Blocking/Dilution buffer : 5% NFDM/TBST.

This antibody can recognize ACTS, ACTH, ACTG, ACTC, ACTB & ACTA, it is a pan Actin.

All lanes:

Western blot - Anti-Actin antibody [EPR16770] - Loading Control (ab198991) at 1/20000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Mouse heart lysate at 10 µg

Lane 3:

Mouse kidney lysate at 10 µg

Lane 4:

Mouse spleen lysate at 10 µg

Lane 5:

Rat brain lysate at 10 µg

Lane 6:

Rat heart lysate at 10 µg

Lane 7:

Rat kidney lysate at 10 µg

Lane 8:

Rat spleen lysate at 10 µg

Lane 9:

C6 (Rat glial tumor cells) whole cell lysate at 10 µg

Lane 10:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 11:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

Lane 12:

NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

false

Exposure time: 15s

Carrier free

Anti-Actin antibody [EPR16770] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16770

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/20000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/20000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/20000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Actin also known as globular (G-actin) or filamentous (F-actin) actin plays a central role in cell structure and movement. This protein has a molecular weight of approximately 42 kDa and resides abundantly in eukaryotic cells especially in muscle and cytoskeletal structures. It comes in several isoforms with varying expression profiles across tissues. Actin can undergo polymerization from its G-actin monomers into F-actin filaments a process that is reversible through depolymerization allowing for dynamic cellular functions.

Biological function summary

Actin contributes to various cellular processes by forming the cytoskeleton which provides mechanical support and determines cell shape. Actin also facilitates cell motility division and intracellular transport through rapid polymerization and depolymerization cycles. Within cells actin associates with other proteins to form complexes such as the Arp2/3 complex which assists actin in the branching of filaments critical for pushing forward the cell's leading edge during movement. Techniques like actin immunofluorescence help visualize actin filaments within cells revealing its extensive network.

Pathways

Actin plays an integral role in pathways like cell movement and signal transduction. The Rho family of GTPases regulates actin cytoskeleton rearrangements influencing cell shape and migration. Actin function interactions involve proteins like myosin forming actomyosin complexes essential for muscle contraction and other cell motility activities. Actin polymerization and depolymerization cycles are key to the dynamic regulation within these pathways ensuring adequate cellular responses to environmental signals.

Mutations or misregulation of actin and its associated pathways link to conditions such as cardiomyopathies and cancer metastasis. In familial cardiomyopathy actin mutations disrupt normal cardiac muscle contraction. In cancer altered actin polymerization and depolymerization enable invasive cell migration facilitating metastasis. Proteins like myosin and tropomyosin associate with actin in these pathologies illustrating the impact of actin dynamics on disease progression and highlighting actin as a therapeutic target. Anti-actin antibodies can be used in research and diagnostics to better understand these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

See full target information ACTA1

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 29:e70800 PubMed40845179

2025

Decoy Receptor 2 as a Cell Cycle Arrest Biomarker for Predicting Renal Recovery Following Acute Kidney Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangling Yi,Liming Wang,Xiao-Yue Wang,Yu Fang,Jiarui Liu,Kehong Chen,Ya-Ni He,Jia Chen

Immunology 176:132-144 PubMed40503724

2025

PD-1 Regulates the Glycolytic Pathway to Reverse Abnormal CD4 T Cell Differentiation and Alleviate Hashimoto's Thyroiditis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Jiang,Tao Luo,Xinyu Zhao,Pengqian Li,Xiaotong Gu,Chuchu Wan,Xingjie Xie,Haixia Liu

Epilepsia open 9:2173-2185 PubMed39501495

2024

Acute depletion of complement C3 with cobra venom factor attenuates memory deficits induced by status epilepticus.

Applications

Unspecified application

Species

Unspecified reactive species

Nicole D Schartz,Yibo Li,Alexandra L Sommer,Amy L Brewster

Environment & health (Washington, D.C.) 2:563-571 PubMed39474290

2024

Extreme Heat Exposure Induced Acute Kidney Injury through NLRP3 Inflammasome Activation in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jiahui Wang,Kunyi Wang,Xinnu Shi,Zhoufan Hu,Lisha Zhao,Kan Chen,Lu Zhang,Ran Li,Guoqing Zhang,Cuiqing Liu

Molecular medicine (Cambridge, Mass.) 30:173 PubMed39390372

2024

Drp1 acetylation mediated by CDK5-AMPK-GCN5L1 axis promotes cerebral ischemic injury via facilitating mitochondrial fission.

Applications

Unspecified application

Species

Unspecified reactive species

Jiejie Zhang,Shan Wang,Haitao Zhang,Xiaotong Yang,Xin Ren,Lei Wang,Yihan Yang,Yi Yang,Ya Wen

Nature communications 14:8282 PubMed38092772

2023

Structural variants involved in high-altitude adaptation detected using single-molecule long-read sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Jinlong Shi,Zhilong Jia,Jinxiu Sun,Xiaoreng Wang,Xiaojing Zhao,Chenghui Zhao,Fan Liang,Xinyu Song,Jiawei Guan,Xue Jia,Jing Yang,Qi Chen,Kang Yu,Qian Jia,Jing Wu,Depeng Wang,Yuhui Xiao,Xiaoman Xu,Yinzhe Liu,Shijing Wu,Qin Zhong,Jue Wu,Saijia Cui,Xiaochen Bo,Zhenzhou Wu,Minsung Park,Manolis Kellis,Kunlun He

Frontiers in molecular neuroscience 16:1265944 PubMed38035266

2023

Mice deficient in complement C3 are protected against recognition memory deficits and astrogliosis induced by status epilepticus.

Applications

Unspecified application

Species

Unspecified reactive species

Nicole D Schartz,Alisha Aroor,Yibo Li,Nicole Pinzón-Hoyos,Amy L Brewster

Aging cell 22:e13958 PubMed37614147

2023

Inhibition of abnormal C/EBPβ/α-Syn signaling pathway through activation of Nrf2 ameliorates Parkinson's disease-like pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Zefang Lin,Lixuan Huang,Qianqian Cao,Hanyue Luo,Wei Yao,Ji-Chun Zhang

Molecular therapy : the journal of the American Society of Gene Therapy 27:188-199 PubMed30449662

2018

Development of Novel DNA-Encoded PCSK9 Monoclonal Antibodies as Lipid-Lowering Therapeutics.

Applications

Unspecified application

Species

Unspecified reactive species

Makan Khoshnejad,Ami Patel,Krzysztof Wojtak,Sagar B Kudchodkar,Laurent Humeau,Nicholas N Lyssenko,Daniel J Rader,Kar Muthumani,David B Weiner

OncoTargets and therapy 9:1067-75 PubMed27042099

2016

SPAG9 is involved in hepatocarcinoma cell migration and invasion via modulation of ELK1 expression.

Applications

Species

Unspecified reactive species

Qiuyue Yan,Guohua Lou,Ying Qian,Bo Qin,Xiuping Xu,Yanan Wang,Yanning Liu,Xuejun Dong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Actin antibody [EPR16770] - Loading Control

Immunoprecipitation - Anti-Actin antibody [EPR16770] - Loading Control (AB198991)

Western blot - Anti-Actin antibody [EPR16770] - Loading Control (AB198991)

Western blot - Anti-Actin antibody [EPR16770] - Loading Control (AB198991)

Western blot - Anti-Actin antibody [EPR16770] - Loading Control (AB198991)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

Product promise