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Rabbit Recombinant Monoclonal alpha skeletal muscle Actin antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

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Images

Western blot - Anti-Actin antibody [EPR16770] - Loading Control (AB198991), expandable thumbnail
  • Western blot - Anti-Actin antibody [EPR16770] - Loading Control (AB198991), expandable thumbnail
  • Immunoprecipitation - Anti-Actin antibody [EPR16770] - Loading Control (AB198991), expandable thumbnail
  • Western blot - Anti-Actin antibody [EPR16770] - Loading Control (AB198991), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested
Mouse
Expected
Tested
Rat
Expected
Tested

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/20000
Notes

-

Species
Rat
Dilution info
1/20000
Notes

-

Species
Human
Dilution info
1/20000
Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Alternative names

ACTA, ACTA1, Alpha-actin-1

Recommended products

Rabbit Recombinant Monoclonal alpha skeletal muscle Actin antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR16770
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Actin also known as globular (G-actin) or filamentous (F-actin) actin plays a central role in cell structure and movement. This protein has a molecular weight of approximately 42 kDa and resides abundantly in eukaryotic cells especially in muscle and cytoskeletal structures. It comes in several isoforms with varying expression profiles across tissues. Actin can undergo polymerization from its G-actin monomers into F-actin filaments a process that is reversible through depolymerization allowing for dynamic cellular functions.

Biological function summary

Actin contributes to various cellular processes by forming the cytoskeleton which provides mechanical support and determines cell shape. Actin also facilitates cell motility division and intracellular transport through rapid polymerization and depolymerization cycles. Within cells actin associates with other proteins to form complexes such as the Arp2/3 complex which assists actin in the branching of filaments critical for pushing forward the cell's leading edge during movement. Techniques like actin immunofluorescence help visualize actin filaments within cells revealing its extensive network.

Pathways

Actin plays an integral role in pathways like cell movement and signal transduction. The Rho family of GTPases regulates actin cytoskeleton rearrangements influencing cell shape and migration. Actin function interactions involve proteins like myosin forming actomyosin complexes essential for muscle contraction and other cell motility activities. Actin polymerization and depolymerization cycles are key to the dynamic regulation within these pathways ensuring adequate cellular responses to environmental signals.

Associated diseases and disorders

Mutations or misregulation of actin and its associated pathways link to conditions such as cardiomyopathies and cancer metastasis. In familial cardiomyopathy actin mutations disrupt normal cardiac muscle contraction. In cancer altered actin polymerization and depolymerization enable invasive cell migration facilitating metastasis. Proteins like myosin and tropomyosin associate with actin in these pathologies illustrating the impact of actin dynamics on disease progression and highlighting actin as a therapeutic target. Anti-actin antibodies can be used in research and diagnostics to better understand these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Actin antibody [EPR16770] - Loading Control (ab198991), expandable thumbnail

    Western blot - Anti-Actin antibody [EPR16770] - Loading Control (ab198991)

    Actin Western blot staining using rabbit Anti-Actin antibody

    Blocking/Dilution buffer: 5% NFDM/TBST.

    This antibody can recognize ACTS, ACTH, ACTG, ACTC, ACTB & ACTA, it is a pan Actin.

    All lanes: Western blot - Anti-Actin antibody [EPR16770] - Loading Control (ab198991) at 1/20000 dilution

    Lane 1: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg

    Lane 2: 293 (Human embryonic kidney) whole cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa

    Observed band size: 42 kDa

    Exposure time: 15s

  • Western blot - Anti-Actin antibody [EPR16770] - Loading Control (ab198991), expandable thumbnail

    Western blot - Anti-Actin antibody [EPR16770] - Loading Control (ab198991)

    Actin Western blot staining using rabbit Anti-Actin antibody

    Blocking/Dilution buffer: 5% NFDM/TBST.

    This antibody can recognize ACTS, ACTH, ACTG, ACTC, ACTB & ACTA, it is a pan Actin.

    All lanes: Western blot - Anti-Actin antibody [EPR16770] - Loading Control (ab198991) at 1/20000 dilution

    Lane 1: Human fetal brain lysate at 10 µg

    Lane 2: Human fetal heart lysate at 10 µg

    Lane 3: Human fetal kidney lysate at 10 µg

    Lane 4: Human fetal spleen lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 42 kDa

    Observed band size: 42 kDa

    Exposure time: 15s

  • Immunoprecipitation - Anti-Actin antibody [EPR16770] - Loading Control (ab198991), expandable thumbnail

    Immunoprecipitation - Anti-Actin antibody [EPR16770] - Loading Control (ab198991)

    Actin was immunoprecipitated from 1mg of 293 (Human embryonic kidney) whole cell extract with ab198991 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab198991 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: 293 whole cell extract 10 μg (Input). Lane 2: ab198991 IP in 293 whole cell extract. Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab198991 in 293 whole cell extract.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-Actin antibody [EPR16770] - Loading Control (ab198991)

    Predicted band size: 42 kDa

  • Western blot - Anti-Actin antibody [EPR16770] - Loading Control (ab198991), expandable thumbnail

    Western blot - Anti-Actin antibody [EPR16770] - Loading Control (ab198991)

    Actin Western blot staining using rabbit Anti-Actin antibody

    Blocking/Dilution buffer: 5% NFDM/TBST.

    This antibody can recognize ACTS, ACTH, ACTG, ACTC, ACTB & ACTA, it is a pan Actin.

    All lanes: Western blot - Anti-Actin antibody [EPR16770] - Loading Control (ab198991) at 1/20000 dilution

    Lane 1: Mouse brain lysate at 10 µg

    Lane 2: Mouse heart lysate at 10 µg

    Lane 3: Mouse kidney lysate at 10 µg

    Lane 4: Mouse spleen lysate at 10 µg

    Lane 5: Rat brain lysate at 10 µg

    Lane 6: Rat heart lysate at 10 µg

    Lane 7: Rat kidney lysate at 10 µg

    Lane 8: Rat spleen lysate at 10 µg

    Lane 9: C6 (Rat glial tumor cells) whole cell lysate at 10 µg

    Lane 10: RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

    Lane 11: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

    Lane 12: NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa

    Observed band size: 42 kDa

    Exposure time: 15s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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