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Rabbit Recombinant Monoclonal alpha skeletal muscle Actin antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Chicken, Human samples. Cited in 5 publications.

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Images

Western blot - Anti-Actin antibody [EPR16875] - Loading Control (AB200658), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [EPR16875] (AB200658), expandable thumbnail
  • Western blot - Anti-Actin antibody [EPR16875] - Loading Control (AB200658), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Actin antibody [EPR16875] - Loading Control (AB200658), expandable thumbnail
  • Western blot - Anti-Actin antibody [EPR16875] - Loading Control (AB200658), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Mouse
Tested
Expected
Expected
Rat
Tested
Expected
Expected
Chicken
Tested
Expected
Expected

Tested
Tested

Species
Mouse
Dilution info
1/2000
Notes

-

Species
Rat
Dilution info
1/2000
Notes

-

Species
Chicken
Dilution info
1/2000
Notes

-

Species
Human
Dilution info
1/2000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Mouse, Rat, Chicken
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Expected
Expected

Species
Mouse, Rat, Chicken
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Alternative names

ACTA, ACTA1, Alpha-actin-1

Recommended products

Rabbit Recombinant Monoclonal alpha skeletal muscle Actin antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Chicken, Human samples. Cited in 5 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR16875
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Actin also known as globular (G-actin) or filamentous (F-actin) actin plays a central role in cell structure and movement. This protein has a molecular weight of approximately 42 kDa and resides abundantly in eukaryotic cells especially in muscle and cytoskeletal structures. It comes in several isoforms with varying expression profiles across tissues. Actin can undergo polymerization from its G-actin monomers into F-actin filaments a process that is reversible through depolymerization allowing for dynamic cellular functions.

Biological function summary

Actin contributes to various cellular processes by forming the cytoskeleton which provides mechanical support and determines cell shape. Actin also facilitates cell motility division and intracellular transport through rapid polymerization and depolymerization cycles. Within cells actin associates with other proteins to form complexes such as the Arp2/3 complex which assists actin in the branching of filaments critical for pushing forward the cell's leading edge during movement. Techniques like actin immunofluorescence help visualize actin filaments within cells revealing its extensive network.

Pathways

Actin plays an integral role in pathways like cell movement and signal transduction. The Rho family of GTPases regulates actin cytoskeleton rearrangements influencing cell shape and migration. Actin function interactions involve proteins like myosin forming actomyosin complexes essential for muscle contraction and other cell motility activities. Actin polymerization and depolymerization cycles are key to the dynamic regulation within these pathways ensuring adequate cellular responses to environmental signals.

Associated diseases and disorders

Mutations or misregulation of actin and its associated pathways link to conditions such as cardiomyopathies and cancer metastasis. In familial cardiomyopathy actin mutations disrupt normal cardiac muscle contraction. In cancer altered actin polymerization and depolymerization enable invasive cell migration facilitating metastasis. Proteins like myosin and tropomyosin associate with actin in these pathologies illustrating the impact of actin dynamics on disease progression and highlighting actin as a therapeutic target. Anti-actin antibodies can be used in research and diagnostics to better understand these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-Actin antibody [EPR16875] - Loading Control (ab200658), expandable thumbnail

    Western blot - Anti-Actin antibody [EPR16875] - Loading Control (ab200658)

    Actin Western blot staining using rabbit Anti-Actin antibody

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Actin antibody [EPR16875] - Loading Control (ab200658) at 1/2000 dilution

    Lane 1: Mouse brain lysate at 10 µg

    Lane 2: Mouse heart lysate at 10 µg

    Lane 3: Mouse kidney lysate at 10 µg

    Lane 4: Mouse spleen lysate at 10 µg

    Lane 5: Rat brain lysate at 10 µg

    Lane 6: Rat heart lysate at 10 µg

    Lane 7: Rat kidney lysate at 10 µg

    Lane 8: C6 (Rat glial tumor cells) cell lysate at 10 µg

    Lane 9: RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa

    Observed band size: 42 kDa

    Exposure time: 5s

  • Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [EPR16875] (ab200658), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [EPR16875] (ab200658)

    Immunofluorescent analysis of 100% Methanol-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Actin with ab200658 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on HeLa cell line is observed.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab200658 at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Western blot - Anti-Actin antibody [EPR16875] - Loading Control (ab200658), expandable thumbnail

    Western blot - Anti-Actin antibody [EPR16875] - Loading Control (ab200658)

    Actin Western blot staining using rabbit Anti-Actin antibody

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Actin antibody [EPR16875] - Loading Control (ab200658) at 1/10000 dilution

    Lane 1: Jurkat (Human T cell leukemia cells from peripheral blood) cell lysate at 10 µg

    Lane 2: HepG2 (Human liver hepatocellular carcinoma) cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa

    Observed band size: 42 kDa

    Exposure time: 3min

  • Flow Cytometry (Intracellular) - Anti-Actin antibody [EPR16875] - Loading Control (ab200658), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Actin antibody [EPR16875] - Loading Control (ab200658)

    Actin Flow Cytometry (Intracellular) staining using rabbit Anti-Actin antibody

    Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Actin with ab200658 at 1/100 dilution (red) compared with a rabbit monoclonal IgG isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

  • Western blot - Anti-Actin antibody [EPR16875] - Loading Control (ab200658), expandable thumbnail

    Western blot - Anti-Actin antibody [EPR16875] - Loading Control (ab200658)

    Actin Western blot staining of HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate using rabbit Anti-Actin antibody

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Actin antibody [EPR16875] - Loading Control (ab200658) at 1/2000 dilution

    All lanes: HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa

    Observed band size: 42 kDa

    Exposure time: 10s

  • Western blot - Anti-Actin antibody [EPR16875] - Loading Control (ab200658), expandable thumbnail

    Western blot - Anti-Actin antibody [EPR16875] - Loading Control (ab200658)

    Actin Western blot staining of UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) cell lysate using rabbit Anti-Actin antibody

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Actin antibody [EPR16875] - Loading Control (ab200658) at 1/2000 dilution

    All lanes: UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa

    Observed band size: 42 kDa

    Exposure time: 5s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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