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Rabbit Recombinant Monoclonal alpha skeletal muscle Actin antibody. Carrier free. Suitable for IP, WB and reacts with Mouse, Rat, Human, Recombinant full length protein - Human samples.

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Images

Western blot - Anti-Actin antibody [EPR19353-16] - BSA and Azide free (AB251552), expandable thumbnail
  • Western blot - Anti-Actin antibody [EPR19353-16] - BSA and Azide free (AB251552), expandable thumbnail
  • Western blot - Anti-Actin antibody [EPR19353-16] - BSA and Azide free (AB251552), expandable thumbnail
  • Immunoprecipitation - Anti-Actin antibody [EPR19353-16] - BSA and Azide free (AB251552), expandable thumbnail
  • Western blot - Anti-Actin antibody [EPR19353-16] - BSA and Azide free (AB251552), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Expected
Tested
Mouse
Tested
Tested
Rat
Expected
Tested
Recombinant full length protein - Human
Not recommended
Tested

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Rat, Human, Mouse, Recombinant full length protein - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Alternative names

ACTA, ACTA1, Alpha-actin-1

Recommended products

Rabbit Recombinant Monoclonal alpha skeletal muscle Actin antibody. Carrier free. Suitable for IP, WB and reacts with Mouse, Rat, Human, Recombinant full length protein - Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR19353-16
Specificity

This is a pan-actin antibody. It will detect all 6 actin isoforms corresponding to SwissProt IDs P63261, P60709, P68032, P63267, P62736 and P68133.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab251552 is the carrier-free version of Anti-Actin antibody [EPR19353-16] ab216070.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Actin also known as globular (G-actin) or filamentous (F-actin) actin plays a central role in cell structure and movement. This protein has a molecular weight of approximately 42 kDa and resides abundantly in eukaryotic cells especially in muscle and cytoskeletal structures. It comes in several isoforms with varying expression profiles across tissues. Actin can undergo polymerization from its G-actin monomers into F-actin filaments a process that is reversible through depolymerization allowing for dynamic cellular functions.

Biological function summary

Actin contributes to various cellular processes by forming the cytoskeleton which provides mechanical support and determines cell shape. Actin also facilitates cell motility division and intracellular transport through rapid polymerization and depolymerization cycles. Within cells actin associates with other proteins to form complexes such as the Arp2/3 complex which assists actin in the branching of filaments critical for pushing forward the cell's leading edge during movement. Techniques like actin immunofluorescence help visualize actin filaments within cells revealing its extensive network.

Pathways

Actin plays an integral role in pathways like cell movement and signal transduction. The Rho family of GTPases regulates actin cytoskeleton rearrangements influencing cell shape and migration. Actin function interactions involve proteins like myosin forming actomyosin complexes essential for muscle contraction and other cell motility activities. Actin polymerization and depolymerization cycles are key to the dynamic regulation within these pathways ensuring adequate cellular responses to environmental signals.

Associated diseases and disorders

Mutations or misregulation of actin and its associated pathways link to conditions such as cardiomyopathies and cancer metastasis. In familial cardiomyopathy actin mutations disrupt normal cardiac muscle contraction. In cancer altered actin polymerization and depolymerization enable invasive cell migration facilitating metastasis. Proteins like myosin and tropomyosin associate with actin in these pathologies illustrating the impact of actin dynamics on disease progression and highlighting actin as a therapeutic target. Anti-actin antibodies can be used in research and diagnostics to better understand these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Actin antibody [EPR19353-16] - BSA and Azide free (ab251552), expandable thumbnail

    Western blot - Anti-Actin antibody [EPR19353-16] - BSA and Azide free (ab251552)

    This data was developed using Anti-Actin antibody [EPR19353-16] ab216070, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Human Gamma actin (ACTG1) contains aa2-182 with His-tag. Human Beta actin (ACTB) contains aa1-100 with GST and His tag. Human Gamma-2 actin (ACTG2) contains aa1-200 with His-tag. Human Alpha-cardiac actin (ACTC) contains aa1-200 with His-tag. Human Alpha-actin-2 (ACTA2) contains aa3-253 with His-tag. Human Alpha-actin-1 (ACTA1) contains aa3-200 with His-tag.

    All lanes: Western blot - Anti-Actin antibody [EPR19353-16] (Anti-Actin antibody [EPR19353-16] ab216070) at 1/1000 dilution

    Lane 1: Human Gamma actin (ACTG1) recombinant protein at 0.01 µg

    Lane 2: Human Beta actin (ACTB) recombinant protein at 0.02 µg

    Lane 3: Human Gamma-2 actin (ACTG2) recombinant protein at 0.02 µg

    Lane 4: Human Alpha-cardiac actin (ACTC) recombinant protein at 0.02 µg

    Lane 5: Human Alpha-actin-2 (ACTA2) recombinant protein at 0.01 µg

    Lane 6: Human Alpha-actin-1 (ACTA1) recombinant protein at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 42 kDa

    Exposure time: 1min

  • Western blot - Anti-Actin antibody [EPR19353-16] - BSA and Azide free (ab251552), expandable thumbnail

    Western blot - Anti-Actin antibody [EPR19353-16] - BSA and Azide free (ab251552)

    This data was developed using Anti-Actin antibody [EPR19353-16] ab216070, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Actin antibody [EPR19353-16] (Anti-Actin antibody [EPR19353-16] ab216070) at 1/1000 dilution

    Lane 1: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

    Lane 2: HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

    Lane 3: A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

    Lane 4: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 42 kDa

    Observed band size: 42 kDa

    Exposure time: 3min

  • Western blot - Anti-Actin antibody [EPR19353-16] - BSA and Azide free (ab251552), expandable thumbnail

    Western blot - Anti-Actin antibody [EPR19353-16] - BSA and Azide free (ab251552)

    This data was developed using Anti-Actin antibody [EPR19353-16] ab216070, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Actin antibody [EPR19353-16] (Anti-Actin antibody [EPR19353-16] ab216070) at 1/1000 dilution

    Lane 1: Human skeletal muscle lysate at 10 µg

    Lane 2: Human fetal kidney tissue lysate at 10 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/4000 dilution

    Predicted band size: 42 kDa

    Observed band size: 42 kDa

    Exposure time: 30s

  • Immunoprecipitation - Anti-Actin antibody [EPR19353-16] - BSA and Azide free (ab251552), expandable thumbnail

    Immunoprecipitation - Anti-Actin antibody [EPR19353-16] - BSA and Azide free (ab251552)

    This data was developed using Anti-Actin antibody [EPR19353-16] ab216070, the same antibody clone in a different buffer formulation.Actin was immunoprecipitated from 0.35 mg of NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate with Anti-Actin antibody [EPR19353-16] ab216070 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-Actin antibody [EPR19353-16] ab216070 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1,000 dilution Lane 1: NIH/3T3 whole cell lysate, 10 μg (Input). Lane 2: Anti-Actin antibody [EPR19353-16] ab216070 IP in NIH/3T3 whole cell lysate. Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Actin antibody [EPR19353-16] ab216070 in NIH/3T3 whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 3 minutes. Note: Customer optimization may be required.

    All lanes: Immunoprecipitation - Anti-Actin antibody [EPR19353-16] (Anti-Actin antibody [EPR19353-16] ab216070)

    Predicted band size: 42 kDa

  • Western blot - Anti-Actin antibody [EPR19353-16] - BSA and Azide free (ab251552), expandable thumbnail

    Western blot - Anti-Actin antibody [EPR19353-16] - BSA and Azide free (ab251552)

    This data was developed using Anti-Actin antibody [EPR19353-16] ab216070, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Actin antibody [EPR19353-16] (Anti-Actin antibody [EPR19353-16] ab216070) at 1/1000 dilution

    Lane 1: C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

    Lane 2: PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

    Lane 3: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

    Lane 4: Mouse brain lysate at 10 µg

    Lane 5: Mouse spleen lysate at 10 µg

    Lane 6: Rat spleen lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 42 kDa

    Observed band size: 42 kDa

    Exposure time: 3min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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