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Human Recombinant Monoclonal alpha skeletal muscle Actin antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [IGX3831H] (AB213251), expandable thumbnail
  • Western blot - Anti-Actin antibody [IGX3831H] (AB213251), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [IGX3831H] (AB213251), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Human

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested
Mouse
Tested
Tested

Tested
Tested

Species

Mouse

Dilution info

1-5 µg/mL

Notes

-

Species

Human

Dilution info

1-5 µg/mL

Notes

-

Tested
Tested

Species

Mouse

Dilution info

5 µg/mL

Notes

This product gave a positive signal in HeLa cells and NIH3T3 fixed with 4% formaldehyde

Species

Human

Dilution info

5 µg/mL

Notes

This product gave a positive signal in HeLa cells and NIH3T3 fixed with 4% formaldehyde

Associated Products

Select an associated product type

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Target data

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Alternative names

Recommended products

Human Recombinant Monoclonal alpha skeletal muscle Actin antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

IGX3831H

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product was made using synthetic libraries and phage display technology.

This antibody is a recombinant antibody.
Human monoclonal antibody.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Actin also known as globular (G-actin) or filamentous (F-actin) actin plays a central role in cell structure and movement. This protein has a molecular weight of approximately 42 kDa and resides abundantly in eukaryotic cells especially in muscle and cytoskeletal structures. It comes in several isoforms with varying expression profiles across tissues. Actin can undergo polymerization from its G-actin monomers into F-actin filaments a process that is reversible through depolymerization allowing for dynamic cellular functions.

Biological function summary

Actin contributes to various cellular processes by forming the cytoskeleton which provides mechanical support and determines cell shape. Actin also facilitates cell motility division and intracellular transport through rapid polymerization and depolymerization cycles. Within cells actin associates with other proteins to form complexes such as the Arp2/3 complex which assists actin in the branching of filaments critical for pushing forward the cell's leading edge during movement. Techniques like actin immunofluorescence help visualize actin filaments within cells revealing its extensive network.

Pathways

Actin plays an integral role in pathways like cell movement and signal transduction. The Rho family of GTPases regulates actin cytoskeleton rearrangements influencing cell shape and migration. Actin function interactions involve proteins like myosin forming actomyosin complexes essential for muscle contraction and other cell motility activities. Actin polymerization and depolymerization cycles are key to the dynamic regulation within these pathways ensuring adequate cellular responses to environmental signals.

Associated diseases and disorders

Mutations or misregulation of actin and its associated pathways link to conditions such as cardiomyopathies and cancer metastasis. In familial cardiomyopathy actin mutations disrupt normal cardiac muscle contraction. In cancer altered actin polymerization and depolymerization enable invasive cell migration facilitating metastasis. Proteins like myosin and tropomyosin associate with actin in these pathologies illustrating the impact of actin dynamics on disease progression and highlighting actin as a therapeutic target. Anti-actin antibodies can be used in research and diagnostics to better understand these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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3 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [IGX3831H] (ab213251), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [IGX3831H] (ab213251)

    ab213251 staining Actin in NIH3T3 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab213251 at a 5μg/ml concentration, then detected with a goat anti-human (Alexa Fluor® 488) secondary antibody at a 1/2000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Western blot - Anti-Actin antibody [IGX3831H] (ab213251), expandable thumbnail

    Western blot - Anti-Actin antibody [IGX3831H] (ab213251)

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab213251 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

    All lanes: Anti-Actin [IGX3831H] at 1 µg/mL

    Lane 1: Human colon tissue lysate at 10 µg

    Lane 2: Mouse colon tissue lysate at 10 µg

    Lane 3: HeLa whole cell lysate at 10 µg

    Lane 4: Jurkat whole cell lysate at 10 µg

    Lane 5: NIH3T3 whole cell lysate at 10 µg

    Lane 6: PANC1 whole cell lysate at 10 µg

    Lane 7: C2C12 whole cell lysate at 10 µg

    Lane 8: Human skeletal muscle tissue lysate at 10 µg

    Secondary

    All lanes: HRP conjugated Goat Anti-Human IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 42 kDa

    Exposure time: 1min

  • Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [IGX3831H] (ab213251), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [IGX3831H] (ab213251)

    ab213251 staining Actin in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab213251 at a 5μg/ml concentration, then detected with a goat anti-human (Alexa Fluor® 488) secondary antibody at a 1/2000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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