Anti-Actin antibody [IGX3831R-3] - Loading Control

3 product images

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  Western blot - Anti-Actin antibody [IGX3831R-3] - Loading Control (ab218787)

  Actin Western blot staining using rabbit Anti-Actin antibody

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab218787 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Anti-Actin [IGX3831R-3] at 1 µg/mL

  Lane 1: Human colon tissue lysate at 10 µg

  Lane 2: Mouse colon tissue lysate at 10 µg

  Lane 3: HeLa whole cell lysate at 10 µg

  Lane 4: Jurkat whole cell lysate at 10 µg

  Lane 5: NIH3T3 whole cell lysate at 10 µg

  Lane 6: PANC1 whole cell lysate at 10 µg

  Lane 7: C2C12 whole cell lysate at 10 µg

  Lane 8: Human skeletal muscle tissue lysate at 10 µg

  Secondary

  All lanes: Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 42 kDa

  Exposure time: 2min

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  Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [IGX3831R-3] - Loading Control (ab218787)

  Actin Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-Actin antibody

  ab218787 staining Actin in NIH3T3 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab218787 at a 5μg/ml concentration, then detected with a goat anti-human (Alexa Fluor^® 488) secondary antibody at a 1/2000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
  

  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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  Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [IGX3831R-3] (ab218787)

  ab218787 staining Actin in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab218787 at a 5μg/ml concentration, then detected with a goat anti-human (Alexa Fluor^® 488) secondary antibody at a 1/2000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
  

  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).