AB197345

Anti-Actin antibody - Loading Control

Lab Essentials
What is this?

Be the first to review this product! Submit a review

(30 Publications)

Rabbit Polyclonal ACT12 antibody. Suitable for WB and reacts with Arabidopsis thaliana, Fava Bean, Cinnamomum camphora, Wheat, Brassica rapa, Tobacco samples. Cited in 30 publications. Immunogen corresponding to Synthetic Peptide within Arabidopsis thaliana ACT12.

View Alternative Names

At3g46520, F12A12.40, ACT12, Actin-12

1 Images

Supplier Data

Western blot - Anti-Actin antibody - Loading Control (AB197345)

10%SDS-PAGE

All lanes:

Western blot - Anti-Actin antibody - Loading Control (ab197345) at 1/500 dilution

Lane 1:

Cinnamomum camphora leaves lysate at 50 µg

Lane 2:

Nicotiana tabacum leaves lysate at 50 µg

Lane 3:

Triticum aestivuml leaves lysate at 50 µg

Lane 4:

Arabidopsis thaliana leaves lysate at 50 µg

Lane 5:

Brassica campestris flowers leaves lysate at 50 µg

Lane 6:

Brassica campestris leaves lysate at 50 µg

Lane 7:

Vicia Faba leaves lysate at 50 µg

Secondary

All lanes:

Anti-rabbit IgG at 1/8000 dilution

Predicted band size: 42 kDa

true

Exposure time: 20s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Arabidopsis thaliana, Cinnamomum camphora, Tobacco, Wheat, Brassica rapa, Fava Bean

Applications

applications

Immunogen

Synthetic Peptide within Arabidopsis thaliana ACT12. The exact immunogen used to generate this antibody is proprietary information.

P53497

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Arabidopsis thaliana": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "Brassica rapa": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "Cinnamomum camphora": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "Fava Bean": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "Tobacco": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "Wheat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Actin also known as globular (G-actin) or filamentous (F-actin) actin plays a central role in cell structure and movement. This protein has a molecular weight of approximately 42 kDa and resides abundantly in eukaryotic cells especially in muscle and cytoskeletal structures. It comes in several isoforms with varying expression profiles across tissues. Actin can undergo polymerization from its G-actin monomers into F-actin filaments a process that is reversible through depolymerization allowing for dynamic cellular functions.

Biological function summary

Actin contributes to various cellular processes by forming the cytoskeleton which provides mechanical support and determines cell shape. Actin also facilitates cell motility division and intracellular transport through rapid polymerization and depolymerization cycles. Within cells actin associates with other proteins to form complexes such as the Arp2/3 complex which assists actin in the branching of filaments critical for pushing forward the cell's leading edge during movement. Techniques like actin immunofluorescence help visualize actin filaments within cells revealing its extensive network.

Pathways

Actin plays an integral role in pathways like cell movement and signal transduction. The Rho family of GTPases regulates actin cytoskeleton rearrangements influencing cell shape and migration. Actin function interactions involve proteins like myosin forming actomyosin complexes essential for muscle contraction and other cell motility activities. Actin polymerization and depolymerization cycles are key to the dynamic regulation within these pathways ensuring adequate cellular responses to environmental signals.

Mutations or misregulation of actin and its associated pathways link to conditions such as cardiomyopathies and cancer metastasis. In familial cardiomyopathy actin mutations disrupt normal cardiac muscle contraction. In cancer altered actin polymerization and depolymerization enable invasive cell migration facilitating metastasis. Proteins like myosin and tropomyosin associate with actin in these pathologies illustrating the impact of actin dynamics on disease progression and highlighting actin as a therapeutic target. Anti-actin antibodies can be used in research and diagnostics to better understand these disorders.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Essential component of cell cytoskeleton; plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. This is considered as one of the reproductive actins.

See full target information ACT12

style

left-column

Publications (30)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8750 PubMed41034216

2025

HY5 integrates light and electrical signaling to trigger a jasmonate burst for nematode defense in tomato.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Sun,Chaoyi Hu,Changan Zhu,Jiaxing Cai,Guoting Wang,Xiaojian Xia,Kai Shi,Yanhong Zhou,Jingquan Yu,Christine H Foyer,Jie Zhou

PLoS genetics 21:e1011758 PubMed40788958

2025

Threshold-dependent negative autoregulation of PIF4 gene expression optimizes growth and fitness in Arabidopsis.

Applications

Unspecified application

Species

Unspecified reactive species

Sreya Das,Vikas Garhwal,Krishanu Mondal,Dipjyoti Das,Sreeramaiah N Gangappa

The Plant cell 37: PubMed40729411

2025

Glutathionylation-mediated degradation of a cap-binding protein enhances Arabidopsis resistance to Plutella xylostella.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Lin,Hui Ye,Mengjie Zhao,Xingzhi Chen,Jing Ma,Chuanhong Wang,Tengyue Wang,Zhen Tao,Yibing Zhao,Qingyang Zhang,Jun Lai,Xinqiao Zhang,Jinghui Dong,Peijin Li

Plant direct 9:e700042 PubMed39950159

2025

Unequal Genetic Redundancies Among MYC bHLH Transcription Factors Underlie Seedling Photomorphogenesis in Arabidopsis.

Applications

Unspecified application

Species

Unspecified reactive species

Vikas Garhwal,Sreya Das,Sreeramaiah N Gangappa

Nature communications 16:433 PubMed39762263

2025

Cooperative condensation of RNA-DIRECTED DNA METHYLATION 16 splicing isoforms enhances heat tolerance in Arabidopsis.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Ma,Shuai Li,Tengyue Wang,Zhen Tao,Shijie Huang,Ning Lin,Yibing Zhao,Chuanhong Wang,Peijin Li

The Plant cell 37: PubMed39667074

2024

SnRK1α1-mediated RBOH1 phosphorylation regulates reactive oxygen species to enhance tolerance to low nitrogen in tomato.

Applications

Unspecified application

Species

Unspecified reactive species

Xuelian Zheng,Hongfei Yang,Jinping Zou,Weiduo Jin,Zhenyu Qi,Ping Yang,Jingquan Yu,Jie Zhou

Journal of cellular and molecular medicine 28:e70159 PubMed39495769

2024

Inhibiting YAP1 reduced abdominal aortic aneurysm formation by suppressing adventitial fibroblast phenotype transformation and migration.

Applications

Unspecified application

Species

Unspecified reactive species

Cuiping Xie,Yanting Hu,Zhehui Yin

The New phytologist 244:1901-1915 PubMed39233529

2024

An activity-based sensing fluorogenic probe for monitoring O-methyltransferase in plants.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Lei Jia,Lixiang Zhu,Yuanyu Li,Pan Zhang,Xiao Chen,Kai Shao,Jingxian Feng,Shi Qiu,Jiaran Geng,Yingbo Yang,Zongtai Wu,Jingshi Xue,Ping Wang,Wansheng Chen,Ying Xiao

The New phytologist 245:1106-1123 PubMed39155750

2024

Strigolactones positively regulate HY5-dependent autophagy and the degradation of ubiquitinated proteins in response to cold stress in tomato.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Chi,Xinlin Chen,Changan Zhu,Jiajian Cao,Hui Li,Ying Fu,Guochen Qin,Jun Zhao,Jingquan Yu,Jie Zhou

Molecular therapy. Methods & clinical development 31:101145 PubMed38027065

2023

AAV2-antiVEGFscFv gene therapy for retinal neovascularization.

Applications

Unspecified application

Species

Unspecified reactive species

Ni Han,Xin Xu,Ying Liu,Guangzuo Luo

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Actin antibody - Loading Control

Western blot - Anti-Actin antibody - Loading Control (AB197345)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (30)

Product promise