AB209857

Anti-Actin antibody - Loading Control

Lab Essentials
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(6 Publications)

Rabbit Polyclonal alpha skeletal muscle Actin antibody. Suitable for IHC, WB, IHC-P, IHC - Wmt and reacts with Zebrafish samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ACTA1 aa 1-200.

View Alternative Names

ACTA, ACTA1, Alpha-actin-1

4 Images

Supplier Data

Immunohistochemistry - Anti-Actin antibody - Loading Control (AB209857)

Immunohistochemical analysis of agarose-embedded Zebrafish embryo tail section labeling Actin with ab209857 at 1/200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody - Loading Control (AB209857)

Immunohistochemical analysis of paraffin-embedded zebrafish tissue labeling Actin with ab209857 at 1/300 dilution.

IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-Actin antibody - Loading Control (AB209857)

Whole mount immunohistochemical analysis of of 1 day-post-fertilization zebrafish embryo labeling Actin with ab209857 at 1/50 dilution.

Supplier Data

Western blot - Anti-Actin antibody - Loading Control (AB209857)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Actin antibody - Loading Control (ab209857) at 1/1000 dilution

All lanes:

Zebrafish heart tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 42 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Zebrafish

Applications

WB, IHC, IHC - Wmt, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ACTA1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P68133

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCWmt" : {"fullname" : "IHC - Wholemount", "shortname":"IHC - Wmt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Zebrafish": { "IHC-species-checked": "testedAndGuaranteed", "IHC-species-dilution-info": "1/100 - 1/1000", "IHC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Suggested antigen retrieval using 1mM EDTA-NaOH (pH8.0) 95C,1Hr", "IHCWmt-species-checked": "testedAndGuaranteed", "IHCWmt-species-dilution-info": "1/50 - 1/500", "IHCWmt-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Actin also known as globular (G-actin) or filamentous (F-actin) actin plays a central role in cell structure and movement. This protein has a molecular weight of approximately 42 kDa and resides abundantly in eukaryotic cells especially in muscle and cytoskeletal structures. It comes in several isoforms with varying expression profiles across tissues. Actin can undergo polymerization from its G-actin monomers into F-actin filaments a process that is reversible through depolymerization allowing for dynamic cellular functions.

Biological function summary

Actin contributes to various cellular processes by forming the cytoskeleton which provides mechanical support and determines cell shape. Actin also facilitates cell motility division and intracellular transport through rapid polymerization and depolymerization cycles. Within cells actin associates with other proteins to form complexes such as the Arp2/3 complex which assists actin in the branching of filaments critical for pushing forward the cell's leading edge during movement. Techniques like actin immunofluorescence help visualize actin filaments within cells revealing its extensive network.

Pathways

Actin plays an integral role in pathways like cell movement and signal transduction. The Rho family of GTPases regulates actin cytoskeleton rearrangements influencing cell shape and migration. Actin function interactions involve proteins like myosin forming actomyosin complexes essential for muscle contraction and other cell motility activities. Actin polymerization and depolymerization cycles are key to the dynamic regulation within these pathways ensuring adequate cellular responses to environmental signals.

Mutations or misregulation of actin and its associated pathways link to conditions such as cardiomyopathies and cancer metastasis. In familial cardiomyopathy actin mutations disrupt normal cardiac muscle contraction. In cancer altered actin polymerization and depolymerization enable invasive cell migration facilitating metastasis. Proteins like myosin and tropomyosin associate with actin in these pathologies illustrating the impact of actin dynamics on disease progression and highlighting actin as a therapeutic target. Anti-actin antibodies can be used in research and diagnostics to better understand these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

See full target information ACTA1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 29:12 PubMed39534704

2024

Culture supernatant of tachyzoites inhibits the proliferation and metastasis of bladder cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shutong Du,Yiwei Yang,Tianfei Wu,Li Zhang,Hailong Li,Yan Lv

International journal of experimental pathology 105:21-32 PubMed38054576

2023

Circular RNA circVAPA modulates macrophage pyroptosis in sepsis-induced acute lung injury through targeting miR-212-3p/Sirt1/Nrf2/NLRP3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yanjing Huang,Jinquan Lin,Zhiwei Wu,Yiming Li

Stem cells international 2022:5609501 PubMed36277041

2022

MFG-E8 Exerts Neuroprotection in Neural Stem Cells Induced by Anesthetic Sevoflurane via Regulating the PI3K/AKT Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Minmin Cai,Liufang Sheng

Journal of lipid research 63:100199 PubMed35315333

2022

Consequences of excessive glucosylsphingosine in glucocerebrosidase-deficient zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Lindsey T Lelieveld,Sophie Gerhardt,Saskia Maas,Kimberley C Zwiers,Claire de Wit,Ernst H Beijk,Maria J Ferraz,Marta Artola,Annemarie H Meijer,Christian Tudorache,Daniela Salvatori,Rolf G Boot,Johannes M F G Aerts

Toxicology research 11:77-87 PubMed35237413

2022

JC-10 probe as a novel method for analyzing the mitochondrial membrane potential and cell stress in whole zebrafish embryos.

Applications

Unspecified application

Species

Unspecified reactive species

Nadin Younes,Bana S Alsahan,Asmaa J Al-Mesaifri,Sahar I Da'as,Gianfranco Pintus,Amin F Majdalawieh,Gheyath K Nasrallah

Human cell 32:306-315 PubMed31104300

2019

ADRB2 suppresses IL-13-induced allergic rhinitis inflammatory cytokine regulated by miR-15a-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Li Wang,Qiaoying Lv,Xicheng Song,Kun Jiang,Junhong Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Actin antibody - Loading Control

Immunohistochemistry - Anti-Actin antibody - Loading Control (AB209857)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody - Loading Control (AB209857)

IHC - Wholemount - Anti-Actin antibody - Loading Control (AB209857)

Western blot - Anti-Actin antibody - Loading Control (AB209857)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise