Mouse Monoclonal alpha skeletal muscle Actin antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat, Drosophila melanogaster samples. Cited in 3 publications.
Images
![Western blot - Anti-Actin antibody [mAbGEa] - Loading Control (AB230169), expandable thumbnail](https://content.abcam.com/products/images/actin-antibody-mabgea-loading-control-ab230169--western-blot-img6560.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Actin antibody [mAbGEa] - Loading Control (AB230169)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody [mAbGEa] (AB230169), expandable thumbnail](https://content.abcam.com/products/images/actin-antibody-mabgea-loading-control-ab230169--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img103356.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody [mAbGEa] (AB230169)")
Publications
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- Journal of experimental botany 71:435-4492019Expression of galactinol synthase from Ammopiptanthus nanus in tomato improves tolerance to cold stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuDong Liu,Li Zhang,SiDa Meng,YuFeng Liu,XiaOmeng Zhao,ChunPeng Pang,HuiDong Zhang,Tao Xu,Yi He,MingFang Qi,Tianlai LiPubMed 31616940
- The Plant cell 24:2041-572012Plant vegetative and animal cytoplasmic actins share functional competence for spatial development with protists.
- The Plant journal : for cell and molecular biology 18:681-911999The late pollen-specific actins in angiosperms.
Key facts
- Isotype
- IgM
- Host species
- Mouse
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Expected | Tested |
| Drosophila melanogaster | Expected | Tested |
| Fungi | Predicted | Predicted |
| Mammals | Predicted | Predicted |
| Plants | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Drosophila melanogaster | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Xenopus laevis, Plants, Mammals, Fungi | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes Abcam recommends a 3% milk block for this product. |
Species Rat | Dilution info 1 µg/mL | Notes Abcam recommends a 3% milk block for this product. |
Species Drosophila melanogaster | Dilution info 1 µg/mL | Notes Abcam recommends a 3% milk block for this product. |
Species Human | Dilution info 1 µg/mL | Notes Abcam recommends a 3% milk block for this product. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Xenopus laevis, Plants, Mammals, Fungi | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Version
7 products for Alternative Product
Target data
Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Alternative names
ACTA, ACTA1, Alpha-actin-1
Recommended products
Mouse Monoclonal alpha skeletal muscle Actin antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat, Drosophila melanogaster samples. Cited in 3 publications.
Key facts
- Isotype
- IgM
- Host species
- Mouse
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- mAbGEa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Actin also known as globular (G-actin) or filamentous (F-actin) actin plays a central role in cell structure and movement. This protein has a molecular weight of approximately 42 kDa and resides abundantly in eukaryotic cells especially in muscle and cytoskeletal structures. It comes in several isoforms with varying expression profiles across tissues. Actin can undergo polymerization from its G-actin monomers into F-actin filaments a process that is reversible through depolymerization allowing for dynamic cellular functions.
- Biological function summary
Actin contributes to various cellular processes by forming the cytoskeleton which provides mechanical support and determines cell shape. Actin also facilitates cell motility division and intracellular transport through rapid polymerization and depolymerization cycles. Within cells actin associates with other proteins to form complexes such as the Arp2/3 complex which assists actin in the branching of filaments critical for pushing forward the cell's leading edge during movement. Techniques like actin immunofluorescence help visualize actin filaments within cells revealing its extensive network.
- Pathways
Actin plays an integral role in pathways like cell movement and signal transduction. The Rho family of GTPases regulates actin cytoskeleton rearrangements influencing cell shape and migration. Actin function interactions involve proteins like myosin forming actomyosin complexes essential for muscle contraction and other cell motility activities. Actin polymerization and depolymerization cycles are key to the dynamic regulation within these pathways ensuring adequate cellular responses to environmental signals.
- Associated diseases and disorders
Mutations or misregulation of actin and its associated pathways link to conditions such as cardiomyopathies and cancer metastasis. In familial cardiomyopathy actin mutations disrupt normal cardiac muscle contraction. In cancer altered actin polymerization and depolymerization enable invasive cell migration facilitating metastasis. Proteins like myosin and tropomyosin associate with actin in these pathologies illustrating the impact of actin dynamics on disease progression and highlighting actin as a therapeutic target. Anti-actin antibodies can be used in research and diagnostics to better understand these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-Actin antibody [mAbGEa] - Loading Control (ab230169), expandable thumbnail](https://content.abcam.com/products/images/actin-antibody-mabgea-loading-control-ab230169--western-blot-img6560.jpg "Western blot - Anti-Actin antibody [mAbGEa] - Loading Control (ab230169)")
Western blot - Anti-Actin antibody [mAbGEa] - Loading Control (ab230169)
Actin Western blot staining using mouse Anti-Actin antibody
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab230169 overnight at 4°C. Antibody binding was detected using a goat anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - Anti-Actin antibody [mAbGEa] - Loading Control (ab230169) at 1 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 2: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 20 µg
Lane 3: PC12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 20 µg
Lane 4: Drosophila lysate at 20 µg
SecondaryAll lanes: HRP conjugated Goat Anti-Mouse IgG (H+L) at 1/1500 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Exposure time: 10s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody [mAbGEa] (ab230169), expandable thumbnail](https://content.abcam.com/products/images/actin-antibody-mabgea-loading-control-ab230169--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img103356.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody [mAbGEa] (ab230169)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody [mAbGEa] (ab230169)
IHC image of beta actin staining in a section of formalin-fixed paraffin-embedded normal human normal colon* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab230169, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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