AB230169

Anti-Actin antibody [mAbGEa] - Loading Control

BOND RX™ Validated
Lab Essentials
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(9 Publications)

Mouse Monoclonal alpha skeletal muscle Actin antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat, Drosophila melanogaster samples. Cited in 9 publications.

View Alternative Names

ACTA, ACTA1, Alpha-actin-1

2 Images

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody [mAbGEa] - Loading Control (AB230169)

IHC image of beta actin staining in a section of formalin-fixed paraffin-embedded normal human normal colon* performed on a Leica BOND^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab230169, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Lab

Western blot - Anti-Actin antibody [mAbGEa] - Loading Control (AB230169)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab230169 overnight at 4°C. Antibody binding was detected using a goat anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ab133406.

All lanes:

Western blot - Anti-Actin antibody [mAbGEa] - Loading Control (ab230169) at 1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 20 µg

Lane 3:

PC12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 20 µg

Lane 4:

Drosophila lysate at 20 µg

Secondary

All lanes:

HRP conjugated Goat Anti-Mouse IgG (H+L) at 1/1500 dilution

Predicted band size: 42 kDa

true

Exposure time: 10s

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

mAbGEa

Isotype

IgM

Carrier free

Reacts with

Mouse, Rat, Human, Drosophila melanogaster

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "Abcam recommends a 3% milk block for this product." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "Abcam recommends a 3% milk block for this product." }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "Abcam recommends a 3% milk block for this product." }, "Drosophila melanogaster": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "Abcam recommends a 3% milk block for this product." }, "Fungi": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mammals": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Plants": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Actin also known as globular (G-actin) or filamentous (F-actin) actin plays a central role in cell structure and movement. This protein has a molecular weight of approximately 42 kDa and resides abundantly in eukaryotic cells especially in muscle and cytoskeletal structures. It comes in several isoforms with varying expression profiles across tissues. Actin can undergo polymerization from its G-actin monomers into F-actin filaments a process that is reversible through depolymerization allowing for dynamic cellular functions.

Biological function summary

Actin contributes to various cellular processes by forming the cytoskeleton which provides mechanical support and determines cell shape. Actin also facilitates cell motility division and intracellular transport through rapid polymerization and depolymerization cycles. Within cells actin associates with other proteins to form complexes such as the Arp2/3 complex which assists actin in the branching of filaments critical for pushing forward the cell's leading edge during movement. Techniques like actin immunofluorescence help visualize actin filaments within cells revealing its extensive network.

Pathways

Actin plays an integral role in pathways like cell movement and signal transduction. The Rho family of GTPases regulates actin cytoskeleton rearrangements influencing cell shape and migration. Actin function interactions involve proteins like myosin forming actomyosin complexes essential for muscle contraction and other cell motility activities. Actin polymerization and depolymerization cycles are key to the dynamic regulation within these pathways ensuring adequate cellular responses to environmental signals.

Mutations or misregulation of actin and its associated pathways link to conditions such as cardiomyopathies and cancer metastasis. In familial cardiomyopathy actin mutations disrupt normal cardiac muscle contraction. In cancer altered actin polymerization and depolymerization enable invasive cell migration facilitating metastasis. Proteins like myosin and tropomyosin associate with actin in these pathologies illustrating the impact of actin dynamics on disease progression and highlighting actin as a therapeutic target. Anti-actin antibodies can be used in research and diagnostics to better understand these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

See full target information ACTA1

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eads3718 PubMed39908379

2025

Chemical genetics reveals cross-regulation of plant developmental signaling by the immune peptide-receptor pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Arvid Herrmann,Krishna Mohan Sepuru,Pengfei Bai,Hitoshi Endo,Ayami Nakagawa,Shuhei Kusano,Asraa Ziadi,Hiroe Kato,Ayato Sato,Jun Liu,Libo Shan,Seisuke Kimura,Kenichiro Itami,Naoyuki Uchida,Shinya Hagihara,Keiko U Torii

Theranostics 14:7569-7588 PubMed39659565

2024

FBLN7 mediates vascular smooth muscle cell phenotype switching and vascular remodeling in hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Guoqing Yao,Xuehui Zheng,Yang Hu,Yuan Zhao,Binghui Kong,Yun Ti,Pei Li Bu

Nature communications 15:4358 PubMed38778058

2024

DNA methylation-based high-resolution mapping of long-distance chromosomal interactions in nucleosome-depleted regions.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Li,James Lee,Lu Bai

Heliyon 10:e24499 PubMed38298727

2024

CPY27B1-related vitamin D deficiency contributes to the impaired function of CD4T cells in Recurrent spontaneous miscarriage.

Applications

Unspecified application

Species

Unspecified reactive species

Chaoyan Yue,Yanhui Ma,Mingyan Wang,Minmin Yuan,Yi Meng,Zhiheng Wang,Chunmei Ying

Biomedicines 11: PubMed37892970

2023

Enhancing Muscle Intracellular Ca Homeostasis and Glucose Uptake: Passive Pulsatile Shear Stress Treatment in Type 2 Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Arkady Uryash,Jordan Umlas,Alfredo Mijares,Jose A Adams,Jose R Lopez

Nature plants 9:112-127 PubMed36539597

2022

Direct attenuation of Arabidopsis ERECTA signalling by a pair of U-box E3 ligases.

Applications

Unspecified application

Species

Unspecified reactive species

Liangliang Chen,Alicia M Cochran,Jessica M Waite,Ken Shirasu,Shannon M Bemis,Keiko U Torii

Journal of experimental botany 71:435-449 PubMed31616940

2019

Expression of galactinol synthase from Ammopiptanthus nanus in tomato improves tolerance to cold stress.

Applications

Unspecified application

Species

Unspecified reactive species

YuDong Liu,Li Zhang,SiDa Meng,YuFeng Liu,XiaOmeng Zhao,ChunPeng Pang,HuiDong Zhang,Tao Xu,Yi He,MingFang Qi,Tianlai Li

The Plant cell 24:2041-57 PubMed22589468

2012

Plant vegetative and animal cytoplasmic actins share functional competence for spatial development with protists.

Applications

Species

Owl

Muthugapatti K Kandasamy,Elizabeth C McKinney,Eileen Roy,Richard B Meagher

The Plant journal : for cell and molecular biology 18:681-91 PubMed10417720

1999

The late pollen-specific actins in angiosperms.

Applications

Species

Owl

M K Kandasamy,E C McKinney,R B Meagher

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Actin antibody [mAbGEa] - Loading Control

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody [mAbGEa] - Loading Control (AB230169)

Western blot - Anti-Actin antibody [mAbGEa] - Loading Control (AB230169)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reagents, Controls & Accessories

Reagents, Controls & Accessories

Secondary Antibodies

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Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Product promise