Skip to main content

Mouse Monoclonal alpha skeletal muscle Actin antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat, Drosophila melanogaster samples. Cited in 3 publications.

Be the first to review this product! Submit a review

Images

Western blot - Anti-Actin antibody [mAbGEa] - Loading Control (AB230169), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody [mAbGEa] (AB230169), expandable thumbnail

Publications

Key facts

Isotype
IgM
Host species
Mouse
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested
Mouse
Expected
Tested
Rat
Expected
Tested
Drosophila melanogaster
Expected
Tested
Fungi
Predicted
Predicted
Mammals
Predicted
Predicted
Plants
Predicted
Predicted
Xenopus laevis
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat, Drosophila melanogaster
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Xenopus laevis, Plants, Mammals, Fungi
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1 µg/mL
Notes

Abcam recommends a 3% milk block for this product.

Species
Rat
Dilution info
1 µg/mL
Notes

Abcam recommends a 3% milk block for this product.

Species
Drosophila melanogaster
Dilution info
1 µg/mL
Notes

Abcam recommends a 3% milk block for this product.

Species
Human
Dilution info
1 µg/mL
Notes

Abcam recommends a 3% milk block for this product.

Predicted
Predicted

Species
Xenopus laevis, Plants, Mammals, Fungi
Dilution info
-
Notes

-

Associated Products

Select an associated product type

5 products for Alternative Version

7 products for Alternative Product

Target data

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Alternative names

Recommended products

Mouse Monoclonal alpha skeletal muscle Actin antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat, Drosophila melanogaster samples. Cited in 3 publications.

Key facts

Isotype
IgM
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
mAbGEa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Actin also known as globular (G-actin) or filamentous (F-actin) actin plays a central role in cell structure and movement. This protein has a molecular weight of approximately 42 kDa and resides abundantly in eukaryotic cells especially in muscle and cytoskeletal structures. It comes in several isoforms with varying expression profiles across tissues. Actin can undergo polymerization from its G-actin monomers into F-actin filaments a process that is reversible through depolymerization allowing for dynamic cellular functions.

Biological function summary

Actin contributes to various cellular processes by forming the cytoskeleton which provides mechanical support and determines cell shape. Actin also facilitates cell motility division and intracellular transport through rapid polymerization and depolymerization cycles. Within cells actin associates with other proteins to form complexes such as the Arp2/3 complex which assists actin in the branching of filaments critical for pushing forward the cell's leading edge during movement. Techniques like actin immunofluorescence help visualize actin filaments within cells revealing its extensive network.

Pathways

Actin plays an integral role in pathways like cell movement and signal transduction. The Rho family of GTPases regulates actin cytoskeleton rearrangements influencing cell shape and migration. Actin function interactions involve proteins like myosin forming actomyosin complexes essential for muscle contraction and other cell motility activities. Actin polymerization and depolymerization cycles are key to the dynamic regulation within these pathways ensuring adequate cellular responses to environmental signals.

Associated diseases and disorders

Mutations or misregulation of actin and its associated pathways link to conditions such as cardiomyopathies and cancer metastasis. In familial cardiomyopathy actin mutations disrupt normal cardiac muscle contraction. In cancer altered actin polymerization and depolymerization enable invasive cell migration facilitating metastasis. Proteins like myosin and tropomyosin associate with actin in these pathologies illustrating the impact of actin dynamics on disease progression and highlighting actin as a therapeutic target. Anti-actin antibodies can be used in research and diagnostics to better understand these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-Actin antibody [mAbGEa] - Loading Control (ab230169), expandable thumbnail

    Western blot - Anti-Actin antibody [mAbGEa] - Loading Control (ab230169)

    Actin Western blot staining using mouse Anti-Actin antibody

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab230169 overnight at 4°C. Antibody binding was detected using a goat anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

    All lanes: Western blot - Anti-Actin antibody [mAbGEa] - Loading Control (ab230169) at 1 µg/mL

    Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 2: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 20 µg

    Lane 3: PC12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 20 µg

    Lane 4: Drosophila lysate at 20 µg

    Secondary

    All lanes: HRP conjugated Goat Anti-Mouse IgG (H+L) at 1/1500 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42 kDa

    Exposure time: 10s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody [mAbGEa] (ab230169), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody [mAbGEa] (ab230169)

    IHC image of beta actin staining in a section of formalin-fixed paraffin-embedded normal human normal colon* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab230169, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com