Skip to main content

Rat Monoclonal alpha skeletal muscle Actin antibody. Suitable for IHC-P and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Tissue preparation containing Actin protein.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody [MAC 237] (AB50591), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody [MAC 237] - Cytoskeleton marker (AB50591), expandable thumbnail

Publications

Key facts

Isotype
IgG2b
Host species
Rat
Storage buffer

Preservative: 0.1% Sodium azide
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Tissue preparation containing Actin protein. The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Human
Tested

Tested
Tested

Species
Human
Dilution info
5 µg/mL
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Alternative names

ACTA, ACTA1, Alpha-actin-1

Recommended products

Rat Monoclonal alpha skeletal muscle Actin antibody. Suitable for IHC-P and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Tissue preparation containing Actin protein.

Key facts

Isotype
IgG2b
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Tissue preparation containing Actin protein. The exact immunogen used to generate this antibody is proprietary information.
Clone number
MAC 237
Purification technique
Affinity purification Protein A
Specificity

Antibody reacts with actin in Lethocerus and Drosophila flight and non-flight muscle and with actin in flight muscles of all other insect species tested. Also reacts with mammalian actin (tested vs. rat). Antibody reacts with arthrin in flight muscles of Hemiptera and Diptera.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Actin also known as globular (G-actin) or filamentous (F-actin) actin plays a central role in cell structure and movement. This protein has a molecular weight of approximately 42 kDa and resides abundantly in eukaryotic cells especially in muscle and cytoskeletal structures. It comes in several isoforms with varying expression profiles across tissues. Actin can undergo polymerization from its G-actin monomers into F-actin filaments a process that is reversible through depolymerization allowing for dynamic cellular functions.

Biological function summary

Actin contributes to various cellular processes by forming the cytoskeleton which provides mechanical support and determines cell shape. Actin also facilitates cell motility division and intracellular transport through rapid polymerization and depolymerization cycles. Within cells actin associates with other proteins to form complexes such as the Arp2/3 complex which assists actin in the branching of filaments critical for pushing forward the cell's leading edge during movement. Techniques like actin immunofluorescence help visualize actin filaments within cells revealing its extensive network.

Pathways

Actin plays an integral role in pathways like cell movement and signal transduction. The Rho family of GTPases regulates actin cytoskeleton rearrangements influencing cell shape and migration. Actin function interactions involve proteins like myosin forming actomyosin complexes essential for muscle contraction and other cell motility activities. Actin polymerization and depolymerization cycles are key to the dynamic regulation within these pathways ensuring adequate cellular responses to environmental signals.

Associated diseases and disorders

Mutations or misregulation of actin and its associated pathways link to conditions such as cardiomyopathies and cancer metastasis. In familial cardiomyopathy actin mutations disrupt normal cardiac muscle contraction. In cancer altered actin polymerization and depolymerization enable invasive cell migration facilitating metastasis. Proteins like myosin and tropomyosin associate with actin in these pathologies illustrating the impact of actin dynamics on disease progression and highlighting actin as a therapeutic target. Anti-actin antibodies can be used in research and diagnostics to better understand these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody [MAC 237] (ab50591), expandable thumbnail
    This image is courtesy of a customer review submitted by Anne Sailer

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody [MAC 237] (ab50591)

    ab50591 staining Actin in mouse proximal colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% NDS for 24 hours at 4°C; antigen retrieval was by heat mediation in buffer, pH9. Samples were incubated with primary antibody (1/100 in 5% BSA/NDS) for 24 hours at 4°C. An undiluted Alexa Fluor® 647-conjugated donkey anti-rat IgG polyclonal was used as the secondary antibody.

    Also stained with Anti-E Cadherin antibody [M168] - C-terminal ab76055 (green) at 1/100.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody [MAC 237] - Cytoskeleton marker (ab50591), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody [MAC 237] - Cytoskeleton marker (ab50591)

    Actin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rat Anti-Actin antibody

    IHC image of Actin staining in Human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab50591, 5μg/ml, for 15 mins at room temperature. A Goat anti-Rat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com