AB155688

Anti-Actin Regulatory Protein CAPG/MCP antibody

Name: Anti-Actin Regulatory Protein CAPG/MCP antibody (ab155688) | Abcam
Brand: Abcam Limited
SKU: AB155688
Price: 545 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(9 Publications)

Rabbit Polyclonal Actin Regulatory Protein CAPG/MCP antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CAPG aa 150-350.

View Alternative Names

AFCP, MCP, CAPG, Macrophage-capping protein, Actin regulatory protein CAP-G

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin Regulatory Protein CAPG/MCP antibody (AB155688)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin Regulatory Protein CAPG/MCP antibody (AB155688)

Immunohistochemical analysis of paraffin-embedded Human NCIN87 xenograft tissue labeling Actin Regulatory Protein CAPG/MCP with ab155688 at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Actin Regulatory Protein CAPG/MCP antibody (AB155688)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Actin Regulatory Protein CAPG/MCP antibody (AB155688)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling CAPG protein at cytoplasm and nucleus using ab155688 at a 1/400 dilution (Green). Hoechst 33342 was used for nuclear staining (Blue). Scale bar = 10 µm

Unknown

Western blot - Anti-Actin Regulatory Protein CAPG/MCP antibody (AB155688)

10% SDS PAGE

All lanes:

Western blot - Anti-Actin Regulatory Protein CAPG/MCP antibody (ab155688) at 1/2000 dilution

All lanes:

NIH 3T3 whole cell lysate at 30 µg

Predicted band size: 38 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human CAPG aa 150-350. The exact immunogen used to generate this antibody is proprietary information.

P40121

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Actin Regulatory Protein CAPG also known as MCP is an important cytoskeletal protein involved in actin filament dynamics. Other common names include CAPG protein and capping proteins. CAPG binds to the barbed ends of actin filaments modulating their length and polymerization. This protein has a molecular weight of approximately 39 kDa. It is expressed in various tissues with significant expression in HeLa cells which are often used in studies related to actin regulation.

Biological function summary

This actin regulatory protein serves as an important player in the remodeling of the actin cytoskeleton. CAPG functions by capping the fast-growing ends of actin filaments which influences cell motility and structure. It is not known to be part of a larger protein complex but interacts closely with other actin-modulating proteins. MCP's regulation of cytoskeletal dynamics allows cells to respond swiftly to environmental changes assisting cellular processes like migration and secretion.

Pathways

CAPG is intricately involved in pathways controlling cell motility and structure including the cytoskeletal rearrangement pathway. It works alongside proteins such as cofilin and Arp2/3 complex which also regulate actin dynamics. These proteins together facilitate processes like cell division and migration making their interactions essential for proper cellular function.

CAPG's regulation is pertinent in cancer metastasis particularly in its relation to HeLa cells used in cancer studies. It affects tumor cell invasiveness by altering the cytoskeleton connecting it to processes involving other regulatory proteins like Rac1. Additionally CAPG plays a role in neurodegenerative disorders by impacting neuronal cell motility where interactions with actin-modulating proteins are important. Understanding CAPG's role gives insight into potential interventions for these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA.

See full target information CAPG

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 21:5476-5495 PubMed40959275

2025

Increased CAPG inhibits ferroptosis to drive tumor proliferation and sorafenib resistance in hepatocellular carcinoma via the WDR74-p53-SLC7A11 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Quan,Fan Yao,Wenfeng Liu,Bei Tang,Miao Li,Shenxin Lu,Jinghuan Li,Rongxin Chen,Zhenggang Ren,Xin Yin

International ophthalmology 44:437 PubMed39578251

2024

Carbonic anhydrase inhibitor alleviates retinal barrier toxicity in paclitaxel-induced retinopathy and macular edema by inhibiting CAXIV.

Applications

Unspecified application

Species

Unspecified reactive species

Ya-Ting Ye,Ya-Li Niu,Zi-Yi Zhou,Yu Sun,Tian-Fang Chang,Yu-Tong Jing,Qian Bai,Zhao-Jie Chu

Cell death discovery 10:15 PubMed38191512

2024

CAPG is a novel biomarker for early gastric cancer and is involved in the Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Long,JiaQi Wu,Yu Shen,Chenxiao Gan,Chuandong Zhang,Gang Wang,Jiyong Jing,Chenjing Zhang,Wensheng Pan

World journal of surgical oncology 20:347 PubMed36258216

2022

CapG promoted nasopharyngeal carcinoma cell motility involving Rho motility pathway independent of ROCK.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Fu,Xiuzhi Zhang,Xujun Liang,Yongheng Chen,Zhuchu Chen,Zhefeng Xiao

Genes & diseases 10:239-253 PubMed37013030

2021

aggravates atherosclerosis by enhancing intestinal permeability and endothelial TLR4/NF-κB pathway in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Zhang,Xian Qin,Juhui Qiu,Tong Sun,Kai Qu,Ahmad Ud Din,Wenhua Yan,Tianhan Li,Yidan Chen,Wei Gu,Xiancai Rao,Guixue Wang

Oncology letters 18:1344-1350 PubMed31423196

2019

Analysis of clinical characteristics of macrophage capping protein (CAPG) gene expressed in glioma based on TCGA data and clinical experiments.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Fu,Mahati Shaya,Shaoshan Li,Yalikun Kugeluke,Yisireyili Dilimulati,Bo Liu,Qingjiu Zhou

OncoTargets and therapy 12:3933-3943 PubMed31190890

2019

Downregulation of NSD3 (WHSC1L1) inhibits cell proliferation and migration via ERK1/2 deactivation and decreasing CAPG expression in colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lanjuan Yi,Lanjie Yi,Qing Liu,Chen Li

Redox biology 24:101211 PubMed31108460

2019

Ferroptosis is governed by differential regulation of transcription in liver cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Zhang,Lutao Du,Yongxia Qiao,Xiaobai Zhang,Weisheng Zheng,Qi Wu,Yan Chen,Guoqing Zhu,Ya Liu,Zhixuan Bian,Susu Guo,Yueyue Yang,Lifang Ma,Yongchun Yu,Qiuhui Pan,Fenyong Sun,Jiayi Wang

Molecular and cellular endocrinology 404:91-101 PubMed25637714

2015

Estrogen and androgen regulate actin-remodeling and endocytosis-related genes during rat spermiation.

Applications

ICC/IF

Species

Rat

Anita Kumar,Kushaan Dumasia,Reshma Gaonkar,Shobha Sonawane,Leena Kadam,N H Balasinor

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Actin Regulatory Protein CAPG/MCP antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin Regulatory Protein CAPG/MCP antibody (AB155688)

Immunocytochemistry/ Immunofluorescence - Anti-Actin Regulatory Protein CAPG/MCP antibody (AB155688)

Western blot - Anti-Actin Regulatory Protein CAPG/MCP antibody (AB155688)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Product promise