AB181095

Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194-34]

Name: Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194-34] (ab181095) | Abcam
Brand: Abcam Limited
SKU: AB181095
Price: 340 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
KO Validated
What is this?

(1 Review)

(1 Publication)

Rabbit Recombinant Monoclonal Actin Regulatory Protein CAPG/MCP antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

AFCP, MCP, CAPG, Macrophage-capping protein, Actin regulatory protein CAP-G

3 Images

Lab

Western blot - Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194-34] (AB181095)

Lanes 1-4 : Merged signal (red and green). Green - ab181095 observed at 48 kDa. Red - loading control ab8245 observed at 36 kDa.

ab181095 Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194-34] was shown to specifically react with Actin Regulatory Protein CAPG/MCP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265766 (knockout cell lysate ab257378) was used. Wild-type and Actin Regulatory Protein CAPG/MCP knockout samples were subjected to SDS-PAGE. ab181095 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194-34] (ab181095) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

CAPG knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human CAPG (MCP) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-capg-mcp-knockout-hela-cell-line-ab265766'>ab265766</a>)

Lane 3:

THP-1 cell lysate at 20 µg

Lane 4:

U-937 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 38 kDa

Observed band size: 48 kDa

false

Supplier Data

Western blot - Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194-34] (AB181095)

All lanes:

Western blot - Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194-34] (ab181095) at 1/50000 dilution

All lanes:

U937 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 38 kDa,42 kDa

Observed band size: 42 kDa

false

Supplier Data

Western blot - Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194-34] (AB181095)

All lanes:

Western blot - Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194-34] (ab181095) at 1/10000 dilution

All lanes:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 38 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13194-34

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/50000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Actin Regulatory Protein CAPG also known as MCP is an important cytoskeletal protein involved in actin filament dynamics. Other common names include CAPG protein and capping proteins. CAPG binds to the barbed ends of actin filaments modulating their length and polymerization. This protein has a molecular weight of approximately 39 kDa. It is expressed in various tissues with significant expression in HeLa cells which are often used in studies related to actin regulation.

Biological function summary

This actin regulatory protein serves as an important player in the remodeling of the actin cytoskeleton. CAPG functions by capping the fast-growing ends of actin filaments which influences cell motility and structure. It is not known to be part of a larger protein complex but interacts closely with other actin-modulating proteins. MCP's regulation of cytoskeletal dynamics allows cells to respond swiftly to environmental changes assisting cellular processes like migration and secretion.

Pathways

CAPG is intricately involved in pathways controlling cell motility and structure including the cytoskeletal rearrangement pathway. It works alongside proteins such as cofilin and Arp2/3 complex which also regulate actin dynamics. These proteins together facilitate processes like cell division and migration making their interactions essential for proper cellular function.

CAPG's regulation is pertinent in cancer metastasis particularly in its relation to HeLa cells used in cancer studies. It affects tumor cell invasiveness by altering the cytoskeleton connecting it to processes involving other regulatory proteins like Rac1. Additionally CAPG plays a role in neurodegenerative disorders by impacting neuronal cell motility where interactions with actin-modulating proteins are important. Understanding CAPG's role gives insight into potential interventions for these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA.

See full target information CAPG

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 35:e21344 PubMed33566385

2021

Phosphatase of regenerating liver-3 regulates cancer cell metabolism in multiple myeloma.

Applications

Unspecified application

Species

Unspecified reactive species

Pegah Abdollahi,Esten N Vandsemb,Samah Elsaadi,Lisa M Røst,Rui Yang,Magnus A Hjort,Trygve Andreassen,Kristine Misund,Tobias S Slørdahl,Torstein B Rø,Anne-Marit Sponaas,Siver Moestue,Per Bruheim,Magne Børset

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194-34]

Western blot - Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194-34] (AB181095)

Western blot - Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194-34] (AB181095)

Western blot - Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194-34] (AB181095)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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