AB52301

Anti-activated Notch1 antibody

Name: Anti-activated Notch1 antibody (ab52301) | Abcam
Brand: Abcam Limited
SKU: AB52301
Price: 485 USD
Availability: InStock
Rating: 1 (2 reviews)

(2 Reviews)

(65 Publications)

Rabbit Polyclonal Notch1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 65 publications. Immunogen corresponding to Synthetic Peptide within Human NOTCH1 aa 1700-1800.

View Alternative Names

TAN1, NOTCH1, Neurogenic locus notch homolog protein 1, Notch 1, hN1, Translocation-associated notch protein TAN-1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-activated Notch1 antibody (AB52301)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-activated Notch1 antibody (AB52301)

Immunohistochemical analysis of paraffin-embedded human brain tissue sections labeling activated Notch1 with ab52301 at a dilution of 1/100. The picture on the right is treated with the synthesized peptide.

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Western blot - Anti-activated Notch1 antibody (AB52301)

All lanes:

Western blot - Anti-activated Notch1 antibody (ab52301) at 1/500 dilution

Lane 1:

NIH 3T3 cell extract treated with etoposide (25µM, 1 hour)

Lane 2:

NIH 3T3 cell extract treated with etoposide (25µM, 1 hour) with immunising peptide

Predicted band size: 273 kDa

Observed band size: 95 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human NOTCH1 aa 1700-1800. The exact immunogen used to generate this antibody is proprietary information.

P46531

Specificity

ab52301 detects endogenous levels of fragment of activated Notch 1 resulting from cleavage adjacent to Val1744.

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Reactivity data

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Product details

ab52301 is cleaved at Val 1744

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab52301 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage buffer

pH: 7 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Activated Notch1 also known as cleaved Notch1 and Notch 1 NICD is an intracellular domain resulting from Notch1 receptor cleavage. The molecular weight of Notch1 NICD varies but it is generally around 120-150 kDa depending on post-translational modifications. These receptors are present in many cell types including embryonic cells stem cells and various tissue-specific cells. They reside in the cell membrane and become activated upon interaction with specific ligands.

Biological function summary

Activated Notch1 is critical for regulating cell fate decisions including proliferation differentiation and apoptosis. It participates in a multi-protein complex that includes the CSL (CBF1/Su(H)/LAG-1) transcription factor modulating gene expression by binding to DNA. The role of activated Notch1 in maintaining the balance between cell growth and death is important in tissue homeostasis and development.

Pathways

Activated Notch1 plays a central role in the Notch signaling pathway an essential pathway for intercellular communication. This pathway involves several proteins including Delta and Jagged as ligands. It contributes to several critical developmental processes and is connected with the regulation of the NF-kB signaling pathway involved in immune responses and inflammation.

Activated Notch1 is significantly associated with T-cell acute lymphoblastic leukemia (T-ALL) and breast cancer. Abnormal expressions or mutations in Notch1 can lead to unchecked cellular proliferation in these conditions. Notch1 alterations often coexist with disruptions in other signaling proteins such as p53 contributing to disease pathology and presenting potential targets for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4(+) and CD8(+) cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved in mesoderm development, somite formation and neurogenesis. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Required for the THBS4 function in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury. Involved in determination of left/right symmetry by modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO).

See full target information NOTCH1

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Publications (65)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceuticals (Basel, Switzerland) 17: PubMed39598328

2024

Magnolol Inhibits High Fructose-Induced Podocyte Inflammation via Downregulation of TKFC/Sp1/HDAC4/Notch1 Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Ziang Zhou,Yumeng Wang,Yu Xing,Shuman Pan,Wanru Wang,Jie Yang,Wenyuan Wu,Jie Zhou,Luyi Huang,Qiongdan Liang,Dongmei Zhang,Lingdong Kong

Nature communications 15:10087 PubMed39572549

2024

DUSP6 regulates Notch1 signalling in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chin Wen Png,Madhushanee Weerasooriya,Heng Li,Xiaowen Hou,Fiona Yayuan Teo,Shiying Huang,Zheng Ser,Franklin Yau Kok Weng,Malini Rethnam,Gloryn Chia,Radoslaw M Sobota,Choon Seng Chong,Ker-Kan Tan,Yongliang Zhang

BMC complementary medicine and therapies 24:293 PubMed39090706

2024

The effect of salidroside in promoting endogenous neural regeneration after cerebral ischemia/reperfusion involves notch signaling pathway and neurotrophic factors.

Applications

Unspecified application

Species

Unspecified reactive species

Jiabing Zheng,Jizhou Zhang,Jing Han,Zhichang Zhao,Kan Lin

Experimental and therapeutic medicine 28:373 PubMed39091630

2024

Interference of FZD2 suppresses proliferation, vasculogenic mimicry and stemness in glioma cells via blocking the Notch/NF‑κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yuge Ran,Shuai Han,Dongxue Gao,Xiaobo Chen,Chan Liu

Cell reports 43:113680 PubMed38241148

2024

Notch receptor-ligand binding facilitates extracellular vesicle-mediated neuron-to-neuron communication.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Zhi Wang,Charlotte C M Castillon,Kamil K Gebis,Elizabeth T Bartom,Alessandra d'Azzo,Anis Contractor,Jeffrey N Savas

Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan 43:955-962 PubMed37679983

2023

Efficacy of electroacupuncture stimulating Zusanli (ST36) and Xuanzhong (GB39) on synovial angiogenesis in rats with adjuvant arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiang Jianzhen,Zhang Xin,Luo Zhenguo,S U Chengguo,Zhou Haiyan,Jiang Yuqing,Xiao Xianjun,Chen Yunfei,Zhu Jun

Frontiers in immunology 14:1127612 PubMed37622120

2023

GBP2 promotes M1 macrophage polarization by activating the notch1 signaling pathway in diabetic nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohui Li,Jialu Liu,Mengru Zeng,Kexin Yang,Shumin Zhang,Yifei Liu,Xiangxiang Yin,Chanyue Zhao,Wenpeng Wang,Li Xiao

International journal of chronic obstructive pulmonary disease 18:919-932 PubMed37229441

2023

Effect of the Lipoxin Receptor Agonist BML-111 on Cigarette Smoke Extract-Induced Macrophage Polarization and Inflammation in RAW264.7 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

En Cao,Jun Xu,Yuanqi Gong,Jingjing Yuan,Anbang Chen,Jiayi Liu,Yunfei Fan,Xiangyang Fan,Xiaodong Kuang

Frontiers in genetics 14:1148126 PubMed37284062

2023

Development and validation of an immune infiltration/tumor proliferation-related Notch3 nomogram for predicting survival in patients with primary glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zong-Qing Zheng,Guo-Guo Zhang,Gui-Qiang Yuan,Jia-Hui Hao,Qian-Qian Nie,Ming-Cheng Zheng,Zhong Wang

PeerJ 11:e14950 PubMed36908813

2023

Abnormal mechanical stress on bicuspid aortic valve induces valvular calcification and inhibits Notch1/NICD/Runx2 signal.

Applications

Unspecified application

Species

Unspecified reactive species

Guangzhou Li,Na Shen,Huifang Deng,Yixuan Wang,Gangcheng Kong,Jiawei Shi,Nianguo Dong,Cheng Deng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-activated Notch1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-activated Notch1 antibody (AB52301)

Western blot - Anti-activated Notch1 antibody (AB52301)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (65)

Product promise