Rabbit Polyclonal Notch1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 53 publications. Immunogen corresponding to Synthetic Peptide within Human NOTCH1 aa 1700-1800.
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- Frontiers in immunology 14:11276122023GBP2 promotes M1 macrophage polarization by activating the notch1 signaling pathway in diabetic nephropathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaohui Li,Jialu Liu,Mengru Zeng,Kexin Yang,Shumin Zhang,Yifei Liu,Xiangxiang Yin,Chanyue Zhao,Wenpeng Wang,Li XiaoPubMed 37622120
- International journal of chronic obstructive pulmonary disease 18:919-9322023Effect of the Lipoxin Receptor Agonist BML-111 on Cigarette Smoke Extract-Induced Macrophage Polarization and Inflammation in RAW264.7 Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEn Cao,Jun Xu,Yuanqi Gong,Jingjing Yuan,Anbang Chen,Jiayi Liu,Yunfei Fan,Xiangyang Fan,Xiaodong KuangPubMed 37229441
- Frontiers in genetics 14:11481262023Development and validation of an immune infiltration/tumor proliferation-related Notch3 nomogram for predicting survival in patients with primary glioblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZong-Qing Zheng,Guo-Guo Zhang,Gui-Qiang Yuan,Jia-Hui Hao,Qian-Qian Nie,Ming-Cheng Zheng,Zhong WangPubMed 37284062
- Clinical and experimental rheumatology 41:902-9092023AMPK hyperactivity maintains the survival of vasculogenic T cells in patients with Takayasu's arteritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengyao Sun,Xin Zhang,Yuanyuan Hao,Pengcheng Yin,Jinghai Hu,Ming Zheng,Zhenke Wen,Xiyu Liu,Chunxi WangPubMed 36826789
- The Journal of neuroscience : the official journal of the Society for Neuroscience 42:7031-70462022Functional Cooperation of α-Synuclein and Tau Is Essential for Proper Corticogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShengming Wang,Yu Fu,Takaki Miyata,Sakiko Matsumoto,Tomoyasu Shinoda,Kyoko Itoh,Akihiro Harada,Shinji Hirotsune,Mingyue JinPubMed 35906071
- Frontiers in pharmacology 13:7978052022Atractylenolide III Attenuates Angiogenesis in Gastric Precancerous Lesions Through the Downregulation of Delta-Like Ligand 4.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Gao,Jundong Wang,Maoyuan Zhao,Ting Xia,Qingsong Liu,Nianzhi Chen,Wenhao Liao,Zhongzhen Zeng,Fengming You,Jinhao ZengPubMed 35846998
- Arthritis & rheumatology (Hoboken, N.J.) 74:1235-12442022Critical Role of Notch-1 in Mechanistic Target of Rapamycin Hyperactivity and Vascular Inflammation in Patients With Takayasu Arteritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWanwan Jiang,Mengyao Sun,Ying Wang,Ming Zheng,Zixin Yuan,Shixiong Mai,Xin Zhang,Longhai Tang,Xiyu Liu,Chunhong Wang,Zhenke WenPubMed 35212196
- Cancer management and research 13:7225-72342021SOX10 Knockdown Inhibits Melanoma Cell Proliferation via Notch Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYouqun Tang,Yanming CaoPubMed 34557039
- Stem cells international 2021:88590712021Inhibition of Notch Signaling Promotes the Differentiation of Epicardial Progenitor Cells into Adipocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBin Liu,Dinghui Wang,Tianhua Xiong,Yajie Liu,Xiaodong Jing,Jianlin Du,Qiang ShePubMed 33897781
- Ecotoxicology and environmental safety 203:1109562020The effect of Notch signal pathway on PM-induced Muc5ac in Beas-2B cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Liu et. al.PubMed 32678753
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human NOTCH1 aa 1700-1800. The exact immunogen used to generate this antibody is proprietary information. Database link P46531
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Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4(+) and CD8(+) cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved in mesoderm development, somite formation and neurogenesis. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Required for the THBS4 function in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury. Involved in determination of left/right symmetry by modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO).
Alternative names
TAN1, NOTCH1, Neurogenic locus notch homolog protein 1, Notch 1, hN1, Translocation-associated notch protein TAN-1
Recommended products
Rabbit Polyclonal Notch1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 53 publications. Immunogen corresponding to Synthetic Peptide within Human NOTCH1 aa 1700-1800.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human NOTCH1 aa 1700-1800. The exact immunogen used to generate this antibody is proprietary information. Database link P46531
- Purification technique
- Affinity purification Immunogen
- Specificity
ab52301 detects endogenous levels of fragment of activated Notch 1 resulting from cleavage adjacent to Val1744.
- Concentration
- Purification notes
ab52301 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
ab52301 is cleaved at Val 1744
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Activated Notch1 also known as cleaved Notch1 and Notch 1 NICD is an intracellular domain resulting from Notch1 receptor cleavage. The molecular weight of Notch1 NICD varies but it is generally around 120-150 kDa depending on post-translational modifications. These receptors are present in many cell types including embryonic cells stem cells and various tissue-specific cells. They reside in the cell membrane and become activated upon interaction with specific ligands.
- Biological function summary
Activated Notch1 is critical for regulating cell fate decisions including proliferation differentiation and apoptosis. It participates in a multi-protein complex that includes the CSL (CBF1/Su(H)/LAG-1) transcription factor modulating gene expression by binding to DNA. The role of activated Notch1 in maintaining the balance between cell growth and death is important in tissue homeostasis and development.
- Pathways
Activated Notch1 plays a central role in the Notch signaling pathway an essential pathway for intercellular communication. This pathway involves several proteins including Delta and Jagged as ligands. It contributes to several critical developmental processes and is connected with the regulation of the NF-kB signaling pathway involved in immune responses and inflammation.
- Associated diseases and disorders
Activated Notch1 is significantly associated with T-cell acute lymphoblastic leukemia (T-ALL) and breast cancer. Abnormal expressions or mutations in Notch1 can lead to unchecked cellular proliferation in these conditions. Notch1 alterations often coexist with disruptions in other signaling proteins such as p53 contributing to disease pathology and presenting potential targets for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-activated Notch1 antibody (ab52301)
All lanes: Western blot - Anti-activated Notch1 antibody (ab52301) at 1/500 dilution
Lane 1: NIH 3T3 cell extract treated with etoposide (25µM, 1 hour)
Lane 2: NIH 3T3 cell extract treated with etoposide (25µM, 1 hour) with immunising peptide
Predicted band size: 273 kDa
Observed band size: 95 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-activated Notch1 antibody (ab52301)
Immunohistochemical analysis of paraffin-embedded human brain tissue sections labeling activated Notch1 with ab52301 at a dilution of 1/100. The picture on the right is treated with the synthesized peptide.
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