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Anti-active YAP1 antibody [EPR19812] ab205270 is a rabbit monoclonal antibody that is used in active YAP1 western blotting, IHC and immunofluorescence. Suitable for human and mouse samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity confirmed with YAP1 knockout cell line validation
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- Antibody clone EPR19812 is cited in over 130 publications

New 20 ul size available


Images

Western blot - Anti-active YAP1 antibody [EPR19812] (AB205270), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-active YAP1 antibody [EPR19812] (AB205270), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-active YAP1 antibody [EPR19812] (AB205270), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-active YAP1 antibody [EPR19812] (AB205270), expandable thumbnail
  • Western blot - Anti-active YAP1 antibody [EPR19812] (AB205270), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-P
Human
Tested
Tested
Tested
Mouse
Tested
Expected
Tested

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Transcriptional regulator with dual roles as a coactivator and corepressor. Critical downstream regulatory target in the Hippo signaling pathway, crucial for organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed:17974916, PubMed:18280240, PubMed:18579750, PubMed:21364637, PubMed:30447097). The Hippo signaling pathway core involves a kinase cascade featuring STK3/MST2 and STK4/MST1, along with its regulatory partner SAV1, which phosphorylates and activates LATS1/2 in complex with their regulatory protein, MOB1. This activation leads to the phosphorylation and inactivation of the YAP1 oncoprotein and WWTR1/TAZ (PubMed:18158288). Phosphorylation of YAP1 by LATS1/2 prevents its nuclear translocation, thereby regulating the expression of its target genes (PubMed:18158288, PubMed:26598551, PubMed:34404733). The transcriptional regulation of gene expression requires TEAD transcription factors and modulates cell growth, anchorage-independent growth, and induction of epithelial-mesenchymal transition (EMT) (PubMed:18579750). Plays a key role in tissue tension and 3D tissue shape by regulating the cortical actomyosin network, acting via ARHGAP18, a Rho GTPase activating protein that suppresses F-actin polymerization (PubMed:25778702). It also suppresses ciliogenesis by acting as a transcriptional corepressor of TEAD4 target genes AURKA and PLK1 (PubMed:25849865). In conjunction with WWTR1, regulates TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation (By similarity). Synergizes with WBP2 to enhance PGR activity (PubMed:16772533).Isoform 2Activates the C-terminal fragment (CTF) of ERBB4 (isoform 3).Isoform 3Activates the C-terminal fragment (CTF) of ERBB4 (isoform 3).

Alternative names

YAP65, YAP65, YAP1, Transcriptional coactivator YAP1, Yes-associated protein 1, Protein yorkie homolog, Yes-associated protein YAP65 homolog

Recommended products

Anti-active YAP1 antibody [EPR19812] ab205270 is a rabbit monoclonal antibody that is used in active YAP1 western blotting, IHC and immunofluorescence. Suitable for human and mouse samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity confirmed with YAP1 knockout cell line validation
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- Antibody clone EPR19812 is cited in over 130 publications

New 20 ul size available

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR19812

Purification technique

Affinity purification Protein A

Specificity

ab205270 is specific to the active (non-phosphorylated) form of YAP1.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Active YAP1 also known as Yes-associated protein 1 is a transcription co-activator with a mass of approximately 65 kDa. It plays a mechanical role in the regulation of gene expression by interacting with transcription factors and enhancing their ability to drive transcription. YAP1 is expressed in various tissues including the liver intestine and neural tissues. Also found in the cytoplasm and nucleus its expression is tightly regulated because of its involvement in maintaining cell proliferation and survival.

Biological function summary

Active YAP1 functions as an important regulator in the Hippo signaling pathway. It acts as an effector that transmits signals from upstream kinases to control organ size by modulating cell growth and apoptosis. YAP1 associates with the TEAD transcription factor family to regulate the expression of genes involved in cell proliferation and differentiation. The complex formed between YAP1 and TEAD is essential for its biological activity influencing processes like stem cell renewal and tissue regeneration.

Pathways

Active YAP1 has a central role in the Hippo signaling pathway important for controlling cellular behaviors such as growth and survival. In this pathway YAP1 interacts with other proteins like LATS1/2 kinases to control its activity through phosphorylation and subcellular localization. Akt/mTOR pathway also connects with YAP1 integrating signals that affect cell growth and metabolism. These interactions highlight YAP1’s position as a pivotal node in maintaining cellular homeostasis.

Associated diseases and disorders

Active YAP1 is implicated in several conditions including cancer and liver disorders. Overexpression or dysregulation of YAP1 leads to oncogenic transformation closely associating it with cancers such as hepatocellular carcinoma and breast cancer. In liver disorders YAP1's activity affects fibrosis and regeneration by interacting with proteins like β-catenin modulating pathways linked to tissue repair and scarring. Understanding YAP1’s role offers potential therapeutic targets for managing these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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16 product images

  • Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270), expandable thumbnail

    Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270)

    Lanes 1 - 3: Merged signal (red and green). Green - ab205270 observed at 54 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.

    ab205270 was shown to recognize active YAP1 in wild-type HAP1 cells as signal was lost at the expected MW in active YAP1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and active YAP1 knockout samples were subjected to SDS-PAGE. ab205270 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270) at 1 µg/mL

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: YAP1 knockout HAP1 whole cell lysate at 20 µg

    Lane 3: HeLa whole cell lysate at 20 µg

    Predicted band size: 54 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-active YAP1 antibody [EPR19812] (ab205270), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-active YAP1 antibody [EPR19812] (ab205270)

    Immunohistochemical analysis of paraffin-embedded human breast tissue labeling active YAP1 with ab205270 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Mainly nuclear staining on human breast is observed [PMID: 18617895].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-active YAP1 antibody [EPR19812] (ab205270), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-active YAP1 antibody [EPR19812] (ab205270)

    ab205270 staining active YAP1 in HUVEC (human umbilical vein endothelial cell) cells by Immunocytochemistry/Immunofluorescence (ICC/IF). The cells were fixed 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Samples were incubated with primary antibody at 1:500 dilution. An Alexa Fluor® 488 Goat anti-Rabbit was used as a secondary antibody at 1:1000 dilution. DAPI was used as a nuclear counter stain. Confocal image showing nuclear and cytoplasmic staining in HUVEC cells.

  • Immunocytochemistry/ Immunofluorescence - Anti-active YAP1 antibody [EPR19812] (ab205270), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-active YAP1 antibody [EPR19812] (ab205270)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton/PBS permeabilized (RT, 5 mins) 293A (Human epithelial cell line from embryonic kidney transformed with sheared human adenovirus type 5 DNA) cells labeling active YAP1 with ab205270 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 594) secondary antibody at 1/1000 dilution.

    The images showed weak staining on 293A cells under serum starvation overnight. After 10% FBS was added to the medium for 1h, the nuclear staining was increased.

    The data was kindly provided by our collaborator Dr. Bin Zhao (Zhejiang University).

    The nuclear counterstain is DAPI (blue). Counterstained with Phalloidin-technology® Alexa Fluor 488 at 1/1000 dilution.

  • Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270), expandable thumbnail

    Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270)

    Blocking buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270) at 1/1000 dilution

    Lane 1: Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

    Lane 2: HeLa treated with 100ng/ml Calyculin A for 30 min whole cell lysate at 15 µg

    Lane 3: HeLa treated with 100ng/ml Calyculin A for 30 min whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 54 kDa

    Observed band size: 75 kDa

    Exposure time: 80s

  • Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270), expandable thumbnail

    Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270)

    Blocking buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270) at 1/1000 dilution

    Lane 1: Untreated NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 15 µg

    Lane 2: NIH/3T3 treated with 100ng/ml Calyculin A for 30 min whole cell lysate at 15 µg

    Lane 3: NIH/3T3 treated with 100ng/ml Calyculin A for 30 min whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 54 kDa

    Observed band size: 75 kDa

    Exposure time: 20s

  • Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270), expandable thumbnail

    Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Serum starvation induces active YAP1 Ser127 phosphorylation. The level of active YAP1 protein is inversely proportional to p-YAP1 Ser127 level (PMID: 22884261).

    All lanes: Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270) at 1/1000 dilution

    Lane 1: 293A cells under serum starvation overnight lysate at 20 µg

    Lane 2: 293A cell lysate under serum starvation overnight, then 10% FBS was added to medium for 1 hour at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 54 kDa

    Observed band size: 75 kDa

    Exposure time: 30s

  • Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270), expandable thumbnail

    Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1/2: 8 seconds; Lane 3: 30 seconds.

    All lanes: Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270) at 1/1000 dilution

    Lane 1: Human kidney lysate at 10 µg

    Lane 2: Human skin lysate at 10 µg

    Lane 3: HaCaT (Human keratinocyte cell line) whole cell lysate at 10 µg

    Secondary

    Lanes 1 - 2: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Lane 3: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 54 kDa

    Observed band size: 75 kDa

  • Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270), expandable thumbnail

    Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 10 seconds; Lane 2: 8 seconds; Lane 3: 3 minutes.

    All lanes: Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270) at 1/1000 dilution

    Lane 1: Mouse testis lysate at 10 µg

    Lane 2: Mouse skin lysate at 10 µg

    Lane 3: Mouse liver lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 52 kDa, 54 kDa

    Observed band size: 52 kDa, 75 kDa

  • Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270), expandable thumbnail

    Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270)

    Blocking/Dilution buffer: 5% BSA/TBST.

    The two panels for pYAPS127 were just shorter and longer exposure.

    The data was kindly provided by our collaborator Dr. Bin Zhao (Zhejiang University).

    All lanes: Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270) at 1/1000 dilution

    Lane 1: 293A cell lysate serum starved overnight and then 10% FBS added to the medium for 1 hour at 10 µg

    Lane 2: 293A cell lysate serum starved overnight at 10 µg

    Lane 3: 293A cell lysate serum starved overnight and then treated with Lambda phosphatase at 10 µg

    Secondary

    All lanes: donkey anti-rabbit at 1/1000 dilution

    Predicted band size: 54 kDa

    Observed band size: 75 kDa

    Exposure time: 30s

  • Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270), expandable thumbnail

    Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270)

    Blocking/Dilution buffer: 5% BSA/TBST.

    The data was kindly provided by our collaborator Dr. Bin Zhao (Zhejiang University).

    All lanes: Western blot - Anti-active YAP1 antibody [EPR19812] (ab205270) at 1/1000 dilution

    Lane 1: 293A cell lysate at 20 µg

    Lane 2: YAP/TAZ knockout 293A cell lysate at 20 µg

    Secondary

    All lanes: donkey anti-rabbit at 1/1000 dilution

    Predicted band size: 54 kDa

    Observed band size: 75 kDa

    Exposure time: 30s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-active YAP1 antibody [EPR19812] (ab205270), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-active YAP1 antibody [EPR19812] (ab205270)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling active YAP1 with ab205270 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Nuclear and cytoplasmic staining on human breast cancer is observed [PMID: 24559095].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-active YAP1 antibody [EPR19812] (ab205270), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-active YAP1 antibody [EPR19812] (ab205270)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling active YAP1 with ab205270 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Mainly nuclear staining on mouse skin is observed [PMID: 21610251].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-active YAP1 antibody [EPR19812] (ab205270), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-active YAP1 antibody [EPR19812] (ab205270)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton/PBS permeabilized (RT, 5 mins) 293A (Human epithelial cell line from embryonic kidney transformed with sheared human adenovirus type 5 DNA) cells labeling active YAP1 with ab205270 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 594) secondary antibody at 1/1000 dilution.

    The images showed nuclear staining on 293A cells, and background staining on YAP/TAZ knockout 293A cells.

    The data was kindly provided by our collaborator Dr. Bin Zhao (Zhejiang University).

    The nuclear counterstain is DAPI (blue). Counterstained with Phalloidin-technology® Alexa Fluor 488 at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-active YAP1 antibody [EPR19812] (ab205270), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-active YAP1 antibody [EPR19812] (ab205270)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling active YAP1 with ab205270 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Negative control: no staining on human liver [PMID:17974916].

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry - Anti-active YAP1 antibody [EPR19812] (ab205270), expandable thumbnail

    Immunohistochemistry - Anti-active YAP1 antibody [EPR19812] (ab205270)

    Immunohistochemical analysis of formalin fixed paraffin embedded human colon labelling active YAP1 with ab205270 at a concentration of 0.09 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH 8.5 for 32 mins.

    ab205270 Anti-active YAP1 antibody [EPR19812] was incubated for 16 mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

    Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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