AB236051

Anti-Activin Receptor Type IA antibody [EPR4076(2)] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Activin Receptor Type IA antibody. Carrier free. Suitable for IP, WB and reacts with Mouse, Human, Rat samples. Cited in 1 publication.

View Alternative Names

ACVRLK2, ACVR1, Activin receptor type-1, Activin receptor type I, Activin receptor-like kinase 2, Serine/threonine-protein kinase receptor R1, TGF-B superfamily receptor type I, ACTR-I, ALK-2, SKR1, TSR-I

2 Images

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Immunoprecipitation - Anti-Activin Receptor Type IA antibody [EPR4076(2)] - BSA and Azide free (AB236051)

ab155981 (purified) at 1 : 50 dilution (2μg) immunoprecipitating Activin Receptor Type IA in Mouse brain lysate.
Lane 1 (input) : Mouse brain lysate 10μg
Lane 2 (+) : ab155981 & Mouse brain lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab155981 in Mouse brain lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155981).

All lanes:

Immunoprecipitation - Anti-Activin Receptor Type IA antibody [EPR4076(2)] (<a href='/en-us/products/primary-antibodies/activin-receptor-type-ia-antibody-epr40762-ab155981'>ab155981</a>)

Predicted band size: 43 kDa,57 kDa

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Supplier Data

Western blot - Anti-Activin Receptor Type IA antibody [EPR4076(2)] - BSA and Azide free (AB236051)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155981).

All lanes:

Western blot - Anti-Activin Receptor Type IA antibody [EPR4076(2)] (<a href='/en-us/products/primary-antibodies/activin-receptor-type-ia-antibody-epr40762-ab155981'>ab155981</a>) at 1/1000 dilution

Lane 1:

Fetal brain lysate at 10 µg

Lane 2:

Fetal heart lysate at 10 µg

Lane 3:

Fetal muscle lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 57 kDa

Observed band size: 57 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4076(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab236051 is the carrier-free version of ab155981.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Activin Receptor Type IA also known as ACVR1 or ALK2 is a type I receptor serine/threonine kinase. It has a mass of approximately 55 kDa. Activin Receptor Type IA is widely expressed in various tissues including skeletal muscle heart and central nervous system. It functions through the binding of ligands like activins and bone morphogenetic proteins (BMPs) which triggers intracellular signaling cascades important for cellular processes.

Biological function summary

Activin Receptor Type IA plays a significant role in several cellular activities including growth and differentiation. It operates as part of a receptor complex with type II receptors like ACVR2A protein which facilitates ligand binding and phosphorylation. Activin receptor is essential for the regulation of mesoderm induction and organ development notably in the skeletal and muscular systems. Its interaction with activin protein family members influences various developmental processes.

Pathways

Activin Receptor Type IA is an integral component of the TGF-beta signaling pathway and BMP pathway. These pathways are vital for embryonic development and tissue homeostasis. Activin receptor interacts with the SMAD family of proteins which mediate signal transduction to the nucleus influencing gene expression. Through these pathways it relates functionally to SMAD1 and SMAD2 proteins that contribute to cell proliferation and differentiation dynamics.

Activin Receptor Type IA is associated with fibrodysplasia ossificans progressiva (FOP) and cancer. Mutations in the ACVR1 gene can lead to abnormal bone growth seen in FOP commonly due to erroneous activation of the BMP signaling pathway. In some cancers the dysregulation of activin receptor signaling can alter cell proliferation rates placing it in connection with proteins involved in oncogenic processes. Targeting activin receptor pathways might offer therapeutic potential in certain diseases by modulating or restoring its normal signaling capability.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Bone morphogenetic protein (BMP) type I receptor that is involved in a wide variety of biological processes, including bone, heart, cartilage, nervous, and reproductive system development and regulation (PubMed : 20628059, PubMed : 22977237). As a type I receptor, forms heterotetrameric receptor complexes with the type II receptors AMHR2, ACVR2A or ACVR2B (PubMed : 17911401). Upon binding of ligands such as BMP7 or GDF2/BMP9 to the heteromeric complexes, type II receptors transphosphorylate ACVR1 intracellular domain (PubMed : 25354296). In turn, ACVR1 kinase domain is activated and subsequently phosphorylates SMAD1/5/8 proteins that transduce the signal (PubMed : 9748228). In addition to its role in mediating BMP pathway-specific signaling, suppresses TGFbeta/activin pathway signaling by interfering with the binding of activin to its type II receptor (PubMed : 17911401). Besides canonical SMAD signaling, can activate non-canonical pathways such as p38 mitogen-activated protein kinases/MAPKs (By similarity). May promote the expression of HAMP, potentially via its interaction with BMP6 (By similarity).

See full target information ACVR1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Burns & trauma 13:tkae065 PubMed40040959

2025

Single-cell RNA sequencing reveals the impaired epidermal differentiation and pathological microenvironment in diabetic foot ulcer.

Applications

Unspecified application

Species

Unspecified reactive species

Yiling Liu,Peng Wang,Jingting Li,Lei Chen,Bin Shu,Hanwen Wang,Hengdeng Liu,Shixin Zhao,Junli Zhou,Xiaodong Chen,Julin Xie

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Activin Receptor Type IA antibody [EPR4076(2)] - BSA and Azide free

Immunoprecipitation - Anti-Activin Receptor Type IA antibody [EPR4076(2)] - BSA and Azide free (AB236051)

Western blot - Anti-Activin Receptor Type IA antibody [EPR4076(2)] - BSA and Azide free (AB236051)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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