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Rabbit Recombinant Monoclonal Activin A Receptor Type IB/ALK-4 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human Activin receptor type-1B.

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Images

Western blot - Anti-Activin Receptor Type IB/BALK-4 antibody [HL1266] - BSA and Azide free (AB313839), expandable thumbnail
  • Western blot - Anti-Activin Receptor Type IB/BALK-4 antibody [HL1266] - BSA and Azide free (AB313839), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human Activin receptor type-1B. Database link P36896

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested

Tested
Tested

Species

Human

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Species

Mouse

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Target data

Function

Transmembrane serine/threonine kinase activin type-1 receptor forming an activin receptor complex with activin receptor type-2 (ACVR2A or ACVR2B). Transduces the activin signal from the cell surface to the cytoplasm and is thus regulating a many physiological and pathological processes including neuronal differentiation and neuronal survival, hair follicle development and cycling, FSH production by the pituitary gland, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. Activin is also thought to have a paracrine or autocrine role in follicular development in the ovary. Within the receptor complex, type-2 receptors (ACVR2A and/or ACVR2B) act as a primary activin receptors whereas the type-1 receptors like ACVR1B act as downstream transducers of activin signals. Activin binds to type-2 receptor at the plasma membrane and activates its serine-threonine kinase. The activated receptor type-2 then phosphorylates and activates the type-1 receptor such as ACVR1B. Once activated, the type-1 receptor binds and phosphorylates the SMAD proteins SMAD2 and SMAD3, on serine residues of the C-terminal tail. Soon after their association with the activin receptor and subsequent phosphorylation, SMAD2 and SMAD3 are released into the cytoplasm where they interact with the common partner SMAD4. This SMAD complex translocates into the nucleus where it mediates activin-induced transcription. Inhibitory SMAD7, which is recruited to ACVR1B through FKBP1A, can prevent the association of SMAD2 and SMAD3 with the activin receptor complex, thereby blocking the activin signal. Activin signal transduction is also antagonized by the binding to the receptor of inhibin-B via the IGSF1 inhibin coreceptor. ACVR1B also phosphorylates TDP2.

Targets

Acvr1b

Alternative names

Recommended products

Rabbit Recombinant Monoclonal Activin A Receptor Type IB/ALK-4 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human Activin receptor type-1B.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Fragment Protein within Human Activin receptor type-1B. Database link P36896
Carrier free

Yes

Clone number

HL1266

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Activin A Receptor Type IB also known as ACVR1B or ALK-4 is a serine/threonine kinase involved in the TGF-beta superfamily signaling. This receptor has a molecular mass of approximately 54 kDa and is expressed in a variety of tissues including the brain heart and reproductive organs. ALK-4 specifically interacts with type II activin receptors to mediate the signal transduction processes that are critical for cell differentiation and growth.

Biological function summary

ALK-4 plays an important role in cell differentiation proliferation and apoptosis. It forms a complex with activin type II receptors which is essential for its proper function. This receptor mediates the signaling pathways that regulate these cellular processes having a significant impact on development and tissue homeostasis. The action of ALK-4 is important for the regulation of mesodermal differentiation and the formation of the axial skeleton during embryogenesis.

Pathways

ALK-4 is part of the TGF-beta signaling pathway and the activin signaling pathway. These pathways are vital for various cellular processes including immune response and tissue repair. In the activin pathway ALK-4 functions along with its related proteins such as ACVR2A and SMAD2/3 to propagate the signal that induces gene expression changes. This signaling cascade involves phosphorylation events that eventually lead to the regulation of target gene expression in the nucleus.

Associated diseases and disorders

ALK-4 has associations with reproductive disorders and cancer. Dysregulation of activin signaling involving ALK-4 can contribute to disorders such as polycystic ovary syndrome (PCOS) and has implications in tumor progression and metastasis in cancer. The receptor works closely with other proteins like inhibin and follistatin which modulate activin signaling highlighting its role in pathologies related to aberrant cell proliferation and differentiation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-Activin Receptor Type IB/BALK-4 antibody [HL1266] - BSA and Azide free (ab313839), expandable thumbnail

    Western blot - Anti-Activin Receptor Type IB/BALK-4 antibody [HL1266] - BSA and Azide free (ab313839)

    All lanes: Western blot - Anti-Activin Receptor Type IB/BALK-4 antibody [HL1266] - BSA and Azide free (ab313839) at 1/1000 dilution

    Lane 1: Mouse kidney tissue extract at 30 µg

    Lane 2: Mouse spleen tissue extract at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Developed using the ECL technique.

    Predicted band size: 57 kDa

  • Western blot - Anti-Activin Receptor Type IB/BALK-4 antibody [HL1266] - BSA and Azide free (ab313839), expandable thumbnail

    Western blot - Anti-Activin Receptor Type IB/BALK-4 antibody [HL1266] - BSA and Azide free (ab313839)

    All lanes: Western blot - Anti-Activin Receptor Type IB/BALK-4 antibody [HL1266] - BSA and Azide free (ab313839) at 1/1000 dilution

    Lane 1: Caco-2 (Human colorectal adenocarcinoma cell line) whole cell extract at 30 µg

    Lane 2: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Developed using the ECL technique.

    Predicted band size: 57 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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