Rabbit Recombinant Monoclonal Activin A Receptor Type IB/ALK-4 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human Activin receptor type-1B.
IgG
Rabbit
pH: 7.4
Constituents: 100% PBS
Liquid
Monoclonal
WB | |
---|---|
Human | Tested |
Mouse | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Transmembrane serine/threonine kinase activin type-1 receptor forming an activin receptor complex with activin receptor type-2 (ACVR2A or ACVR2B). Transduces the activin signal from the cell surface to the cytoplasm and is thus regulating a many physiological and pathological processes including neuronal differentiation and neuronal survival, hair follicle development and cycling, FSH production by the pituitary gland, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. Activin is also thought to have a paracrine or autocrine role in follicular development in the ovary. Within the receptor complex, type-2 receptors (ACVR2A and/or ACVR2B) act as a primary activin receptors whereas the type-1 receptors like ACVR1B act as downstream transducers of activin signals. Activin binds to type-2 receptor at the plasma membrane and activates its serine-threonine kinase. The activated receptor type-2 then phosphorylates and activates the type-1 receptor such as ACVR1B. Once activated, the type-1 receptor binds and phosphorylates the SMAD proteins SMAD2 and SMAD3, on serine residues of the C-terminal tail. Soon after their association with the activin receptor and subsequent phosphorylation, SMAD2 and SMAD3 are released into the cytoplasm where they interact with the common partner SMAD4. This SMAD complex translocates into the nucleus where it mediates activin-induced transcription. Inhibitory SMAD7, which is recruited to ACVR1B through FKBP1A, can prevent the association of SMAD2 and SMAD3 with the activin receptor complex, thereby blocking the activin signal. Activin signal transduction is also antagonized by the binding to the receptor of inhibin-B via the IGSF1 inhibin coreceptor. ACVR1B also phosphorylates TDP2.
Acvr1b
ACVRLK4, ALK4, ALK4, ACVRLK4, ACVR1B, Activin receptor type-1B, Activin receptor type IB, Activin receptor-like kinase 4, Serine/threonine-protein kinase receptor R2, ACTR-IB, ALK-4, SKR2
Rabbit Recombinant Monoclonal Activin A Receptor Type IB/ALK-4 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human Activin receptor type-1B.
IgG
Rabbit
pH: 7.4
Constituents: 100% PBS
Liquid
Monoclonal
Yes
HL1266
Affinity purification Protein A
Blue Ice
+4°C
+4°C
Upon delivery aliquot
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This supplementary information is collated from multiple sources and compiled automatically.
Activin A Receptor Type IB also known as ACVR1B or ALK-4 is a serine/threonine kinase involved in the TGF-beta superfamily signaling. This receptor has a molecular mass of approximately 54 kDa and is expressed in a variety of tissues including the brain heart and reproductive organs. ALK-4 specifically interacts with type II activin receptors to mediate the signal transduction processes that are critical for cell differentiation and growth.
ALK-4 plays an important role in cell differentiation proliferation and apoptosis. It forms a complex with activin type II receptors which is essential for its proper function. This receptor mediates the signaling pathways that regulate these cellular processes having a significant impact on development and tissue homeostasis. The action of ALK-4 is important for the regulation of mesodermal differentiation and the formation of the axial skeleton during embryogenesis.
ALK-4 is part of the TGF-beta signaling pathway and the activin signaling pathway. These pathways are vital for various cellular processes including immune response and tissue repair. In the activin pathway ALK-4 functions along with its related proteins such as ACVR2A and SMAD2/3 to propagate the signal that induces gene expression changes. This signaling cascade involves phosphorylation events that eventually lead to the regulation of target gene expression in the nucleus.
ALK-4 has associations with reproductive disorders and cancer. Dysregulation of activin signaling involving ALK-4 can contribute to disorders such as polycystic ovary syndrome (PCOS) and has implications in tumor progression and metastasis in cancer. The receptor works closely with other proteins like inhibin and follistatin which modulate activin signaling highlighting its role in pathologies related to aberrant cell proliferation and differentiation.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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All lanes: Western blot - Anti-Activin Receptor Type IB/BALK-4 antibody [HL1266] - BSA and Azide free (ab313839) at 1/1000 dilution
Lane 1: Mouse kidney tissue extract at 30 µg
Lane 2: Mouse spleen tissue extract at 30 µg
All lanes: HRP-conjugated anti-rabbit IgG antibody
Developed using the ECL technique.
Predicted band size: 57 kDa
All lanes: Western blot - Anti-Activin Receptor Type IB/BALK-4 antibody [HL1266] - BSA and Azide free (ab313839) at 1/1000 dilution
Lane 1: Caco-2 (Human colorectal adenocarcinoma cell line) whole cell extract at 30 µg
Lane 2: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg
All lanes: HRP-conjugated anti-rabbit IgG antibody
Developed using the ECL technique.
Predicted band size: 57 kDa
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