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AB248675

Anti-Activin Receptor Type IIA/ACVR2A antibody [EPR7407] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Activin Receptor Type IIA/ACVR2A antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ACVR2, ACVR2A, Activin receptor type-2A, Activin receptor type IIA, ACTR-IIA, ACTRIIA

2 Images
Western blot - Anti-Activin Receptor Type IIA/ACVR2A antibody [EPR7407] - BSA and Azide free (AB248675)
  • WB

Unknown

Western blot - Anti-Activin Receptor Type IIA/ACVR2A antibody [EPR7407] - BSA and Azide free (AB248675)

This data was developed using ab134082, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Activin Receptor Type IIA/ACVR2A antibody [EPR7407] (<a href='/en-us/products/primary-antibodies/activin-receptor-type-iia-acvr2a-antibody-epr7407-ab134082'>ab134082</a>) at 1/1000 dilution

Lane 1:

Human fetal liver lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

K562 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-rabbit at 1/2000 dilution

Predicted band size: 58 kDa

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OI-RD Scanning - Anti-Activin Receptor Type IIA/ACVR2A antibody [EPR7407] - BSA and Azide free (AB248675)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Activin Receptor Type IIA/ACVR2A antibody [EPR7407] - BSA and Azide free (AB248675)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Unconjugated

    Anti-Activin Receptor Type IIA/ACVR2A antibody [EPR7407]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7407

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab248675 is the carrier-free version of ab134082.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Activin Receptor Type IIA also known as ACVR2A or ACVR2 is an important component in the activin signaling pathway. This receptor is a serine/threonine kinase that binds to activins and other ligands initiating various cellular responses. The ACVR2A protein has a known mass of about 59 kDa. This receptor is widely expressed in human tissues including the brain testes and ovaries reflecting its diverse functional roles in the body.
Biological function summary

This receptor plays important roles in cellular growth differentiation and apoptosis. ACVR2A partakes in forming signaling complexes by pairing with type I receptors to propagate downstream signals upon ligand binding. This interaction activates SMAD proteins transmitting signals into the nucleus to affect gene expression patterns pivotal for development and homeostasis.

Pathways

ACVR2A significantly influences the TGF-beta signaling pathway. It works alongside other proteins like SMAD2 and SMAD3 orchestrating processes that regulate cellular proliferation and differentiation. This receptor also contributes to the activin pathway impacting tools in embryonic development and immune responses through its interactions with related proteins.

ACVR2A has notable associations with cancer and muscle wasting conditions such as cachexia. Experts study its connection with the myostatin protein where its signaling influences muscle growth and degeneration. Alterations in ACVR2A's function or expression can contribute to cancer progression and the muscle wasting commonly observed in chronic illness patients making it a target of interest for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for activin A, activin B and inhibin A (PubMed : 17911401). Mediates induction of adipogenesis by GDF6 (By similarity).
See full target information ACVR2A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 12:4513-4535 PubMed35832075

2022

Blockade of phosphotyrosine pathways suggesting SH2 superbinder as a novel therapy for pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Wang,An-Dong Liu,Qian Niu,Xiao Feng,Yuan-Yi Zheng,Shuai-Jun Chen,Hui Xu,Qian Li,Guo-Qing Hou,Xiao-Yang Bi,Yu-Zhi Lu,Pei-Pei Cheng,Li-Mei Liang,Ye-Han Jiang,Li-Qin Zhao,Fei Liu,Lin-Jie Song,Li-Ling Zhou,Ling-Yan Xiao,Feng Chen,Shawn Shun-Cheng Li,Wan-Li Ma,Xuan Cao,Hong Ye
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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