AB76940

Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11]

Name: Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (ab76940) | Abcam
Brand: Abcam Limited
SKU: AB76940
Price: 715 USD
Availability: InStock
Rating: 5 (3 reviews)

(3 Reviews)

(13 Publications)

Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (ab76940) is a mouse monoclonal antibody detecting Activin Receptor Type IIB/ACVR2B in Western Blot, Flow Cytometry, ELISA. Suitable for Human,.

- Over 10 publications
- Trusted since 2009

View Alternative Names

Activin receptor type-2B, Activin receptor type IIB, ACTR-IIB, ACVR2B

4 Images

Supplier Data

Western blot - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (AB76940)

All lanes:

Western blot - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (ab76940)

Lane 1:

IIB/ACVR2B transfected 293T cell lysate

Lane 2:

Non-transfected 293T cell lysate

Predicted band size: 58 kDa

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Flow Cytometry - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (AB76940)

Flow Cyt

Unknown

Flow Cytometry - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (AB76940)

Overlay histogram showing HeLa cells stained with ab76940 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76940, 0.5μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

This image was generated using the ascites version of the product.

ELISA - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (AB76940)

ELISA

Supplier Data

ELISA - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (AB76940)

Detection limit for recombinant GST tagged ACVR2B is 0.1 ng/ml as a capture antibody.

Supplier Data

Western blot - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (AB76940)

MW of GST tag alone : 26 kDa

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (ab76940) at 1/500 dilution

All lanes:

immunogen (GST-tagged 100aa recombinant fragment) at 0.2 µg

Secondary

All lanes:

Goat anti-Mouse IgG (H&L) HRP-conjugated at 1/5000 dilution

Predicted band size: 58 kDa

Observed band size: 37 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1C11

Isotype

IgG2a

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt, ELISA

applications

Immunogen

Recombinant Fragment Protein within Human ACVR2B aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q13705

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Macaque monkey": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (ab76940) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), ELISA in Human, samples.

What is the molecular weight of Activin Receptor Type IIB/ACVR2B?
Anti-Activin Receptor Type IIB/ACVR2B [1C11] (ab76940) specifically detects a band for Activin Receptor Type IIB/ACVR2B (UniProt: Q13705) at a molecular weight of 58kDa.

Trusted by the scientific community
Anti-Activin Receptor Type IIB/ACVR2B [1C11] (ab76940) was first used in a scientific publication in 2009 and has been cited over 10 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Activin receptor type IIB (ACVR2B) also known as activin receptor type 2B or ACTR-IIB is a transmembrane serine/threonine kinase receptor. ACVR2B primarily binds activins though it can also interact with other ligands. This receptor weighs approximately 57 kDa. ACVR2B is widely expressed in various tissues including skeletal muscle heart and nervous tissue. The receptor plays an important role in transmitting signals from extracellular ligands to intracellular pathways influencing development and cellular functions.

Biological function summary

Activin receptor type IIB participates in the regulation of muscle growth by controlling the activity of growth inhibitory proteins. The receptor forms complexes with type I receptor kinases initiating signal transduction events. ACVR2B also interfaces with the inhibin α subunit in the context of reproductive biology. Its involvement in these complexes and interactions underlines its significance in modulating physiological processes such as cell proliferation and differentiation.

Pathways

Activin receptor type IIB plays a significant role in the TGF-beta signaling pathway and the myostatin pathway. These pathways regulate important cellular processes like muscular hypertrophy and tissue homeostasis. In these pathways ACVR2B interacts with proteins such as follistatin and myostatin. Follistatin acts as a binding protein modulating the activity of members in the TGF-beta superfamily including myostatin and activins highlighting ACVR2B's involvement in maintaining cellular equilibrium.

Activin receptor type IIB has links to muscular dystrophy and cancer. In muscular dystrophy the receptor's signaling affects muscle repair and regeneration primarily through its interaction with myostatin a known negative regulator of muscle growth. In cancer aberrant signaling through ACVR2B could contribute to tumor progression by affecting cellular proliferation and differentiation rates. Understanding ACVR2B's involvement in these diseases provides insights into potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transmembrane serine/threonine kinase activin type-2 receptor forming an activin receptor complex with activin type-1 serine/threonine kinase receptors (ACVR1, ACVR1B or ACVR1c). Transduces the activin signal from the cell surface to the cytoplasm and is thus regulating many physiological and pathological processes including neuronal differentiation and neuronal survival, hair follicle development and cycling, FSH production by the pituitary gland, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. Activin is also thought to have a paracrine or autocrine role in follicular development in the ovary. Within the receptor complex, the type-2 receptors act as a primary activin receptors (binds activin-A/INHBA, activin-B/INHBB as well as inhibin-A/INHA-INHBA). The type-1 receptors like ACVR1B act as downstream transducers of activin signals. Activin binds to type-2 receptor at the plasma membrane and activates its serine-threonine kinase. The activated receptor type-2 then phosphorylates and activates the type-1 receptor. Once activated, the type-1 receptor binds and phosphorylates the SMAD proteins SMAD2 and SMAD3, on serine residues of the C-terminal tail. Soon after their association with the activin receptor and subsequent phosphorylation, SMAD2 and SMAD3 are released into the cytoplasm where they interact with the common partner SMAD4. This SMAD complex translocates into the nucleus where it mediates activin-induced transcription. Inhibitory SMAD7, which is recruited to ACVR1B through FKBP1A, can prevent the association of SMAD2 and SMAD3 with the activin receptor complex, thereby blocking the activin signal. Activin signal transduction is also antagonized by the binding to the receptor of inhibin-B via the IGSF1 inhibin coreceptor.

See full target information ACVR2B

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 120:e2210891120 PubMed36857347

2023

State- and stimulus-specific dynamics of SMAD signaling determine fate decisions in individual cells.

Applications

Unspecified application

Species

Unspecified reactive species

Stefan Bohn,Lorenz Hexemer,Zixin Huang,Laura Strohmaier,Sonja Lenhardt,Stefan Legewie,Alexander Loewer

Cancers 15: PubMed36612143

2022

Activin B and Activin C Have Opposing Effects on Prostate Cancer Progression and Cell Growth.

Applications

Unspecified application

Species

Unspecified reactive species

Karen L Reader,Simon John-McHaffie,Sylvia Zellhuber-McMillan,Tim Jowett,David G Mottershead,Heather E Cunliffe,Elspeth J Gold

Frontiers in cell and developmental biology 9:774185 PubMed35155444

2022

Myostatin Mutation Promotes Glycolysis by Increasing Phosphorylation of Phosphofructokinase Activation of PDE5A-cGMP-PKG in Cattle Heart.

Applications

Unspecified application

Species

Unspecified reactive species

Mingjuan Gu,Xinyu Zhou,Lin Zhu,Yajie Gao,Li Gao,Chunling Bai,Lei Yang,Guangpeng Li

Frontiers in bioengineering and biotechnology 8:534 PubMed32582664

2020

Automated Hypothesis Generation to Identify Signals Relevant in the Development of Mammalian Cell and Tissue Bioprocesses, With Validation in a Retinal Culture System.

Applications

Unspecified application

Species

Unspecified reactive species

Derek Toms,Abdullah Al-Ani,Saud Sunba,Qing Yun Victor Tong,Matthew Workentine,Mark Ungrin

Cells 8: PubMed31779280

2019

Expression of miRNAs from the Imprinted Locus Signals the Osteogenic Potential of Human Pluripotent Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Laetitia Barrault,Jacqueline Gide,Tingting Qing,Lea Lesueur,Jorg Tost,Jerome Alexandre Denis,Michel Cailleret,Laetitia Aubry,Marc Peschanski,Cécile Martinat,Sandrine Baghdoyan

Journal of neuropathology and experimental neurology 78:130-139 PubMed30597051

2019

Myostatin Inhibition Using ActRIIB-mFc Does Not Produce Weight Gain or Strength in the Nebulin Conditional KO Mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer A Tinklenberg,Emily M Siebers,Margaret J Beatka,Brittany A Fickau,Samuel Ayres,Hui Meng,Lin Yang,Pippa Simpson,Henk L Granzier,Michael W Lawlor

Human molecular genetics 27:638-648 PubMed29293963

2018

Myostatin inhibition using mRK35 produces skeletal muscle growth and tubular aggregate formation in wild type and TgACTA1D286G nemaline myopathy mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer A Tinklenberg,Emily M Siebers,Margaret J Beatka,Hui Meng,Lin Yang,Zizhao Zhang,Jacob A Ross,Julien Ochala,Carl Morris,Jane M Owens,Nigel G Laing,Kristen J Nowak,Michael W Lawlor

Endocrinology 158:1217-1230 PubMed28324027

2017

Follistatin Targets Distinct Pathways To Promote Brown Adipocyte Characteristics in Brown and White Adipose Tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Rajan Singh,Melissa Braga,Srinivasa T Reddy,Se-Jin Lee,Meher Parveen,Victor Grijalva,Laurent Vergnes,Shehla Pervin

The American journal of pathology 186:1568-81 PubMed27102768

2016

Treatment with ActRIIB-mFc Produces Myofiber Growth and Improves Lifespan in the Acta1 H40Y Murine Model of Nemaline Myopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer Tinklenberg,Hui Meng,Lin Yang,Fujun Liu,Raymond G Hoffmann,Mahua Dasgupta,Kenneth P Allen,Alan H Beggs,Edna C Hardeman,R Scott Pearsall,Robert H Fitts,Michael W Lawlor

Skeletal muscle 6:14 PubMed27047655

2016

Dystrophin-deficient dogs with reduced myostatin have unequal muscle growth and greater joint contractures.

Applications

Unspecified application

Species

Unspecified reactive species

Joe N Kornegay,Daniel J Bogan,Janet R Bogan,Jennifer L Dow,Jiahui Wang,Zheng Fan,Naili Liu,Leigh C Warsing,Robert W Grange,Mihye Ahn,Cynthia J Balog-Alvarez,Steven W Cotten,Monte S Willis,Candice Brinkmeyer-Langford,Hongtu Zhu,Joe Palandra,Carl A Morris,Martin A Styner,Kathryn R Wagner

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11]

Western blot - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (AB76940)

Flow Cytometry - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (AB76940)

ELISA - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (AB76940)

Western blot - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (AB76940)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

Product promise