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Mouse Monoclonal Activin Receptor Type IIB/ACVR2B antibody. Carrier free. Suitable for Flow Cyt, ELISA, WB and reacts with Human, Recombinant full length protein, Recombinant fragment - Human samples. Cited in 13 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ACVR2B aa 1-150.


Images

Key facts

Isotype

IgG2a

Host species

Mouse

Storage buffer

pH: 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human ACVR2B aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q13705
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytELISAWB
Human
Tested
Expected
Expected
Rat
Predicted
Predicted
Predicted
Chicken
Predicted
Predicted
Predicted
Chimpanzee
Predicted
Predicted
Predicted
Cow
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Horse
Predicted
Predicted
Predicted
Macaque monkey
Predicted
Predicted
Predicted
Pig
Predicted
Predicted
Predicted
Recombinant fragment - Human
Not recommended
Not recommended
Tested
Recombinant full length protein
Not recommended
Tested
Not recommended
Sheep
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

-

Notes

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species

Rat, Sheep, Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Macaque monkey

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein, Recombinant fragment - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Recombinant full length protein

Dilution info

-

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Sheep, Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Macaque monkey

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Sheep, Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Macaque monkey

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein

Dilution info

-

Notes

-

Target data

Function

Transmembrane serine/threonine kinase activin type-2 receptor forming an activin receptor complex with activin type-1 serine/threonine kinase receptors (ACVR1, ACVR1B or ACVR1c). Transduces the activin signal from the cell surface to the cytoplasm and is thus regulating many physiological and pathological processes including neuronal differentiation and neuronal survival, hair follicle development and cycling, FSH production by the pituitary gland, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. Activin is also thought to have a paracrine or autocrine role in follicular development in the ovary. Within the receptor complex, the type-2 receptors act as a primary activin receptors (binds activin-A/INHBA, activin-B/INHBB as well as inhibin-A/INHA-INHBA). The type-1 receptors like ACVR1B act as downstream transducers of activin signals. Activin binds to type-2 receptor at the plasma membrane and activates its serine-threonine kinase. The activated receptor type-2 then phosphorylates and activates the type-1 receptor. Once activated, the type-1 receptor binds and phosphorylates the SMAD proteins SMAD2 and SMAD3, on serine residues of the C-terminal tail. Soon after their association with the activin receptor and subsequent phosphorylation, SMAD2 and SMAD3 are released into the cytoplasm where they interact with the common partner SMAD4. This SMAD complex translocates into the nucleus where it mediates activin-induced transcription. Inhibitory SMAD7, which is recruited to ACVR1B through FKBP1A, can prevent the association of SMAD2 and SMAD3 with the activin receptor complex, thereby blocking the activin signal. Activin signal transduction is also antagonized by the binding to the receptor of inhibin-B via the IGSF1 inhibin coreceptor.

Alternative names

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Mouse Monoclonal Activin Receptor Type IIB/ACVR2B antibody. Carrier free. Suitable for Flow Cyt, ELISA, WB and reacts with Human, Recombinant full length protein, Recombinant fragment - Human samples. Cited in 13 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ACVR2B aa 1-150.

Alternative names

Key facts

Isotype

IgG2a

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Fragment Protein within Human ACVR2B aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q13705
Carrier free

Yes

Clone number

1C11

Purity

Tissue culture supernatant

Light chain type

kappa

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product was changed from ascites to tissue culture supernatant on 22/03/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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Supplementary info

Biological function summary

Activin receptor type IIB participates in the regulation of muscle growth by controlling the activity of growth inhibitory proteins. The receptor forms complexes with type I receptor kinases initiating signal transduction events. ACVR2B also interfaces with the inhibin α subunit in the context of reproductive biology. Its involvement in these complexes and interactions underlines its significance in modulating physiological processes such as cell proliferation and differentiation.

Activity summary

Activin receptor type IIB (ACVR2B) also known as activin receptor type 2B or ACTR-IIB is a transmembrane serine/threonine kinase receptor. ACVR2B primarily binds activins though it can also interact with other ligands. This receptor weighs approximately 57 kDa. ACVR2B is widely expressed in various tissues including skeletal muscle heart and nervous tissue. The receptor plays an important role in transmitting signals from extracellular ligands to intracellular pathways influencing development and cellular functions.

Pathways

Activin receptor type IIB plays a significant role in the TGF-beta signaling pathway and the myostatin pathway. These pathways regulate important cellular processes like muscular hypertrophy and tissue homeostasis. In these pathways ACVR2B interacts with proteins such as follistatin and myostatin. Follistatin acts as a binding protein modulating the activity of members in the TGF-beta superfamily including myostatin and activins highlighting ACVR2B's involvement in maintaining cellular equilibrium.

Associated diseases and disorders

Activin receptor type IIB has links to muscular dystrophy and cancer. In muscular dystrophy the receptor's signaling affects muscle repair and regeneration primarily through its interaction with myostatin a known negative regulator of muscle growth. In cancer aberrant signaling through ACVR2B could contribute to tumor progression by affecting cellular proliferation and differentiation rates. Understanding ACVR2B's involvement in these diseases provides insights into potential therapeutic targets.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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4 product images

  • Western blot - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (ab76940), expandable thumbnail

    Western blot - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (ab76940)

    All lanes: Western blot - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (AB76940)

    Lane 1: IIB/ACVR2B transfected 293T cell lysate

    Lane 2: Non-transfected 293T cell lysate

    Predicted band size: 58 kDa

  • Western blot - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (ab76940), expandable thumbnail

    Western blot - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (ab76940)

    MW of GST tag alone: 26 kDa

    This image was generated using the ascites version of the product.

    All lanes: Western blot - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (AB76940) at 1/500 dilution

    All lanes: immunogen (GST-tagged 100aa recombinant fragment) at 0.2 µg

    Secondary

    All lanes: Goat anti-Mouse IgG (H&L) HRP-conjugated at 1/5000 dilution

    Predicted band size: 58 kDa

    Observed band size: 37 kDa

  • ELISA - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (ab76940), expandable thumbnail

    ELISA - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (ab76940)

    Detection limit for recombinant GST tagged ACVR2B is 0.1 ng/ml as a capture antibody.

  • Flow Cytometry - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (ab76940), expandable thumbnail

    Flow Cytometry - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (ab76940)

    Overlay histogram showing HeLa cells stained with ab76940 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76940, 0.5μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

    This image was generated using the ascites version of the product.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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