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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Mouse Monoclonal Activin Receptor Type IIB/ACVR2B antibody. Carrier free. Suitable for Flow Cyt, ELISA, WB and reacts with Human, Recombinant full length protein, Recombinant fragment - Human samples. Cited in 13 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ACVR2B aa 1-150.
IgG2a
Mouse
pH: 7.4
Constituents: 100% PBS
Liquid
Monoclonal
Flow Cyt | ELISA | WB | |
---|---|---|---|
Human | Tested | Expected | Expected |
Rat | Predicted | Predicted | Predicted |
Chicken | Predicted | Predicted | Predicted |
Chimpanzee | Predicted | Predicted | Predicted |
Cow | Predicted | Predicted | Predicted |
Dog | Predicted | Predicted | Predicted |
Horse | Predicted | Predicted | Predicted |
Macaque monkey | Predicted | Predicted | Predicted |
Pig | Predicted | Predicted | Predicted |
Recombinant fragment - Human | Not recommended | Not recommended | Tested |
Recombinant full length protein | Not recommended | Tested | Not recommended |
Sheep | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Sheep, Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Macaque monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein, Recombinant fragment - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Sheep, Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Macaque monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Sheep, Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Macaque monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein | Dilution info - | Notes - |
Transmembrane serine/threonine kinase activin type-2 receptor forming an activin receptor complex with activin type-1 serine/threonine kinase receptors (ACVR1, ACVR1B or ACVR1c). Transduces the activin signal from the cell surface to the cytoplasm and is thus regulating many physiological and pathological processes including neuronal differentiation and neuronal survival, hair follicle development and cycling, FSH production by the pituitary gland, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. Activin is also thought to have a paracrine or autocrine role in follicular development in the ovary. Within the receptor complex, the type-2 receptors act as a primary activin receptors (binds activin-A/INHBA, activin-B/INHBB as well as inhibin-A/INHA-INHBA). The type-1 receptors like ACVR1B act as downstream transducers of activin signals. Activin binds to type-2 receptor at the plasma membrane and activates its serine-threonine kinase. The activated receptor type-2 then phosphorylates and activates the type-1 receptor. Once activated, the type-1 receptor binds and phosphorylates the SMAD proteins SMAD2 and SMAD3, on serine residues of the C-terminal tail. Soon after their association with the activin receptor and subsequent phosphorylation, SMAD2 and SMAD3 are released into the cytoplasm where they interact with the common partner SMAD4. This SMAD complex translocates into the nucleus where it mediates activin-induced transcription. Inhibitory SMAD7, which is recruited to ACVR1B through FKBP1A, can prevent the association of SMAD2 and SMAD3 with the activin receptor complex, thereby blocking the activin signal. Activin signal transduction is also antagonized by the binding to the receptor of inhibin-B via the IGSF1 inhibin coreceptor.
Activin receptor type-2B, Activin receptor type IIB, ACTR-IIB, ACVR2B
Mouse Monoclonal Activin Receptor Type IIB/ACVR2B antibody. Carrier free. Suitable for Flow Cyt, ELISA, WB and reacts with Human, Recombinant full length protein, Recombinant fragment - Human samples. Cited in 13 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ACVR2B aa 1-150.
Activin receptor type-2B, Activin receptor type IIB, ACTR-IIB, ACVR2B
IgG2a
Mouse
pH: 7.4
Constituents: 100% PBS
Liquid
Monoclonal
Yes
1C11
Tissue culture supernatant
kappa
Blue Ice
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This product was changed from ascites to tissue culture supernatant on 22/03/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
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If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
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Activin receptor type IIB participates in the regulation of muscle growth by controlling the activity of growth inhibitory proteins. The receptor forms complexes with type I receptor kinases initiating signal transduction events. ACVR2B also interfaces with the inhibin α subunit in the context of reproductive biology. Its involvement in these complexes and interactions underlines its significance in modulating physiological processes such as cell proliferation and differentiation.
Activin receptor type IIB (ACVR2B) also known as activin receptor type 2B or ACTR-IIB is a transmembrane serine/threonine kinase receptor. ACVR2B primarily binds activins though it can also interact with other ligands. This receptor weighs approximately 57 kDa. ACVR2B is widely expressed in various tissues including skeletal muscle heart and nervous tissue. The receptor plays an important role in transmitting signals from extracellular ligands to intracellular pathways influencing development and cellular functions.
Activin receptor type IIB plays a significant role in the TGF-beta signaling pathway and the myostatin pathway. These pathways regulate important cellular processes like muscular hypertrophy and tissue homeostasis. In these pathways ACVR2B interacts with proteins such as follistatin and myostatin. Follistatin acts as a binding protein modulating the activity of members in the TGF-beta superfamily including myostatin and activins highlighting ACVR2B's involvement in maintaining cellular equilibrium.
Activin receptor type IIB has links to muscular dystrophy and cancer. In muscular dystrophy the receptor's signaling affects muscle repair and regeneration primarily through its interaction with myostatin a known negative regulator of muscle growth. In cancer aberrant signaling through ACVR2B could contribute to tumor progression by affecting cellular proliferation and differentiation rates. Understanding ACVR2B's involvement in these diseases provides insights into potential therapeutic targets.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
All lanes: Western blot - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (AB76940)
Lane 1: IIB/ACVR2B transfected 293T cell lysate
Lane 2: Non-transfected 293T cell lysate
Predicted band size: 58 kDa
MW of GST tag alone: 26 kDa
This image was generated using the ascites version of the product.
All lanes: Western blot - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (AB76940) at 1/500 dilution
All lanes: immunogen (GST-tagged 100aa recombinant fragment) at 0.2 µg
All lanes: Goat anti-Mouse IgG (H&L) HRP-conjugated at 1/5000 dilution
Predicted band size: 58 kDa
Observed band size: 37 kDa
Detection limit for recombinant GST tagged ACVR2B is 0.1 ng/ml as a capture antibody.
Overlay histogram showing HeLa cells stained with ab76940 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76940, 0.5μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
This image was generated using the ascites version of the product.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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