Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (ab76940) is a mouse monoclonal antibody that is used to detect Activin Receptor Type IIB/ACVR2B in Western Blot, Flow Cytometry, ELISA. Suitable for Human, samples.
- Over 10 publications
- Trusted since 2009
Images
![Western blot - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (AB76940), expandable thumbnail](https://content.abcam.com/products/images/activin-receptor-type-iib-acvr2b-antibody-1c11-ab76940--western-blot-img163747.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (AB76940)")
![Western blot - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (AB76940), expandable thumbnail](https://content.abcam.com/products/images/activin-receptor-type-iib-acvr2b-antibody-1c11-ab76940--western-blot-img143343.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (AB76940)")
![ELISA - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (AB76940), expandable thumbnail](https://content.abcam.com/products/images/activin-receptor-type-iib-acvr2b-antibody-1c11-ab76940--elisa-img1514.jpg/_jcr_content/renditions/webp-475.webp "ELISA - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (AB76940)")
![Flow Cytometry - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (AB76940), expandable thumbnail](https://content.abcam.com/products/images/activin-receptor-type-iib-acvr2b-antibody-1c11-ab76940--flow-cytometry-img415067.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (AB76940)")
Publications
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- Proceedings of the National Academy of Sciences of the United States of America 120:e22108911202023State- and stimulus-specific dynamics of SMAD signaling determine fate decisions in individual cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStefan Bohn,Lorenz Hexemer,Zixin Huang,Laura Strohmaier,Sonja Lenhardt,Stefan Legewie,Alexander LoewerPubMed 36857347
- Cancers 15:2022Activin B and Activin C Have Opposing Effects on Prostate Cancer Progression and Cell Growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaren L Reader,Simon John-McHaffie,Sylvia Zellhuber-McMillan,Tim Jowett,David G Mottershead,Heather E Cunliffe,Elspeth J GoldPubMed 36612143
- Frontiers in cell and developmental biology 9:7741852022Myostatin Mutation Promotes Glycolysis by Increasing Phosphorylation of Phosphofructokinase Activation of PDE5A-cGMP-PKG in Cattle Heart.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingjuan Gu,Xinyu Zhou,Lin Zhu,Yajie Gao,Li Gao,Chunling Bai,Lei Yang,Guangpeng LiPubMed 35155444
- Frontiers in bioengineering and biotechnology 8:5342020Automated Hypothesis Generation to Identify Signals Relevant in the Development of Mammalian Cell and Tissue Bioprocesses, With Validation in a Retinal Culture System.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDerek Toms et. al.PubMed 32582664
- Cells 8:2019Expression of miRNAs from the Imprinted Locus Signals the Osteogenic Potential of Human Pluripotent Stem Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaetitia Barrault et. al.PubMed 31779280
- Journal of neuropathology and experimental neurology 78:130-1392019Myostatin Inhibition Using ActRIIB-mFc Does Not Produce Weight Gain or Strength in the Nebulin Conditional KO Mouse.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJennifer A Tinklenberg,Emily M Siebers,Margaret J Beatka,Brittany A Fickau,Samuel Ayres,Hui Meng,Lin Yang,Pippa Simpson,Henk L Granzier,Michael W LawlorPubMed 30597051
- Human molecular genetics 27:638-6482018Myostatin inhibition using mRK35 produces skeletal muscle growth and tubular aggregate formation in wild type and TgACTA1D286G nemaline myopathy mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJennifer A Tinklenberg,Emily M Siebers,Margaret J Beatka,Hui Meng,Lin Yang,Zizhao Zhang,Jacob A Ross,Julien Ochala,Carl Morris,Jane M Owens,Nigel G Laing,Kristen J Nowak,Michael W LawlorPubMed 29293963
- Endocrinology 158:1217-12302017Follistatin Targets Distinct Pathways To Promote Brown Adipocyte Characteristics in Brown and White Adipose Tissues.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRajan Singh,Melissa Braga,Srinivasa T Reddy,Se-Jin Lee,Meher Parveen,Victor Grijalva,Laurent Vergnes,Shehla PervinPubMed 28324027
- The American journal of pathology 186:1568-812016Treatment with ActRIIB-mFc Produces Myofiber Growth and Improves Lifespan in the Acta1 H40Y Murine Model of Nemaline Myopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJennifer Tinklenberg et. al.PubMed 27102768
- Skeletal muscle 6:142016Dystrophin-deficient dogs with reduced myostatin have unequal muscle growth and greater joint contractures.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoe N Kornegay,Daniel J Bogan,Janet R Bogan,Jennifer L Dow,Jiahui Wang,Zheng Fan,Naili Liu,Leigh C Warsing,Robert W Grange,Mihye Ahn,Cynthia J Balog-Alvarez,Steven W Cotten,Monte S Willis,Candice Brinkmeyer-Langford,Hongtu Zhu,Joe Palandra,Carl A Morris,Martin A Styner,Kathryn R WagnerPubMed 27047655
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human ACVR2B aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q13705
Reactivity data
Select an application| Flow Cyt | ELISA | WB | |
|---|---|---|---|
| Human | Tested | Expected | Expected |
| Rat | Predicted | Predicted | Predicted |
| Chicken | Predicted | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
| Horse | Predicted | Predicted | Predicted |
| Macaque monkey | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Recombinant fragment - Human | Not recommended | Not recommended | Tested |
| Recombinant full length protein | Not recommended | Tested | Not recommended |
| Sheep | Predicted | Predicted | Predicted |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Sheep, Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Macaque monkey | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein, Recombinant fragment - Human | Dilution info - | Notes - |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Sheep, Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Macaque monkey | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Sheep, Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Macaque monkey | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Transmembrane serine/threonine kinase activin type-2 receptor forming an activin receptor complex with activin type-1 serine/threonine kinase receptors (ACVR1, ACVR1B or ACVR1c). Transduces the activin signal from the cell surface to the cytoplasm and is thus regulating many physiological and pathological processes including neuronal differentiation and neuronal survival, hair follicle development and cycling, FSH production by the pituitary gland, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. Activin is also thought to have a paracrine or autocrine role in follicular development in the ovary. Within the receptor complex, the type-2 receptors act as a primary activin receptors (binds activin-A/INHBA, activin-B/INHBB as well as inhibin-A/INHA-INHBA). The type-1 receptors like ACVR1B act as downstream transducers of activin signals. Activin binds to type-2 receptor at the plasma membrane and activates its serine-threonine kinase. The activated receptor type-2 then phosphorylates and activates the type-1 receptor. Once activated, the type-1 receptor binds and phosphorylates the SMAD proteins SMAD2 and SMAD3, on serine residues of the C-terminal tail. Soon after their association with the activin receptor and subsequent phosphorylation, SMAD2 and SMAD3 are released into the cytoplasm where they interact with the common partner SMAD4. This SMAD complex translocates into the nucleus where it mediates activin-induced transcription. Inhibitory SMAD7, which is recruited to ACVR1B through FKBP1A, can prevent the association of SMAD2 and SMAD3 with the activin receptor complex, thereby blocking the activin signal. Activin signal transduction is also antagonized by the binding to the receptor of inhibin-B via the IGSF1 inhibin coreceptor.
Alternative names
Activin receptor type-2B, Activin receptor type IIB, ACTR-IIB, ACVR2B
Recommended products
Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (ab76940) is a mouse monoclonal antibody that is used to detect Activin Receptor Type IIB/ACVR2B in Western Blot, Flow Cytometry, ELISA. Suitable for Human, samples.
- Over 10 publications
- Trusted since 2009
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human ACVR2B aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q13705
- Carrier free
- Yes
- Clone number
- 1C11
- Purity
- Tissue culture supernatant
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (ab76940) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), ELISA in Human, samples.
What is the molecular weight of Activin Receptor Type IIB/ACVR2B?
Anti-Activin Receptor Type IIB/ACVR2B [1C11] (ab76940) specifically detects a band for Activin Receptor Type IIB/ACVR2B (UniProt: Q13705) at a molecular weight of 58kDa.
Trusted by the scientific community
Anti-Activin Receptor Type IIB/ACVR2B [1C11] (ab76940) was first used in a scientific publication in 2009 and has been cited over 10 times in peer-reviewed journals.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Activin receptor type IIB (ACVR2B) also known as activin receptor type 2B or ACTR-IIB is a transmembrane serine/threonine kinase receptor. ACVR2B primarily binds activins though it can also interact with other ligands. This receptor weighs approximately 57 kDa. ACVR2B is widely expressed in various tissues including skeletal muscle heart and nervous tissue. The receptor plays an important role in transmitting signals from extracellular ligands to intracellular pathways influencing development and cellular functions.
- Biological function summary
Activin receptor type IIB participates in the regulation of muscle growth by controlling the activity of growth inhibitory proteins. The receptor forms complexes with type I receptor kinases initiating signal transduction events. ACVR2B also interfaces with the inhibin α subunit in the context of reproductive biology. Its involvement in these complexes and interactions underlines its significance in modulating physiological processes such as cell proliferation and differentiation.
- Pathways
Activin receptor type IIB plays a significant role in the TGF-beta signaling pathway and the myostatin pathway. These pathways regulate important cellular processes like muscular hypertrophy and tissue homeostasis. In these pathways ACVR2B interacts with proteins such as follistatin and myostatin. Follistatin acts as a binding protein modulating the activity of members in the TGF-beta superfamily including myostatin and activins highlighting ACVR2B's involvement in maintaining cellular equilibrium.
- Associated diseases and disorders
Activin receptor type IIB has links to muscular dystrophy and cancer. In muscular dystrophy the receptor's signaling affects muscle repair and regeneration primarily through its interaction with myostatin a known negative regulator of muscle growth. In cancer aberrant signaling through ACVR2B could contribute to tumor progression by affecting cellular proliferation and differentiation rates. Understanding ACVR2B's involvement in these diseases provides insights into potential therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
![Western blot - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (ab76940), expandable thumbnail](https://content.abcam.com/products/images/activin-receptor-type-iib-acvr2b-antibody-1c11-ab76940--western-blot-img163747.jpg "Western blot - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (ab76940)")
Western blot - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (ab76940)
All lanes: Western blot - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (ab76940)
Lane 1: IIB/ACVR2B transfected 293T cell lysate
Lane 2: Non-transfected 293T cell lysate
Predicted band size: 58 kDa
![Western blot - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (ab76940), expandable thumbnail](https://content.abcam.com/products/images/activin-receptor-type-iib-acvr2b-antibody-1c11-ab76940--western-blot-img143343.jpg "Western blot - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (ab76940)")
Western blot - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (ab76940)
MW of GST tag alone: 26 kDa
This image was generated using the ascites version of the product.
All lanes: Western blot - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (ab76940) at 1/500 dilution
All lanes: immunogen (GST-tagged 100aa recombinant fragment) at 0.2 µg
SecondaryAll lanes: Goat anti-Mouse IgG (H&L) HRP-conjugated at 1/5000 dilution
Predicted band size: 58 kDa
Observed band size: 37 kDa
![ELISA - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (ab76940), expandable thumbnail](https://content.abcam.com/products/images/activin-receptor-type-iib-acvr2b-antibody-1c11-ab76940--elisa-img1514.jpg "ELISA - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (ab76940)")
ELISA - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (ab76940)
Detection limit for recombinant GST tagged ACVR2B is 0.1 ng/ml as a capture antibody.
![Flow Cytometry - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (ab76940), expandable thumbnail](https://content.abcam.com/products/images/activin-receptor-type-iib-acvr2b-antibody-1c11-ab76940--flow-cytometry-img415067.jpg "Flow Cytometry - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (ab76940)")
Flow Cytometry - Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (ab76940)
Overlay histogram showing HeLa cells stained with ab76940 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76940, 0.5μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control ab91361, 1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.This image was generated using the ascites version of the product.
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