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AB223716

Anti-ACTL7B antibody

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(1 Publication)

Rabbit Polyclonal ACTL7B antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ACTL7B aa 1-100.

View Alternative Names

Actin-like protein 7B, Actin-like-7-beta, ACTL7B

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTL7B antibody (AB223716)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTL7B antibody (AB223716)

Paraffin-embedded human testis tissue stained for ACTL7B using ab223716 at 1/50 dilution in immunohistochemical analysis.

Western blot - Anti-ACTL7B antibody (AB223716)
  • WB

Supplier Data

Western blot - Anti-ACTL7B antibody (AB223716)

All lanes:

Western blot - Anti-ACTL7B antibody (ab223716) at 1/100 dilution

Lane 1:

Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate (negative control)

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) overexpressing ACTL7B (co-expressed with a c-terminal myc-DDK tag), cell lysate

Predicted band size: 45 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ACTL7B aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q9Y614

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ACTL7B also known as Actin-Like Protein 7B belongs to the actin family of proteins. It shows structural properties similar to other actin proteins and exhibits a mass of approximately 45 kDa. The protein expresses specifically in the testis tissue suggesting a role that might be unique to this environment. Its expression pattern indicates that ACTL7B might play specialized roles in reproductive biology particularly within processes exclusive to male gamete cells.
Biological function summary

ACTL7B involves itself in the actin cytoskeleton organization impacting cell shape and motility. It is not known to be part of any multi-protein complex but it participates significantly in cellular processes that drive sperm development. ACTL7B contributes to the dynamics and restructuring of actin filaments which are critical in spermatogenesis. Its role in cytoskeleton reorganization implies its importance in numerous cellular movements and stability within the testis.

Pathways

ACTL7B interacts with mechanisms fundamental to cytoskeletal dynamics particularly within the spermatogenesis pathway. Related proteins in these pathways include other actin-binding proteins that modulate actin filament activity. The precise modulation of these filaments is important for the successful completion of sperm development as alterations in these pathway components may impact sperm functionality and fertility.

ACTL7B links to fertility-related conditions. Aberrations in its expression or function could contribute to male infertility due to disrupted spermatogenesis. Proteins like ACTL7A another actin-like protein sometimes exhibit compensation or redundancy complicating the pathway's influence on reproductive health when ACTL7B dysfunctions. Studies continue to investigate potential links between ACTL7B dynamics and specific fertility disorders emphasizing its relevance in reproductive medicine.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information ACTL7B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 9: PubMed39315548

2024

Pyruvate metabolism dictates fibroblast sensitivity to GLS1 inhibition during fibrogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Greg Contento,Jo-Anne Am Wilson,Brintha Selvarajah,Manuela Platé,Delphine Guillotin,Valle Morales,Marcello Trevisani,Vanessa Pitozzi,Katiuscia Bianchi,Rachel C Chambers
View all publications
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Product promise

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