Rabbit Polyclonal ACTL8 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ACTL8 aa 150 to C-terminus.
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- Thoracic cancer 11:526-5362020Actin-like protein 8 promotes cell proliferation, colony-formation, proangiogenesis, migration and invasion in lung adenocarcinoma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShanwu Ma et. al.PubMed 31962007
- Translational cancer research 8:1975-19842019Knockdown of actin-like 8 inhibits cell proliferation by regulating FOXM1, STMN1, PLK1, and BIRC5 in lung adenocarcinoma A549 cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShanwu Ma,Guangliang Qiang,Weipeng Shao,Chaoyang Liang,Xiaowei Wang,Deruo LiuPubMed 35116946
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ACTL8 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H568
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Antigen retrieval using heat mediated 10mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0) is suggested. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Alternative names
Actin-like protein 8, Cancer/testis antigen 57, CT57, ACTL8
Recommended products
Rabbit Polyclonal ACTL8 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ACTL8 aa 150 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ACTL8 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H568
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ACTL8 also known as Actin-Like 8 is a protein with a mass around 45 kDa. This protein is a member of the actin-related proteins that share structural similarities with conventional actin. ACTL8 is mainly expressed in the testis and to a lesser extent in the brain and other tissues. It plays a role in cellular processes that involve cytoskeletal dynamics.
- Biological function summary
Actin-Like 8 participates in intracellular transport and cell shape changes. It doesn't appear to form part of a well-defined complex but it may interact with other actin-related proteins and myosins in the testis. Its function is important in the development and maintenance of sperm tail structure suggesting a role in spermatogenesis.
- Pathways
ACTL8 is involved in actin cytoskeleton remodeling pathways. It connects to the Wnt signaling pathway which influences cell proliferation and differentiation. ACTL8 may interact with proteins like beta-catenin that bridge Wnt signaling and cytoskeletal organization which is important for cellular adhesion and migration processes.
- Associated diseases and disorders
Disturbances in ACTL8 expression have correlations with reproductive disorders such as non-obstructive azoospermia where sperm cells are not produced. It also has connections to neurological conditions given its expression in the brain although this is less well understood. Its interaction with proteins involved in cytoskeletal regulation such as other actins might influence disease states where cell movement and structure are affected.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-ACTL8 antibody (ab96756)
All lanes: Western blot - Anti-ACTL8 antibody (ab96756) at 1/1000 dilution
Lane 1: HepG2 whole cell lysate at 30 µg
Lane 2: Molt-4 whole cell lysate at 30 µg
Predicted band size: 41 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTL8 antibody (ab96756)
Immunohistochemical analysis of paraffin-embedded A549 xenograft , using ab96756 at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-ACTL8 antibody (ab96756)
ab96756 at 1/200 staining ACTL8 in methanol-fixed HeLa by Immunofluorescence, lower image is costained with Hoeschst 33342.
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