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AB184562

Anti-ACTL8 antibody [EPR13912]

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(2 Publications)

Rabbit Recombinant Monoclonal ACTL8 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

Actin-like protein 8, Cancer/testis antigen 57, CT57, ACTL8

4 Images
Flow Cytometry (Intracellular) - Anti-ACTL8 antibody [EPR13912] (AB184562)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ACTL8 antibody [EPR13912] (AB184562)

Intracellular flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling ACTL8 with ab184562 at 1/160 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Immunoprecipitation - Anti-ACTL8 antibody [EPR13912] (AB184562)
  • IP

Supplier Data

Immunoprecipitation - Anti-ACTL8 antibody [EPR13912] (AB184562)

Western blot analysis of immunoprecipitation pellet from HeLa cell lysate (lane 1) or PBS (lane 2) immunoprecipitated using ab184562 at 1/50 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG secondary antibody used at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-ACTL8 antibody [EPR13912] (ab184562)

Predicted band size: 41 kDa

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Western blot - Anti-ACTL8 antibody [EPR13912] (AB184562)
  • WB

Supplier Data

Western blot - Anti-ACTL8 antibody [EPR13912] (AB184562)

All lanes:

Western blot - Anti-ACTL8 antibody [EPR13912] (ab184562) at 1/2000 dilution

All lanes:

Human testis lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 41 kDa

true

Western blot - Anti-ACTL8 antibody [EPR13912] (AB184562)
  • WB

Supplier Data

Western blot - Anti-ACTL8 antibody [EPR13912] (AB184562)

All lanes:

Western blot - Anti-ACTL8 antibody [EPR13912] (ab184562) at 1/2000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 41 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13912

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40 - 1/60", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/160", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ACTL8 also known as Actin-Like 8 is a protein with a mass around 45 kDa. This protein is a member of the actin-related proteins that share structural similarities with conventional actin. ACTL8 is mainly expressed in the testis and to a lesser extent in the brain and other tissues. It plays a role in cellular processes that involve cytoskeletal dynamics.
Biological function summary

Actin-Like 8 participates in intracellular transport and cell shape changes. It doesn't appear to form part of a well-defined complex but it may interact with other actin-related proteins and myosins in the testis. Its function is important in the development and maintenance of sperm tail structure suggesting a role in spermatogenesis.

Pathways

ACTL8 is involved in actin cytoskeleton remodeling pathways. It connects to the Wnt signaling pathway which influences cell proliferation and differentiation. ACTL8 may interact with proteins like beta-catenin that bridge Wnt signaling and cytoskeletal organization which is important for cellular adhesion and migration processes.

Disturbances in ACTL8 expression have correlations with reproductive disorders such as non-obstructive azoospermia where sperm cells are not produced. It also has connections to neurological conditions given its expression in the brain although this is less well understood. Its interaction with proteins involved in cytoskeletal regulation such as other actins might influence disease states where cell movement and structure are affected.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information ACTL8
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

OncoTargets and therapy 14:2463-2473 PubMed33883901

2021

Actin-Like Protein 8 Promotes the Progression of Triple-Negative Breast Cancer via Activating PI3K/AKT/mTOR Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shaoxia Fan,Shen Yan,Yang Yang,Jian Shang,Min Hao

International journal of oncology 52:1209-1223 PubMed29532865

2018

CRISPR/Cas9-mediated ASXL1 mutations in U937 cells disrupt myeloid differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Jie Wu,Xin Zhao,Lauren G Banaszak,Fernanda Gutierrez-Rodrigues,Keyvan Keyvanfar,Shou-Guo Gao,Diego Quinones Raffo,Sachiko Kajigaya,Neal S Young
View all publications
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Product promise

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