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AB250763

Anti-ACTL8 antibody [EPR13912] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal ACTL8 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Actin-like protein 8, Cancer/testis antigen 57, CT57, ACTL8

4 Images
Flow Cytometry (Intracellular) - Anti-ACTL8 antibody [EPR13912] - BSA and Azide free (AB250763)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ACTL8 antibody [EPR13912] - BSA and Azide free (AB250763)

This data was developed using ab184562, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling ACTL8 with ab184562 at 1/160 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Immunoprecipitation - Anti-ACTL8 antibody [EPR13912] - BSA and Azide free (AB250763)
  • IP

Supplier Data

Immunoprecipitation - Anti-ACTL8 antibody [EPR13912] - BSA and Azide free (AB250763)

This data was developed using ab184562, the same antibody clone in a different buffer formulation.

Western blot analysis of immunoprecipitation pellet from HeLa cell lysate (lane 1) or PBS (lane 2) immunoprecipitated using ab184562 at 1/50 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG secondary antibody used at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-ACTL8 antibody [EPR13912] (<a href='/en-us/products/primary-antibodies/actl8-antibody-epr13912-ab184562'>ab184562</a>)

Predicted band size: 41 kDa

true

Western blot - Anti-ACTL8 antibody [EPR13912] - BSA and Azide free (AB250763)
  • WB

Supplier Data

Western blot - Anti-ACTL8 antibody [EPR13912] - BSA and Azide free (AB250763)

This data was developed using ab184562, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ACTL8 antibody [EPR13912] (<a href='/en-us/products/primary-antibodies/actl8-antibody-epr13912-ab184562'>ab184562</a>) at 1/2000 dilution

All lanes:

Human testis lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 41 kDa

true

Western blot - Anti-ACTL8 antibody [EPR13912] - BSA and Azide free (AB250763)
  • WB

Supplier Data

Western blot - Anti-ACTL8 antibody [EPR13912] - BSA and Azide free (AB250763)

This data was developed using ab184562, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ACTL8 antibody [EPR13912] (<a href='/en-us/products/primary-antibodies/actl8-antibody-epr13912-ab184562'>ab184562</a>) at 1/2000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 41 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13912

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

ab250763 is the carrier-free version of ab184562.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ACTL8 also known as Actin-Like 8 is a protein with a mass around 45 kDa. This protein is a member of the actin-related proteins that share structural similarities with conventional actin. ACTL8 is mainly expressed in the testis and to a lesser extent in the brain and other tissues. It plays a role in cellular processes that involve cytoskeletal dynamics.
Biological function summary

Actin-Like 8 participates in intracellular transport and cell shape changes. It doesn't appear to form part of a well-defined complex but it may interact with other actin-related proteins and myosins in the testis. Its function is important in the development and maintenance of sperm tail structure suggesting a role in spermatogenesis.

Pathways

ACTL8 is involved in actin cytoskeleton remodeling pathways. It connects to the Wnt signaling pathway which influences cell proliferation and differentiation. ACTL8 may interact with proteins like beta-catenin that bridge Wnt signaling and cytoskeletal organization which is important for cellular adhesion and migration processes.

Disturbances in ACTL8 expression have correlations with reproductive disorders such as non-obstructive azoospermia where sperm cells are not produced. It also has connections to neurological conditions given its expression in the brain although this is less well understood. Its interaction with proteins involved in cytoskeletal regulation such as other actins might influence disease states where cell movement and structure are affected.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information ACTL8
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

World journal of gastrointestinal oncology 16:4716-4727 PubMed39678812

2024

Enhancing the radiosensitivity of colorectal cancer cells by reducing spermine synthase through promoting autophagy and DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Bin Guo,Yue-Ming Wu,Zhi-Zhao Lin
View all publications
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Product promise

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