Rabbit Recombinant Monoclonal ACTN3 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 5 publications.
Images
![Flow Cytometry (Intracellular) - Anti-ACTN3 antibody [EP2531Y] - Loading Control (AB68204), expandable thumbnail](https://content.abcam.com/products/images/actn3-antibody-ep2531y-loading-control-ab68204--flow-cytometry-intracellular-img26469.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-ACTN3 antibody [EP2531Y] - Loading Control (AB68204)")
![Western blot - Anti-ACTN3 antibody [EP2531Y] - Loading Control (AB68204), expandable thumbnail](https://content.abcam.com/products/images/actn3-antibody-ep2531y-loading-control-ab68204--western-blot-img11404.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ACTN3 antibody [EP2531Y] - Loading Control (AB68204)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTN3 antibody [EP2531Y] - Loading Control (AB68204), expandable thumbnail](https://content.abcam.com/products/images/actn3-antibody-ep2531y-loading-control-ab68204--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107739.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTN3 antibody [EP2531Y] - Loading Control (AB68204)")
![Western blot - Anti-ACTN3 antibody [EP2531Y] - Loading Control (AB68204), expandable thumbnail](https://content.abcam.com/products/images/actn3-antibody-ep2531y-loading-control-ab68204--western-blot-img8295.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ACTN3 antibody [EP2531Y] - Loading Control (AB68204)")
![Western blot - Anti-ACTN3 antibody [EP2531Y] - Loading Control (AB68204), expandable thumbnail](https://content.abcam.com/products/images/actn3-antibody-ep2531y-loading-control-ab68204--western-blot-img29950.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ACTN3 antibody [EP2531Y] - Loading Control (AB68204)")
Publications
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- Frontiers in genetics 14:12160662023Transcriptomic analysis of paired healthy human skeletal muscles to identify modulators of disease severity in DMD.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShirley Nieves-Rodriguez,Florian Barthélémy,Jeremy D Woods,Emilie D Douine,Richard T Wang,Deirdre D Scripture-Adams,Kevin N Chesmore,Francesca Galasso,M Carrie Miceli,Stanley F NelsonPubMed 37576554
- Nature communications 12:12942021Maintenance of type 2 glycolytic myofibers with age by Mib1-Actn3 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJi-Yun Seo,Jong-Seol Kang,Ye Lynne Kim,Young-Woo Jo,Ji-Hoon Kim,Sang-Hyeon Hann,Jieon Park,Inkuk Park,Hyerim Park,Kyusang Yoo,Joonwoo Rhee,Jung-Wee Park,Yong Chan Ha,Young-Yun KongPubMed 33637766
- American journal of human genetics 108:446-4572021Loss of α-actinin-3 during human evolution provides superior cold resilience and muscle heat generation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVictoria L Wyckelsma,Tomas Venckunas,Peter J Houweling,Maja Schlittler,Volker M Lauschke,Chrystal F Tiong,Harrison D Wood,Niklas Ivarsson,Henrikas Paulauskas,Nerijus Eimantas,Daniel C Andersson,Kathryn N North,Marius Brazaitis,Håkan WesterbladPubMed 33600773
- Acta neuropathologica 137:501-5192019ACTN2 mutations cause "Multiple structured Core Disease" (MsCD).Applications:Unspecified applicationReactive species:Unspecified reactive speciesXavière Lornage et. al.PubMed 30701273
- Histochemistry and cell biology 148:545-5552017Human skeletal muscle type 1 fibre distribution and response of stress-sensing proteins along the titin molecule after submaximal exhaustive exercise.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSatu O A Koskinen et. al.PubMed 28712031
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Expected |
| Mouse | Expected | Not recommended | Tested | Expected |
| Rat | Expected | Not recommended | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/5000 - 1/10000 | Notes - |
Species Human | Dilution info 1/5000 - 1/10000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/30 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
Alternative names
Alpha-actinin-3, Alpha-actinin skeletal muscle isoform 3, F-actin cross-linking protein, ACTN3
Recommended products
Rabbit Recombinant Monoclonal ACTN3 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 5 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP2531Y
- Purification technique
- Affinity purification Protein A
- Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The ACTN3 protein also known as alpha-actinin-3 has a mechanical role in muscle function. This protein which weighs around 103 kDa is a structural component of the Z-disc in skeletal muscle fibers. It crosslinks actin filaments and stabilizes the sarcomere during muscle contraction and force transmission. ACTN3 is expressed in fast-twitch muscle fibers which are essential for rapid and forceful contractions.
- Biological function summary
ACTN3 influences muscle performance and fiber type composition. As part of a complex it interacts with other proteins in the Z-disc helping maintain muscle integrity and function. The presence or absence of ACTN3 affects muscle strength power and endurance. Its expression level varies among individuals impacting their athletic performance capabilities.
- Pathways
The ACTN3 protein plays a role in cellular pathways linked to muscle contraction and energy metabolism. It is involved in the regulation of the glycolysis pathway a critical energy source for fast-twitch muscles. ACTN2 a closely related protein can compensate for some functions of ACTN3 showing their interaction within these pathways. This functional relationship is important for maintaining muscle function under different metabolic conditions.
- Associated diseases and disorders
ACTN3 relates to muscular dystrophies and athletic performance capabilities. Individuals lacking functional ACTN3 due to a common null polymorphism may show differences in muscle power output impacting conditions like sarcopenia characterized by age-related muscle decline. The protein also associates with dystrophin in muscular disorders like Duchenne Muscular Dystrophy highlighting its importance in maintaining skeletal muscle health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Flow Cytometry (Intracellular) - Anti-ACTN3 antibody [EP2531Y] - Loading Control (ab68204), expandable thumbnail](https://content.abcam.com/products/images/actn3-antibody-ep2531y-loading-control-ab68204--flow-cytometry-intracellular-img26469.jpg "Flow Cytometry (Intracellular) - Anti-ACTN3 antibody [EP2531Y] - Loading Control (ab68204)")
Flow Cytometry (Intracellular) - Anti-ACTN3 antibody [EP2531Y] - Loading Control (ab68204)
ACTN3 Flow Cytometry (Intracellular) staining using rabbit Anti-ACTN3 antibody
Intracellular Flow Cytometry analysis of L6 (Rat skeletal muscle myoblast) cells labeling ACTN3 with purified ab68204 at 1/30 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
![Western blot - Anti-ACTN3 antibody [EP2531Y] - Loading Control (ab68204), expandable thumbnail](https://content.abcam.com/products/images/actn3-antibody-ep2531y-loading-control-ab68204--western-blot-img11404.jpg "Western blot - Anti-ACTN3 antibody [EP2531Y] - Loading Control (ab68204)")
Western blot - Anti-ACTN3 antibody [EP2531Y] - Loading Control (ab68204)
ACTN3 Western blot staining using rabbit Anti-ACTN3 antibody
All lanes: Western blot - Anti-ACTN3 antibody [EP2531Y] - Loading Control (ab68204) at 1/20000 dilution
Lane 1: Mouse skeletal muscle lysates at 15 µg
Lane 2: Rat skeletal muscle lysates at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 103 kDa
Observed band size: 100 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTN3 antibody [EP2531Y] - Loading Control (ab68204), expandable thumbnail](https://content.abcam.com/products/images/actn3-antibody-ep2531y-loading-control-ab68204--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107739.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTN3 antibody [EP2531Y] - Loading Control (ab68204)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTN3 antibody [EP2531Y] - Loading Control (ab68204)
ACTN3 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-ACTN3 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue sections labeling ACTN3 with purified ab68204 at 1/250 dilution (0.97 μg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
![Western blot - Anti-ACTN3 antibody [EP2531Y] - Loading Control (ab68204), expandable thumbnail](https://content.abcam.com/products/images/actn3-antibody-ep2531y-loading-control-ab68204--western-blot-img8295.jpg "Western blot - Anti-ACTN3 antibody [EP2531Y] - Loading Control (ab68204)")
Western blot - Anti-ACTN3 antibody [EP2531Y] - Loading Control (ab68204)
ACTN3 Western blot staining of Human skeletal muscle lysates using rabbit Anti-ACTN3 antibody
All lanes: Western blot - Anti-ACTN3 antibody [EP2531Y] - Loading Control (ab68204) at 1/5000 dilution
All lanes: Human skeletal muscle lysates at 15 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 103 kDa
Observed band size: 100 kDa
![Western blot - Anti-ACTN3 antibody [EP2531Y] - Loading Control (ab68204), expandable thumbnail](https://content.abcam.com/products/images/actn3-antibody-ep2531y-loading-control-ab68204--western-blot-img29950.jpg "Western blot - Anti-ACTN3 antibody [EP2531Y] - Loading Control (ab68204)")
Western blot - Anti-ACTN3 antibody [EP2531Y] - Loading Control (ab68204)
ACTN3 Western blot staining of human skeletal muscle lysate using rabbit Anti-ACTN3 antibody
All lanes: Western blot - Anti-ACTN3 antibody [EP2531Y] - Loading Control (ab68204) at 1/10000 dilution
All lanes: human skeletal muscle lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 103 kDa
Observed band size: 103 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTN3 antibody [EP2531Y] - Loading Control (ab68204), expandable thumbnail](https://content.abcam.com/products/images/actn3-antibody-ep2531y-loading-control-ab68204--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img66525.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTN3 antibody [EP2531Y] - Loading Control (ab68204)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTN3 antibody [EP2531Y] - Loading Control (ab68204)
ACTN3 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-ACTN3 antibody
Immunohistochemical analysis of paraffin-embedded human muscle tissue using unpurified ab68204 at 1/100-1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTN3 antibody [EP2531Y] - Loading Control (ab68204), expandable thumbnail](https://content.abcam.com/products/images/actn3-antibody-ep2531y-loading-control-ab68204--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img66525.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTN3 antibody [EP2531Y] - Loading Control (ab68204)")
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