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AB68204

Anti-ACTN3 antibody [EP2531Y] - Loading Control

  • BOND RX™ Validated
  • RabMAb
  • Recombinant
  • Lab Essentials
  • What is this?

5

(4 Reviews)

|

(8 Publications)

Rabbit Recombinant Monoclonal ACTN3 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 8 publications.

View Alternative Names

Alpha-actinin-3, Alpha-actinin skeletal muscle isoform 3, F-actin cross-linking protein, ACTN3

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTN3 antibody [EP2531Y] - Loading Control (AB68204)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTN3 antibody [EP2531Y] - Loading Control (AB68204)

Immunohistochemical analysis of paraffin-embedded human muscle tissue using unpurified ab68204 at 1/100-1/250 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTN3 antibody [EP2531Y] - Loading Control (AB68204)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTN3 antibody [EP2531Y] - Loading Control (AB68204)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue sections labeling ACTN3 with purified ab68204 at 1/250 dilution (0.97 μg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Flow Cytometry (Intracellular) - Anti-ACTN3 antibody [EP2531Y] - Loading Control (AB68204)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-ACTN3 antibody [EP2531Y] - Loading Control (AB68204)

Intracellular Flow Cytometry analysis of L6 (Rat skeletal muscle myoblast) cells labeling ACTN3 with purified ab68204 at 1/30 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Western blot - Anti-ACTN3 antibody [EP2531Y] - Loading Control (AB68204)
  • WB

Unknown

Western blot - Anti-ACTN3 antibody [EP2531Y] - Loading Control (AB68204)

All lanes:

Western blot - Anti-ACTN3 antibody [EP2531Y] - Loading Control (ab68204) at 1/10000 dilution

All lanes:

human skeletal muscle lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 103 kDa

Observed band size: 103 kDa

false

Western blot - Anti-ACTN3 antibody [EP2531Y] - Loading Control (AB68204)
  • WB

Lab

Western blot - Anti-ACTN3 antibody [EP2531Y] - Loading Control (AB68204)

All lanes:

Western blot - Anti-ACTN3 antibody [EP2531Y] - Loading Control (ab68204) at 1/5000 dilution

All lanes:

Human skeletal muscle lysates at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 103 kDa

Observed band size: 100 kDa

false

Western blot - Anti-ACTN3 antibody [EP2531Y] - Loading Control (AB68204)
  • WB

Lab

Western blot - Anti-ACTN3 antibody [EP2531Y] - Loading Control (AB68204)

All lanes:

Western blot - Anti-ACTN3 antibody [EP2531Y] - Loading Control (ab68204) at 1/20000 dilution

Lane 1:

Mouse skeletal muscle lysates at 15 µg

Lane 2:

Rat skeletal muscle lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 103 kDa

Observed band size: 100 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2531Y

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

AB210420

Alexa Fluor® 488 Anti-ACTN3 antibody [EP2531Y] - Cytoskeleton Marker

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Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-ACTN3 antibody [EP2531Y] - Cytoskeleton Marker (AB210420)

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Primary Antibodies

AB113234

Anti-ACTN3 antibody - Loading Control

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTN3 antibody - Loading Control (AB113234)

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Complementary Products

Secondary Antibodies

AB175653

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed (AB175653)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

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Primary Antibodies

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Anti-Argonaute-2 antibody [EPR10411]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10411] (AB186733)

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Primary Antibodies

AB37484

Anti-Myosin heavy chain antibody [A4.1025]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin heavy chain antibody [A4.1025] (AB37484)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/30", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ACTN3 protein also known as alpha-actinin-3 has a mechanical role in muscle function. This protein which weighs around 103 kDa is a structural component of the Z-disc in skeletal muscle fibers. It crosslinks actin filaments and stabilizes the sarcomere during muscle contraction and force transmission. ACTN3 is expressed in fast-twitch muscle fibers which are essential for rapid and forceful contractions.
Biological function summary

ACTN3 influences muscle performance and fiber type composition. As part of a complex it interacts with other proteins in the Z-disc helping maintain muscle integrity and function. The presence or absence of ACTN3 affects muscle strength power and endurance. Its expression level varies among individuals impacting their athletic performance capabilities.

Pathways

The ACTN3 protein plays a role in cellular pathways linked to muscle contraction and energy metabolism. It is involved in the regulation of the glycolysis pathway a critical energy source for fast-twitch muscles. ACTN2 a closely related protein can compensate for some functions of ACTN3 showing their interaction within these pathways. This functional relationship is important for maintaining muscle function under different metabolic conditions.

ACTN3 relates to muscular dystrophies and athletic performance capabilities. Individuals lacking functional ACTN3 due to a common null polymorphism may show differences in muscle power output impacting conditions like sarcopenia characterized by age-related muscle decline. The protein also associates with dystrophin in muscular disorders like Duchenne Muscular Dystrophy highlighting its importance in maintaining skeletal muscle health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
See full target information ACTN3
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadw1059 PubMed40864710

2025

genotype influences androgen response in developing murine skeletal muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Kelly N Roeszler,Michael See,Lyra R Meehan,Giscard Lima,Alexander Kolliari-Turner,Sarah E Alexander,Shanie Landen,Harrison D Wood,Chrystal F Tiong,Weiyi Chen,Tomris Mustafa,Peter J Houweling,Nir Eynon,Severine Lamon,Yannis Pitsiladis,David J Handelsman,Fernando J Rossello,Mirana Ramialison,Kathryn N North,Jane T Seto

Frontiers in genetics 14:1216066 PubMed37576554

2023

Transcriptomic analysis of paired healthy human skeletal muscles to identify modulators of disease severity in DMD.

Applications

Unspecified application

Species

Unspecified reactive species

Shirley Nieves-Rodriguez,Florian Barthélémy,Jeremy D Woods,Emilie D Douine,Richard T Wang,Deirdre D Scripture-Adams,Kevin N Chesmore,Francesca Galasso,M Carrie Miceli,Stanley F Nelson

International journal of molecular sciences 23: PubMed35563042

2022

Identification of Potential Muscle Biomarkers in McArdle Disease: Insights from Muscle Proteome Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Inés García-Consuegra,Sara Asensio-Peña,Rocío Garrido-Moraga,Tomàs Pinós,Cristina Domínguez-González,Alfredo Santalla,Gisela Nogales-Gadea,Pablo Serrano-Lorenzo,Antoni L Andreu,Joaquín Arenas,José L Zugaza,Alejandro Lucia,Miguel A Martín

Science advances 7: PubMed34215586

2021

genotype influences skeletal muscle mass regulation and response to dexamethasone.

Applications

Unspecified application

Species

Unspecified reactive species

Jane T Seto,Kelly N Roeszler,Lyra R Meehan,Harrison D Wood,Chrystal Tiong,Lucinda Bek,Siaw F Lee,Manan Shah,Kate G R Quinlan,Paul Gregorevic,Peter J Houweling,Kathryn N North

Nature communications 12:1294 PubMed33637766

2021

Maintenance of type 2 glycolytic myofibers with age by Mib1-Actn3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Yun Seo,Jong-Seol Kang,Ye Lynne Kim,Young-Woo Jo,Ji-Hoon Kim,Sang-Hyeon Hann,Jieon Park,Inkuk Park,Hyerim Park,Kyusang Yoo,Joonwoo Rhee,Jung-Wee Park,Yong Chan Ha,Young-Yun Kong

American journal of human genetics 108:446-457 PubMed33600773

2021

Loss of α-actinin-3 during human evolution provides superior cold resilience and muscle heat generation.

Applications

Unspecified application

Species

Unspecified reactive species

Victoria L Wyckelsma,Tomas Venckunas,Peter J Houweling,Maja Schlittler,Volker M Lauschke,Chrystal F Tiong,Harrison D Wood,Niklas Ivarsson,Henrikas Paulauskas,Nerijus Eimantas,Daniel C Andersson,Kathryn N North,Marius Brazaitis,Håkan Westerblad

Acta neuropathologica 137:501-519 PubMed30701273

2019

ACTN2 mutations cause "Multiple structured Core Disease" (MsCD).

Applications

Unspecified application

Species

Unspecified reactive species

Xavière Lornage,Norma B Romero,Claire A Grosgogeat,Edoardo Malfatti,Sandra Donkervoort,Michael M Marchetti,Sarah B Neuhaus,A Reghan Foley,Clémence Labasse,Raphaël Schneider,Robert Y Carlier,Katherine R Chao,Livija Medne,Jean-François Deleuze,David Orlikowski,Carsten G Bönnemann,Vandana A Gupta,Michel Fardeau,Johann Böhm,Jocelyn Laporte

Histochemistry and cell biology 148:545-555 PubMed28712031

2017

Human skeletal muscle type 1 fibre distribution and response of stress-sensing proteins along the titin molecule after submaximal exhaustive exercise.

Applications

Unspecified application

Species

Unspecified reactive species

Satu O A Koskinen,Heikki Kyröläinen,Riina Flink,Harri P Selänne,Sheila S Gagnon,Juha P Ahtiainen,Bradley C Nindl,Maarit Lehti
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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