AB113234

Anti-ACTN3 antibody - Loading Control

Lab Essentials
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(1 Publication)

Rabbit Polyclonal ACTN3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ACTN3 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Alpha-actinin-3, Alpha-actinin skeletal muscle isoform 3, F-actin cross-linking protein, ACTN3

2 Images

Unknown

Western blot - Anti-ACTN3 antibody - Loading Control (AB113234)

All lanes:

Western blot - Anti-ACTN3 antibody - Loading Control (ab113234) at 1/500 dilution

Lane 1:

Extracts from HT-29 cells

Lane 2:

Extracts from HT-29 cells with treated with synthetic peptide

Predicted band size: 103 kDa

false

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTN3 antibody - Loading Control (AB113234)

ab113234 at 10 μg/ml staining ACTN3 in formalin-fixed, paraffin-embedded Human skeletal muscle tissue.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human ACTN3 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q08043

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 48% PBS, 0.88% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ACTN3 protein also known as alpha-actinin-3 has a mechanical role in muscle function. This protein which weighs around 103 kDa is a structural component of the Z-disc in skeletal muscle fibers. It crosslinks actin filaments and stabilizes the sarcomere during muscle contraction and force transmission. ACTN3 is expressed in fast-twitch muscle fibers which are essential for rapid and forceful contractions.

Biological function summary

ACTN3 influences muscle performance and fiber type composition. As part of a complex it interacts with other proteins in the Z-disc helping maintain muscle integrity and function. The presence or absence of ACTN3 affects muscle strength power and endurance. Its expression level varies among individuals impacting their athletic performance capabilities.

Pathways

The ACTN3 protein plays a role in cellular pathways linked to muscle contraction and energy metabolism. It is involved in the regulation of the glycolysis pathway a critical energy source for fast-twitch muscles. ACTN2 a closely related protein can compensate for some functions of ACTN3 showing their interaction within these pathways. This functional relationship is important for maintaining muscle function under different metabolic conditions.

ACTN3 relates to muscular dystrophies and athletic performance capabilities. Individuals lacking functional ACTN3 due to a common null polymorphism may show differences in muscle power output impacting conditions like sarcopenia characterized by age-related muscle decline. The protein also associates with dystrophin in muscular disorders like Duchenne Muscular Dystrophy highlighting its importance in maintaining skeletal muscle health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

See full target information ACTN3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 11:e0160384 PubMed27611087

2016

Novel Metabolic Abnormalities in the Tricarboxylic Acid Cycle in Peripheral Cells From Huntington's Disease Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Nima N Naseri,Joseph Bonica,Hui Xu,Larry C Park,Jamshid Arjomand,Zhengming Chen,Gary E Gibson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ACTN3 antibody - Loading Control

Western blot - Anti-ACTN3 antibody - Loading Control (AB113234)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTN3 antibody - Loading Control (AB113234)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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