Skip to main content

Rabbit Recombinant Monoclonal ACTR1B antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

Be the first to review this product! Submit a review

Images

Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (AB203833), expandable thumbnail
  • Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (AB203833), expandable thumbnail
  • Immunoprecipitation - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (AB203833), expandable thumbnail
  • Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (AB203833), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/40
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Target data

Function

Component of a multi-subunit complex involved in microtubule based vesicle motility. It is associated with the centrosome.

Additional Targets

ACTR1A

Alternative names

CTRN2, ACTR1B, Beta-centractin, Actin-related protein 1B, ARP1B

Recommended products

Rabbit Recombinant Monoclonal ACTR1B antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR16968(B)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ACTR1A and ACTR1B also known as Actin-related protein 1A and 1B play roles in cellular processes by interacting with the cytoskeleton. While ACTR1A has a mass of approximately 42 kDa ACTR1B weighs around 48 kDa. Both proteins have widespread expression in multiple tissue types where they influence cellular structure and dynamics. They are especially important in cells undergoing rapid movement or growth.

Biological function summary

These proteins contribute to cytoskeletal remodeling processes essential for cellular functions. They act as components of the dynactin complex which associates with microtubules to regulate intracellular transport. Their activity is important for cellular activities that involve cytoplasmic organization including endocytosis and the distribution of organelles. The effective functioning of ACTR1A and ACTR1B supports cellular responses to external stimuli and environmental changes.

Pathways

ACTR1A and ACTR1B engage in the regulation of the MAPK and Actin cytoskeleton pathways. Within these pathways they work with proteins such as DYNLL1 and DCTN1 to facilitate these cellular processes. The ACTR1A and ACTR1B proteins ensure proper cellular responses to signaling cues that can impact cell fate and behavior through these pathways.

Associated diseases and disorders

ACTR1A and ACTR1B have connections to neurodegenerative diseases like Alzheimer's and certain types of cancer. Related to Alzheimer's changes in the function or expression of ACTR1A and ACTR1B can influence pathways involved in neuronal damage and synapse loss. For cancer their role in cell motility and division is significant with alterations linked to metastasis and tumor progression. Their interaction with proteins like tau in Alzheimer's and oncogenes in cancer reflects the complexity of their involvement in disease contexts.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (ab203833), expandable thumbnail

    Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (ab203833)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (ab203833) at 1/1000 dilution

    Lane 1: Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 10 µg

    Lane 2: MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate at 10 µg

    Lane 3: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 122 kDa, 42 kDa, 53 kDa

    Observed band size: 42 kDa

    Exposure time: 3min

  • Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (ab203833), expandable thumbnail

    Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (ab203833)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (ab203833) at 1/1000 dilution

    Lane 1: Human fetal brain tissue lysate at 10 µg

    Lane 2: Human fetal kidney tissue lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 42 kDa

    Observed band size: 42 kDa

    Exposure time: 3min

  • Immunoprecipitation - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (ab203833), expandable thumbnail

    Immunoprecipitation - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (ab203833)

    ACTR1B + ACTR1A was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate with ab203833 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab203833 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1500.

    Lane 1: Jurkat whole cell lysate 10ug (Input). Lane 2: ab203833 IP in Jurkat whole cell lysate. Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab203833 in Jurkat whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    All lanes: Immunoprecipitation - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (ab203833)

  • Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (ab203833), expandable thumbnail

    Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (ab203833)

    Lane 1: Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (ab203833) at 1/1000 dilution

    Lane 1: Western blot - Anti-ACTR1B antibody [EPR16969] (Anti-ACTR1B antibody [EPR16969] ab203835) at 1/1000 dilution

    All lanes: Full length Human ACTR1A recombination protein at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 42 kDa

    Observed band size: 42 kDa

    Exposure time: 3s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com