AB203833

Anti-ACTR1B + ACTR1A antibody [EPR16968(B)]

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal ACTR1B antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

CTRN2, ACTR1B, Beta-centractin, Actin-related protein 1B, ARP1B, CTRN1, ACTR1A, Alpha-centractin, Centractin, ARP1, Actin-RPV, Centrosome-associated actin homolog

4 Images

Supplier Data

Immunoprecipitation - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (AB203833)

ACTR1B + ACTR1A was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate with ab203833 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab203833 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1500.

Lane 1 : Jurkat whole cell lysate 10ug (Input). Lane 2 : ab203833 IP in Jurkat whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab203833 in Jurkat whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (ab203833)

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Supplier Data

Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (AB203833)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (ab203833) at 1/1000 dilution

Lane 1:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 10 µg

Lane 2:

MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate at 10 µg

Lane 3:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 122 kDa,42 kDa,53 kDa

Observed band size: 42 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (AB203833)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (ab203833) at 1/1000 dilution

Lane 1:

Human fetal brain tissue lysate at 10 µg

Lane 2:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (AB203833)

Lane 1:

Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (ab203833) at 1/1000 dilution

Lane 1:

Western blot - Anti-ACTR1B antibody [EPR16969] (<a href='/en-us/products/primary-antibodies/actr1b-antibody-epr16969-ab203835'>ab203835</a>) at 1/1000 dilution

All lanes:

Full length Human ACTR1A recombination protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

false

Exposure time: 3s

Carrier free

Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16968(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ACTR1A and ACTR1B also known as Actin-related protein 1A and 1B play roles in cellular processes by interacting with the cytoskeleton. While ACTR1A has a mass of approximately 42 kDa ACTR1B weighs around 48 kDa. Both proteins have widespread expression in multiple tissue types where they influence cellular structure and dynamics. They are especially important in cells undergoing rapid movement or growth.

Biological function summary

These proteins contribute to cytoskeletal remodeling processes essential for cellular functions. They act as components of the dynactin complex which associates with microtubules to regulate intracellular transport. Their activity is important for cellular activities that involve cytoplasmic organization including endocytosis and the distribution of organelles. The effective functioning of ACTR1A and ACTR1B supports cellular responses to external stimuli and environmental changes.

Pathways

ACTR1A and ACTR1B engage in the regulation of the MAPK and Actin cytoskeleton pathways. Within these pathways they work with proteins such as DYNLL1 and DCTN1 to facilitate these cellular processes. The ACTR1A and ACTR1B proteins ensure proper cellular responses to signaling cues that can impact cell fate and behavior through these pathways.

ACTR1A and ACTR1B have connections to neurodegenerative diseases like Alzheimer's and certain types of cancer. Related to Alzheimer's changes in the function or expression of ACTR1A and ACTR1B can influence pathways involved in neuronal damage and synapse loss. For cancer their role in cell motility and division is significant with alterations linked to metastasis and tumor progression. Their interaction with proteins like tau in Alzheimer's and oncogenes in cancer reflects the complexity of their involvement in disease contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of a multi-subunit complex involved in microtubule based vesicle motility. It is associated with the centrosome.

See full target information ACTR1B

Additional targets

ACTR1A

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 70:103039 PubMed38241838

2024

SPTBN2 suppresses ferroptosis in NSCLC cells by facilitating SLC7A11 membrane trafficking and localization.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Deng,Xu Lin,Jiajia Qin,Qi Li,Yingqiong Zhang,Qingyi Zhang,Cong Ji,Shuying Shen,Yangling Li,Bo Zhang,Nengming Lin

Molecular & cellular proteomics : MCP 18:1732-1744 PubMed31221720

2019

Cross-linking Proteomics Indicates Effects of Simvastatin on the TLR2 Interactome and Reveals ACTR1A as a Novel Regulator of the TLR2 Signal Cascade.

Applications

Unspecified application

Species

Unspecified reactive species

Abu Hena Mostafa Kamal,Jim J Aloor,Michael B Fessler,Saiful M Chowdhury

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ACTR1B + ACTR1A antibody [EPR16968(B)]

Immunoprecipitation - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (AB203833)

Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (AB203833)

Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (AB203833)

Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (AB203833)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (2)

Product promise