Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] - BSA and Azide free (AB251404)

This data was developed using ab203833, the same antibody clone in a different buffer formulation.

ACTR1B + ACTR1A was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate with ab203833 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab203833 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1500.

Lane 1 : Jurkat whole cell lysate 10ug (Input). Lane 2 : ab203833 IP in Jurkat whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab203833 in Jurkat whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (<a href='/en-us/products/primary-antibodies/actr1b-actr1a-antibody-epr16968b-ab203833'>ab203833</a>)

false

• WB

Supplier Data

Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] - BSA and Azide free (AB251404)

This data was developed using ab203833, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (<a href='/en-us/products/primary-antibodies/actr1b-actr1a-antibody-epr16968b-ab203833'>ab203833</a>) at 1/1000 dilution

Lane 1:

Human fetal brain tissue lysate at 10 µg

Lane 2:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] - BSA and Azide free (AB251404)

This data was developed using ab203833, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (<a href='/en-us/products/primary-antibodies/actr1b-actr1a-antibody-epr16968b-ab203833'>ab203833</a>) at 1/1000 dilution

Lane 1:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 10 µg

Lane 2:

MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate at 10 µg

Lane 3:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 122 kDa,42 kDa,53 kDa

Observed band size: 42 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] - BSA and Azide free (AB251404)

This data was developed using ab203833, the same antibody clone in a different buffer formulation.

Lane 1:

Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (<a href='/en-us/products/primary-antibodies/actr1b-actr1a-antibody-epr16968b-ab203833'>ab203833</a>) at 1/1000 dilution

Lane 1:

Western blot - Anti-ACTR1B antibody [EPR16969] (<a href='/en-us/products/primary-antibodies/actr1b-antibody-epr16969-ab203835'>ab203835</a>) at 1/1000 dilution

All lanes:

Full length Human ACTR1A recombination protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

false

Exposure time: 3s