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Rabbit Recombinant Monoclonal ACTR1B antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples.

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Images

Western blot - Anti-ACTR1B antibody [EPR16969] (AB203835), expandable thumbnail
  • Western blot - Anti-ACTR1B antibody [EPR16969] (AB203835), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTR1B antibody [EPR16969] (AB203835), expandable thumbnail
  • Western blot - Anti-ACTR1B antibody [EPR16969] (AB203835), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ACTR1B antibody [EPR16969] (AB203835), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/10000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/250
Notes

-

Tested
Tested

Species
Human
Dilution info
1/300
Notes

-

Tested
Tested

Species
Human
Dilution info
1/300
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Component of a multi-subunit complex involved in microtubule based vesicle motility. It is associated with the centrosome.

Alternative names

CTRN2, ACTR1B, Beta-centractin, Actin-related protein 1B, ARP1B

Recommended products

Rabbit Recombinant Monoclonal ACTR1B antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR16969
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ACTR1B also known as Actin-Related Protein 1B is part of the ACTR family and plays a mechanical role in cellular processes. It has a molecular mass of approximately 45 kDa. ACTR1B expresses in various tissues including the brain and the testis. The protein is a component of the dynactin complex which associates with microtubules and is essential for dynein-mediated transport within cells.

Biological function summary

ACTR1B supports cellular activities such as intracellular transport and maintenance of cytoskeletal structure. It functions as a part of the dynactin complex a group of proteins that includes p150Glued and p50. This complex has a significant role in the function of cytoplasmic dynein a motor protein responsible for vesicle trafficking mitotic spindle organization and chromosome movement during cell division.

Pathways

ACTR1B contributes to intracellular transport and cytoskeletal regulation through its involvement in the dynactin complex. It directly participates in the dynein-dynactin pathway where it interacts with proteins like LIS1 and Ndel1 to facilitate microtubule-based movements. ACTR1B also plays a part in the endosome sorting pathway affecting proteins related to transport and sorting of intracellular cargo.

Associated diseases and disorders

Faulty ACTR1B function can contribute to neurodegenerative diseases and developmental disorders. For example its connection to dynein pathway disruptions links ACTR1B to defects in nervous system development potentially causing intellectual disabilities and lissencephaly. The protein's role in intracellular trafficking involves it with cellular stress responses linking it to some neurodegenerative diseases through proteins like tau and huntingtin.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-ACTR1B antibody [EPR16969] (ab203835), expandable thumbnail

    Western blot - Anti-ACTR1B antibody [EPR16969] (ab203835)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-ACTR1B antibody [EPR16969] (ab203835) at 1/10000 dilution

    Lane 1: MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate at 10 µg

    Lane 2: HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 10 µg

    Lane 3: 293 (Human epithelial cells from embryonic kidney) whole cell lysate at 10 µg

    Lane 4: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa

    Observed band size: 42 kDa

    Exposure time: 10s

  • Western blot - Anti-ACTR1B antibody [EPR16969] (ab203835), expandable thumbnail

    Western blot - Anti-ACTR1B antibody [EPR16969] (ab203835)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-ACTR1B antibody [EPR16969] (ab203835) at 1/10000 dilution

    Lane 1: Human fetal brain lysate at 10 µg

    Lane 2: Human fetal kidney lysate at 10 µg

    Lane 3: Human fetal spleen lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 42 kDa

    Observed band size: 42 kDa

    Exposure time: 15s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTR1B antibody [EPR16969] (ab203835), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTR1B antibody [EPR16969] (ab203835)

    Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling ACTR1B with ab203835 at 1/300 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasmic staining on Human cervix carcinoma tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-ACTR1B antibody [EPR16969] (ab203835), expandable thumbnail

    Western blot - Anti-ACTR1B antibody [EPR16969] (ab203835)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Lane 1: Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] ab203833) at 1/1000 dilution

    Lane 1: Western blot - Anti-ACTR1B antibody [EPR16969] (ab203835) at 1/1000 dilution

    All lanes: Full length Human ACTR1A recombination protein at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 42 kDa

    Observed band size: 42 kDa

    Exposure time: 3s

  • Immunocytochemistry/ Immunofluorescence - Anti-ACTR1B antibody [EPR16969] (ab203835), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ACTR1B antibody [EPR16969] (ab203835)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling ACTR1B with ab203835 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab203835 at 1/250 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.

    -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

  • Immunoprecipitation - Anti-ACTR1B antibody [EPR16969] (ab203835), expandable thumbnail

    Immunoprecipitation - Anti-ACTR1B antibody [EPR16969] (ab203835)

    ACTR1B was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab203835 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab203835 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1500 dilution.

    Lane 1: HeLa whole cell lysate 10ug (Input). Lane 2: ab203835 IP in HeLa whole cell lysate. Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab203835 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds.

    All lanes: Immunoprecipitation - Anti-ACTR1B antibody [EPR16969] (ab203835)

    Predicted band size: 42 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ACTR1B antibody [EPR16969] (ab203835), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ACTR1B antibody [EPR16969] (ab203835)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling ACTR1B with ab203835 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on MCF7 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab203835 at 1/250 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.

    -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-ACTR1B antibody [EPR16969] (ab203835), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ACTR1B antibody [EPR16969] (ab203835)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling ACTR1Bwith ab203835 at 1/300 dilution (red) compared with a rabbit monoclonal IgG isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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