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AB250628

Anti-ACTR3B antibody [EPR14091] - BSA and Azide free

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Rabbit Recombinant Monoclonal ACTR3B antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

View Alternative Names

ARP11, ARP4, ACTR3B, Actin-related protein 3B, ARP3-beta, Actin-like protein 3B, Actin-related protein ARP4

2 Images
Immunoprecipitation - Anti-ACTR3B antibody [EPR14091] - BSA and Azide free (AB250628)
  • IP

Supplier Data

Immunoprecipitation - Anti-ACTR3B antibody [EPR14091] - BSA and Azide free (AB250628)

This data was developed using ab182577, the same antibody clone in a different buffer formulation.

Immunoprecipitation analysis of Human fetal brain lysate labeling ACTR3B using ab182577 at 1/50 dilution. A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody.

All lanes:

Immunoprecipitation - Anti-ACTR3B antibody [EPR14091] (<a href='/en-us/products/primary-antibodies/actr3b-antibody-epr14091-ab182577'>ab182577</a>)

Predicted band size: 48 kDa

false

Western blot - Anti-ACTR3B antibody [EPR14091] - BSA and Azide free (AB250628)
  • WB

Supplier Data

Western blot - Anti-ACTR3B antibody [EPR14091] - BSA and Azide free (AB250628)

This data was developed using ab182577, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ACTR3B antibody [EPR14091] (<a href='/en-us/products/primary-antibodies/actr3b-antibody-epr14091-ab182577'>ab182577</a>) at 1/5000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

K562 cell lysate at 20 µg

Lane 3:

A431 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 48 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14091

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab250628 is the carrier-free version of ab182577.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ACTR3B also known as Actin-related protein 3B is a component of the ARP2/3 complex which contains approximately 47 kDa. Mechanically ACTR3B plays a role in the regulation of actin polymerization which is essential for the formation of branched actin networks. ACTR3B expression occurs in various tissues with prominent levels in cells requiring dynamic cytoskeletal changes. It interacts closely with other proteins of the ARP2/3 complex to drive actin network formation.
Biological function summary

ACTR3B influences cellular processes related to the maintenance of cell shape and motility by being part of the ARP2/3 complex. This complex assists in the nucleation of new actin filaments contributing to the dynamic remodeling of the actin cytoskeleton. The activity of ACTR3B impacts cell migration cell division and intracellular transport by reorganizing actin structures. ACTR3B's role within the ARP2/3 complex underlines its involvement in cellular projects demanding changes to cell morphology and interaction with the environment.

Pathways

The involvement of ACTR3B in the regulation of actin polymerization positions it within the WASP/WAVE signaling pathways. These pathways orchestrate the actin cytoskeletal dynamics that direct important cellular movements such as lamellipodia formation. ACTR3B works alongside proteins like WASP (Wiskott-Aldrich Syndrome Protein) in its pathway activities supporting coordinated cellular responses to external and internal signals through cytoskeletal rearrangement.

ACTR3B's association with actin polymerization links it to cancers and immune system diseases including Wiskott-Aldrich syndrome. These diseases often involve defects in cell movement and signaling due to impaired actin dynamics. In the context of cancer altered expression or mutation of ACTR3B may support metastatic processes through disrupted cell adhesion and migration. Additionally ACTR3B's connection with WASP highlights its potential role in disorders where actin cytoskeletal defects significantly affect immune responses.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in the organization of the actin cytoskeleton. May function as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. May decrease the metastatic potential of tumors.
See full target information ACTR3B
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Product promise

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