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Rabbit Polyclonal ACY-1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human Aminoacylase-1 aa 50-350.

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Images

Western blot - Anti-ACY-1 antibody (AB189399), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACY-1 antibody (AB189399), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ACY-1 antibody (AB189399), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.79% Tris glycine

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human Aminoacylase-1 aa 50-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q03154

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Tested
Tested

Species

Human

Dilution info

10 µg/mL

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/200.00000

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Catalyzes the hydrolysis of N-acetylated amino acids to acetate and free amino acids.

Alternative names

Recommended products

Rabbit Polyclonal ACY-1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human Aminoacylase-1 aa 50-350.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human Aminoacylase-1 aa 50-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q03154
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The protein ACY-1 also known as aminoacylase 1 or ACY plays a significant role in the deacetylation process of N-acyl amino acids. ACY-1 is a zinc-binding enzyme and has a molecular weight of approximately 45 kDa. This enzyme is expressed in various tissues including the liver and kidney. ACY-1 is essential for amino acid metabolism by catalyzing the hydrolysis of N-acyl-L-amino acids into L-amino acids and free fatty acids.

Biological function summary

The aminoacylase 1 enzyme functions in detoxification and metabolic regulation. It is not typically part of large protein complexes but acts independently to regulate amino acid and acetyl-CoA pathways. The activity facilitates the recycling of acyl groups and amino acids which is important for maintaining cellular homeostasis.

Pathways

Aminoacylase 1 is involved in amino acid catabolism and lipid metabolism pathways. In these processes ACY-1 interacts with proteins such as acetyl-CoA synthetase in the conversion of excess acyl-CoAs into free fatty acids and CoA preventing the build-up of toxic metabolites. It also has an indirect relationship with the urea cycle by ensuring the availability of free amino acids for further metabolism.

Associated diseases and disorders

Alterations in ACY-1 function relate to neurological disorders and metabolic syndromes. For instance defects in ACY-1 have been associated with Canavan disease a condition affecting the brain. The protein's activity imbalance might also link to metabolic disorders where its interaction with proteins like aspartoacylase comes into play further affecting the acetylation status and metabolic regulation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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3 product images

  • Western blot - Anti-ACY-1 antibody (ab189399), expandable thumbnail

    Western blot - Anti-ACY-1 antibody (ab189399)

    All lanes: Western blot - Anti-ACY-1 antibody (ab189399) at 1/1000 dilution

    All lanes: H1299 whole cell lysate at 30 µg

    Predicted band size: 46 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACY-1 antibody (ab189399), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACY-1 antibody (ab189399)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling ACY-1 using ab189399 at 10 μg/ml.

  • Immunocytochemistry/ Immunofluorescence - Anti-ACY-1 antibody (ab189399), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ACY-1 antibody (ab189399)

    Immunofluorescence analysis of paraformaldehyde-fixed A431 cells labeling ACY-1 using ab189399 at a 1/200 dilution (upper panel) and merged with DNA probe (lower panel)

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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